Striking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samples

Título traducido de la contribución: Divergencia sorprendente en las secuencias de nucleótidos de toxoplasma ROP16 de muestras humanas y de carne

Catalina Alvarez, Alejandra De-La-Torre, Mónica Vargas, Claudia Herrera, Lored Dayana Uribe-Huertas, Fabiana Lora, Jorge Enrique Gómez-Marín

Resultado de la investigación: Contribución a RevistaArtículo

13 Citas (Scopus)

Resumen


Antecedente. ROP16 es una proteína quinasa de Toxoplasma gondii identificada en el modelo de ratón como un marcador virulento, pero se desconoce si este hallazgo es relevante en la toxoplasmosis humana. Métodos. Obtuvimos la secuencia de ADN del locus Toxoplasma ROP16 en muestras de 12 pacientes con toxoplasmosis ocular, 1 muestra de un paciente con toxoplasmosis congénita, 22 muestras de soldados que operan en la selva, 2 muestras de soldados urbanos y 10 muestras de carne para consumo humano. Se realizó un ensayo inmunoabsorbente ligado a enzimas específico para anticuerpos contra el péptido ROP16 virulento de ratón en 46 muestras de suero de pacientes con toxoplasmosis ocular y en 28 muestras de suero de pacientes con infección crónica asintomática, de los cuales 19 tenían infección congénita y 11 tenían linfadenitis toxoplásmica. . Resultados. Encontramos una notable divergencia de las secuencias de nucleótidos ROP16. Diez de 12 secuencias (83,3%) de pacientes con toxoplasmosis ocular se agruparon con las de cepas virulentas de ratón, mientras que 7 de 7 secuencias ROP16 (100%) de la carne se agruparon con las de cepas avirulentas de ratón. Solo 11 de 104 muestras de suero (10.5%) tenían anticuerpos específicos contra el péptido virulento de ratón, y no hubo asociación entre las formas clínicas y los resultados positivos de los ensayos serológicos. Conclusiones La mayoría de las secuencias de nucleótidos ROP16 de pacientes colombianos con toxoplasmosis ocular pertenecían al grupo de cepas virulentas de ratón
Idioma originalEnglish (US)
Páginas (desde-hasta)2006-2013
Número de páginas8
PublicaciónJournal of Infectious Diseases
DOI
EstadoPublished - ene 1 2015

Huella dactilar

Toxoplasma
Ocular Toxoplasmosis
Meat
Military Personnel
Serum
Congenital Toxoplasmosis
Lymphadenitis
Peptides
Asymptomatic Infections
Antibodies
Toxoplasmosis
Protein Kinases
Enzyme-Linked Immunosorbent Assay
Infection

Citar esto

Alvarez, C., De-La-Torre, A., Vargas, M., Herrera, C., Uribe-Huertas, L. D., Lora, F., & Gómez-Marín, J. E. (2015). Striking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samples. Journal of Infectious Diseases, 2006-2013. https://doi.org/10.1093/infdis/jiu833
Alvarez, Catalina ; De-La-Torre, Alejandra ; Vargas, Mónica ; Herrera, Claudia ; Uribe-Huertas, Lored Dayana ; Lora, Fabiana ; Gómez-Marín, Jorge Enrique. / Striking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samples. En: Journal of Infectious Diseases. 2015 ; pp. 2006-2013.
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title = "Striking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samples",
abstract = "Background. ROP16 is a protein kinase of Toxoplasma gondii identified in the mouse model as a virulent marker, but it is unknown whether this finding is relevant in human toxoplasmosis. Methods. We obtained the Toxoplasma ROP16 locus DNA sequence in samples from 12 patients with ocular toxoplasmosis, 1 sample from a patient with congenital toxoplasmosis, 22 samples from soldiers operating in the jungle, 2 samples from urban soldiers, and 10 samples from meat for human consumption. An enzyme-linked immunosorbent assay specific for antibodies against the ROP16 mouse-virulent peptide was performed in 46 serum specimens from patients with ocular toxoplasmosis and in 28 serum specimens from patients with chronic asymptomatic infection, of whom 19 had congenital infection and 11 had toxoplasmic lymphadenitis. Results. We found a striking divergence of the ROP16 nucleotide sequences. Ten of 12 sequences (83.3{\%}) from patients with ocular toxoplasmosis clustered with those of mouse-virulent strains, whereas 7 of 7 ROP16 sequences (100{\%}) from meat were clustered with those of mouse-avirulent strains. Only 11 of 104 serum specimens (10.5{\%}) had specific antibodies against the mouse-virulent peptide, and there was no association between clinical forms and positive results of serological assays. Conclusions. The majority of ROP16 nucleotide sequences from Colombian patients with ocular toxoplasmosis belonged to the group of mouse-virulent strains.",
author = "Catalina Alvarez and Alejandra De-La-Torre and M{\'o}nica Vargas and Claudia Herrera and Uribe-Huertas, {Lored Dayana} and Fabiana Lora and G{\'o}mez-Mar{\'i}n, {Jorge Enrique}",
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Striking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samples. / Alvarez, Catalina; De-La-Torre, Alejandra; Vargas, Mónica; Herrera, Claudia; Uribe-Huertas, Lored Dayana; Lora, Fabiana; Gómez-Marín, Jorge Enrique.

