Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients

Juan David Ramírez; Giovanny Herrera; Carlos Muskus; Claudia Mendez; María Clara Duque; Robert Butcher

doi:10.1016/j.ijid.2018.10.029

Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients

Juan David Ramírez, Giovanny Herrera, Carlos Muskus, Claudia Mendez, María Clara Duque, Robert Butcher

Universidad del Rosario

Producción científica: Contribución a una revista › Artículo de Investigación › revisión exhaustiva

10 Citas (Scopus)

Resumen

Aim: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. Methods: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to determine the analytical and diagnostic performance. Results: The limit of detection of the assay on the ddPCR platform was much higher than the published limit of detection of the same assay on the qPCR platform (100 vs 1 parasites/mL, respectively). Conclusion: While the performance of this assay in ddPCR format was acceptable for research purposes, it is not sufficient for clinical diagnostic purposes. The assay is more suited to the qPCR platform.

Idioma original	Inglés estadounidense
Páginas (desde-hasta)	1-3
Número de páginas	3
Publicación	International Journal of Infectious Diseases
Volumen	79
DOI	https://doi.org/10.1016/j.ijid.2018.10.029
Estado	Publicada - feb. 1 2019

Áreas temáticas de ASJC Scopus

Microbiología (médica)
Enfermedades infecciosas

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

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10.1016/j.ijid.2018.10.029

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title = "Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients",

abstract = "Aim: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. Methods: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to determine the analytical and diagnostic performance. Results: The limit of detection of the assay on the ddPCR platform was much higher than the published limit of detection of the same assay on the qPCR platform (100 vs 1 parasites/mL, respectively). Conclusion: While the performance of this assay in ddPCR format was acceptable for research purposes, it is not sufficient for clinical diagnostic purposes. The assay is more suited to the qPCR platform.",

author = "Ram{\'i}rez, {Juan David} and Giovanny Herrera and Carlos Muskus and Claudia Mendez and Duque, {Mar{\'i}a Clara} and Robert Butcher",

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doi = "10.1016/j.ijid.2018.10.029",

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Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients. / Ramírez, Juan David; Herrera, Giovanny; Muskus, Carlos et al.
En: International Journal of Infectious Diseases, Vol. 79, 01.02.2019, p. 1-3.

Producción científica: Contribución a una revista › Artículo de Investigación › revisión exhaustiva

TY - JOUR

T1 - Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients

AU - Ramírez, Juan David

AU - Herrera, Giovanny

AU - Muskus, Carlos

AU - Mendez, Claudia

AU - Duque, María Clara

AU - Butcher, Robert

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Aim: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. Methods: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to determine the analytical and diagnostic performance. Results: The limit of detection of the assay on the ddPCR platform was much higher than the published limit of detection of the same assay on the qPCR platform (100 vs 1 parasites/mL, respectively). Conclusion: While the performance of this assay in ddPCR format was acceptable for research purposes, it is not sufficient for clinical diagnostic purposes. The assay is more suited to the qPCR platform.

AB - Aim: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. Methods: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to determine the analytical and diagnostic performance. Results: The limit of detection of the assay on the ddPCR platform was much higher than the published limit of detection of the same assay on the qPCR platform (100 vs 1 parasites/mL, respectively). Conclusion: While the performance of this assay in ddPCR format was acceptable for research purposes, it is not sufficient for clinical diagnostic purposes. The assay is more suited to the qPCR platform.

UR - http://www.scopus.com/inward/record.url?scp=85057876716&partnerID=8YFLogxK

U2 - 10.1016/j.ijid.2018.10.029

DO - 10.1016/j.ijid.2018.10.029

M3 - Research Article

C2 - 30399446

AN - SCOPUS:85057876716

SN - 1201-9712

VL - 79

SP - 1

EP - 3

JO - International Journal of Infectious Diseases

JF - International Journal of Infectious Diseases

ER -

Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients

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Áreas temáticas de ASJC Scopus

ODS de las Naciones Unidas

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