Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients

Juan David Ramírez, Giovanny Herrera, Carlos Muskus, Claudia Mendez, María Clara Duque, Robert Butcher

Resultado de la investigación: Contribución a RevistaArtículo

Resumen

Aim: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. Methods: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to determine the analytical and diagnostic performance. Results: The limit of detection of the assay on the ddPCR platform was much higher than the published limit of detection of the same assay on the qPCR platform (100 vs 1 parasites/mL, respectively). Conclusion: While the performance of this assay in ddPCR format was acceptable for research purposes, it is not sufficient for clinical diagnostic purposes. The assay is more suited to the qPCR platform.

Idioma originalEnglish (US)
Páginas (desde-hasta)1-3
Número de páginas3
PublicaciónInternational Journal of Infectious Diseases
Volumen79
DOI
EstadoPublished - feb 1 2019

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Citar esto

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abstract = "Aim: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. Methods: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to determine the analytical and diagnostic performance. Results: The limit of detection of the assay on the ddPCR platform was much higher than the published limit of detection of the same assay on the qPCR platform (100 vs 1 parasites/mL, respectively). Conclusion: While the performance of this assay in ddPCR format was acceptable for research purposes, it is not sufficient for clinical diagnostic purposes. The assay is more suited to the qPCR platform.",
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Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients. / Ramírez, Juan David; Herrera, Giovanny; Muskus, Carlos; Mendez, Claudia; Duque, María Clara; Butcher, Robert.

En: International Journal of Infectious Diseases, Vol. 79, 01.02.2019, p. 1-3.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients

AU - Ramírez, Juan David

AU - Herrera, Giovanny

AU - Muskus, Carlos

AU - Mendez, Claudia

AU - Duque, María Clara

AU - Butcher, Robert

PY - 2019/2/1

Y1 - 2019/2/1

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AB - Aim: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. Methods: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to determine the analytical and diagnostic performance. Results: The limit of detection of the assay on the ddPCR platform was much higher than the published limit of detection of the same assay on the qPCR platform (100 vs 1 parasites/mL, respectively). Conclusion: While the performance of this assay in ddPCR format was acceptable for research purposes, it is not sufficient for clinical diagnostic purposes. The assay is more suited to the qPCR platform.

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