En: Journal of Infectious Diseases, 01.01.2015, p. 2006-2013.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Striking divergence in toxoplasma ROP16 nucleotide sequences from human and meat samples

AU - Alvarez, Catalina

AU - De-La-Torre, Alejandra

AU - Vargas, Mónica

AU - Herrera, Claudia

AU - Uribe-Huertas, Lored Dayana

AU - Lora, Fabiana

AU - Gómez-Marín, Jorge Enrique

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Background. ROP16 is a protein kinase of Toxoplasma gondii identified in the mouse model as a virulent marker, but it is unknown whether this finding is relevant in human toxoplasmosis. Methods. We obtained the Toxoplasma ROP16 locus DNA sequence in samples from 12 patients with ocular toxoplasmosis, 1 sample from a patient with congenital toxoplasmosis, 22 samples from soldiers operating in the jungle, 2 samples from urban soldiers, and 10 samples from meat for human consumption. An enzyme-linked immunosorbent assay specific for antibodies against the ROP16 mouse-virulent peptide was performed in 46 serum specimens from patients with ocular toxoplasmosis and in 28 serum specimens from patients with chronic asymptomatic infection, of whom 19 had congenital infection and 11 had toxoplasmic lymphadenitis. Results. We found a striking divergence of the ROP16 nucleotide sequences. Ten of 12 sequences (83.3%) from patients with ocular toxoplasmosis clustered with those of mouse-virulent strains, whereas 7 of 7 ROP16 sequences (100%) from meat were clustered with those of mouse-avirulent strains. Only 11 of 104 serum specimens (10.5%) had specific antibodies against the mouse-virulent peptide, and there was no association between clinical forms and positive results of serological assays. Conclusions. The majority of ROP16 nucleotide sequences from Colombian patients with ocular toxoplasmosis belonged to the group of mouse-virulent strains.

AB - Background. ROP16 is a protein kinase of Toxoplasma gondii identified in the mouse model as a virulent marker, but it is unknown whether this finding is relevant in human toxoplasmosis. Methods. We obtained the Toxoplasma ROP16 locus DNA sequence in samples from 12 patients with ocular toxoplasmosis, 1 sample from a patient with congenital toxoplasmosis, 22 samples from soldiers operating in the jungle, 2 samples from urban soldiers, and 10 samples from meat for human consumption. An enzyme-linked immunosorbent assay specific for antibodies against the ROP16 mouse-virulent peptide was performed in 46 serum specimens from patients with ocular toxoplasmosis and in 28 serum specimens from patients with chronic asymptomatic infection, of whom 19 had congenital infection and 11 had toxoplasmic lymphadenitis. Results. We found a striking divergence of the ROP16 nucleotide sequences. Ten of 12 sequences (83.3%) from patients with ocular toxoplasmosis clustered with those of mouse-virulent strains, whereas 7 of 7 ROP16 sequences (100%) from meat were clustered with those of mouse-avirulent strains. Only 11 of 104 serum specimens (10.5%) had specific antibodies against the mouse-virulent peptide, and there was no association between clinical forms and positive results of serological assays. Conclusions. The majority of ROP16 nucleotide sequences from Colombian patients with ocular toxoplasmosis belonged to the group of mouse-virulent strains.

U2 - 10.1093/infdis/jiu833

DO - 10.1093/infdis/jiu833

M3 - Article

SP - 2006

EP - 2013

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

ER -