TY - JOUR
T1 - Dirofilaria immitis in pet dogs from the metropolitan area of the Colombian Caribbean
AU - Badillo-Viloria, Maria A.
AU - García-Bocanegra, Ignacio
AU - de Lavalle-Galvis, Rodrigo J.
AU - Martínez, Remigio
AU - de la Rosa-Jaramillo, Steffania
AU - Castillo-Castañeda, Adriana
AU - Ramírez, Juan D.
AU - Cano-Terriza, David
N1 - Funding Information:
The authors thank the veterinarians and the personal of the different veterinary clinics that participated in the collection of samples. We thank Virbac Colombia Ltda., for kindly providing the Speed Diro™ rapid assays used in the study and the Direccion de Investigación e Innovacion from Universidad del Rosario for funding the sequencing service. RMP was supported by a postdoctoral contract Margarita Salas Reference MS-23 ( University of Extremadura ) from the Program of Requalification of the Spanish University System (Spanish Ministry of Universities) financed by the European Union - NextGenerationEU. This work has been partially funded by CIBER -Consorcio Centro de Investigación Biomédica en Red- (CB 2021), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea – NextGenerationEU .
Funding Information:
The authors thank the veterinarians and the personal of the different veterinary clinics that participated in the collection of samples. We thank Virbac Colombia Ltda. for kindly providing the Speed Diro™ rapid assays used in the study and the Direccion de Investigación e Innovacion from Universidad del Rosario for funding the sequencing service. RMP was supported by a postdoctoral contract Margarita Salas Reference MS-23 (University of Extremadura) from the Program of Requalification of the Spanish University System (Spanish Ministry of Universities) financed by the European Union - NextGenerationEU. This work has been partially funded by CIBER -Consorcio Centro de Investigación Biomédica en Red- (CB 2021), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea – NextGenerationEU.
Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2023/11
Y1 - 2023/11
N2 - A cross-sectional study was carried out to determine the frequency and factors associated with Dirofilaria immitis infection in pet dogs in the metropolitan area of the Colombian Caribbean (northern Colombia). A total of 173 dogs were analyzed by a commercial rapid immunochromatographic test (RIT) and a nested PCR of the cytochrome oxidase subunit I gene, in parallel. Ninety-two (53.2%) of the dogs showed positive results to the RIT, while 59 (34.1%) animals had D. immitis DNA by PCR. Positivity to one or both diagnostic techniques was detected in 104 (60.1%; CI95%: 53.8–67.4) of the sampled dogs. In PCR-positive dogs, phylogenetic analyses evidenced high nucleotide identity (100%) with sequences previously obtained from mosquitoes, dogs and other mammals in different countries. Exercise intolerance (p = 0.002; OR: 2.33; CI95%: 1.37–3.96) and thrombocytopenia (p = < 0.001; OR: 1.95; CI95%: 1.11–3.43) were the main factors associated with D. immitis infection in dogs. The high frequency of D. immitis in dogs indicates a wide distribution of this parasite in the metropolitan area of the Colombian Caribbean, which can be of animal and public health concern. Our results highlight the need to combine different methods to increase the diagnostic accuracy of D. immitis.
AB - A cross-sectional study was carried out to determine the frequency and factors associated with Dirofilaria immitis infection in pet dogs in the metropolitan area of the Colombian Caribbean (northern Colombia). A total of 173 dogs were analyzed by a commercial rapid immunochromatographic test (RIT) and a nested PCR of the cytochrome oxidase subunit I gene, in parallel. Ninety-two (53.2%) of the dogs showed positive results to the RIT, while 59 (34.1%) animals had D. immitis DNA by PCR. Positivity to one or both diagnostic techniques was detected in 104 (60.1%; CI95%: 53.8–67.4) of the sampled dogs. In PCR-positive dogs, phylogenetic analyses evidenced high nucleotide identity (100%) with sequences previously obtained from mosquitoes, dogs and other mammals in different countries. Exercise intolerance (p = 0.002; OR: 2.33; CI95%: 1.37–3.96) and thrombocytopenia (p = < 0.001; OR: 1.95; CI95%: 1.11–3.43) were the main factors associated with D. immitis infection in dogs. The high frequency of D. immitis in dogs indicates a wide distribution of this parasite in the metropolitan area of the Colombian Caribbean, which can be of animal and public health concern. Our results highlight the need to combine different methods to increase the diagnostic accuracy of D. immitis.
UR - https://www.scopus.com/pages/publications/85171996666
UR - https://www.scopus.com/pages/publications/85171996666#tab=citedBy
U2 - 10.1016/j.cimid.2023.102064
DO - 10.1016/j.cimid.2023.102064
M3 - Research Article
C2 - 37741216
AN - SCOPUS:85171996666
SN - 0147-9571
VL - 102
JO - Comparative Immunology, Microbiology and Infectious Diseases
JF - Comparative Immunology, Microbiology and Infectious Diseases
M1 - 102064
ER -
ODS 3: Salud y bienestar