Detection by PCR of human papillomavirus in Colombia: Comparison of GP5+/6+ and MY09/11 primer sets

Milena Camargo, Sara Soto-De Leon, Ricardo Sanchez, Marina Munoz, Erika Vega, Magda Beltran, Antonio Perez-Prados, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo

    Resultado de la investigación: Contribución a RevistaArtículo

    22 Citas (Scopus)

    Resumen

    The aims of this study were to determine the prevalence of HPV infection and evaluate the concordance and performance of two primer sets for detecting single and multiple viral infections. A total of 1810 Colombian women were enrolled in the study, and molecular, cytological and epidemiological analyses were performed. Both concordance and performance of two different PCR amplification primer sets (GP5+/6+ and MY09/11) were assessed. The results showed that 60.2% of females with positive HPV DNA were infected by more than one viral type. The OR for multiple infections was 18.2 when using the MY09/11 primer set and 6.52 with the GP5+/6+ primer set. The results also showed an association between GP5+/6+ positivity and the severity of the disease regarding the cytological findings. It was also found that using a single primer set led to underestimating the prevalence for HPV infection. The simultaneous use of these primer sets is an important tool for the detection of HPV DNA, being equally relevant for identifying multiple infections and low viral DNA copies. This study highlights the importance of suitable assessment of HPV epidemiological profiles; screening programs must also be strengthened to broaden the coverage of the most vulnerable populations. ?? 2011 Elsevier B.V.
    Idioma originalEnglish (US)
    Páginas (desde-hasta)68-74
    Número de páginas7
    PublicaciónJournal of Virological Methods
    Volumen178
    N.º1-2
    DOI
    EstadoPublished - dic 2011

    Huella dactilar

    Colombia
    Polymerase Chain Reaction
    Infection
    DNA
    Viral DNA
    Vulnerable Populations
    Virus Diseases

    Citar esto

    Camargo, Milena ; Soto-De Leon, Sara ; Sanchez, Ricardo ; Munoz, Marina ; Vega, Erika ; Beltran, Magda ; Perez-Prados, Antonio ; Patarroyo, Manuel Elkin ; Patarroyo, Manuel Alfonso. / Detection by PCR of human papillomavirus in Colombia: Comparison of GP5+/6+ and MY09/11 primer sets. En: Journal of Virological Methods. 2011 ; Vol. 178, N.º 1-2. pp. 68-74.
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    title = "Detection by PCR of human papillomavirus in Colombia: Comparison of GP5+/6+ and MY09/11 primer sets",
    abstract = "The aims of this study were to determine the prevalence of HPV infection and evaluate the concordance and performance of two primer sets for detecting single and multiple viral infections. A total of 1810 Colombian women were enrolled in the study, and molecular, cytological and epidemiological analyses were performed. Both concordance and performance of two different PCR amplification primer sets (GP5+/6+ and MY09/11) were assessed. The results showed that 60.2{\%} of females with positive HPV DNA were infected by more than one viral type. The OR for multiple infections was 18.2 when using the MY09/11 primer set and 6.52 with the GP5+/6+ primer set. The results also showed an association between GP5+/6+ positivity and the severity of the disease regarding the cytological findings. It was also found that using a single primer set led to underestimating the prevalence for HPV infection. The simultaneous use of these primer sets is an important tool for the detection of HPV DNA, being equally relevant for identifying multiple infections and low viral DNA copies. This study highlights the importance of suitable assessment of HPV epidemiological profiles; screening programs must also be strengthened to broaden the coverage of the most vulnerable populations. ?? 2011 Elsevier B.V.",
    author = "Milena Camargo and {Soto-De Leon}, Sara and Ricardo Sanchez and Marina Munoz and Erika Vega and Magda Beltran and Antonio Perez-Prados and Patarroyo, {Manuel Elkin} and Patarroyo, {Manuel Alfonso}",
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    Camargo, M, Soto-De Leon, S, Sanchez, R, Munoz, M, Vega, E, Beltran, M, Perez-Prados, A, Patarroyo, ME & Patarroyo, MA 2011, 'Detection by PCR of human papillomavirus in Colombia: Comparison of GP5+/6+ and MY09/11 primer sets', Journal of Virological Methods, vol. 178, n.º 1-2, pp. 68-74. https://doi.org/10.1016/j.jviromet.2011.08.014

    Detection by PCR of human papillomavirus in Colombia: Comparison of GP5+/6+ and MY09/11 primer sets. / Camargo, Milena; Soto-De Leon, Sara; Sanchez, Ricardo; Munoz, Marina; Vega, Erika; Beltran, Magda; Perez-Prados, Antonio; Patarroyo, Manuel Elkin; Patarroyo, Manuel Alfonso.

    En: Journal of Virological Methods, Vol. 178, N.º 1-2, 12.2011, p. 68-74.

    Resultado de la investigación: Contribución a RevistaArtículo

    TY - JOUR

    T1 - Detection by PCR of human papillomavirus in Colombia: Comparison of GP5+/6+ and MY09/11 primer sets

    AU - Camargo, Milena

    AU - Soto-De Leon, Sara

    AU - Sanchez, Ricardo

    AU - Munoz, Marina

    AU - Vega, Erika

    AU - Beltran, Magda

    AU - Perez-Prados, Antonio

    AU - Patarroyo, Manuel Elkin

    AU - Patarroyo, Manuel Alfonso

    PY - 2011/12

    Y1 - 2011/12

    N2 - The aims of this study were to determine the prevalence of HPV infection and evaluate the concordance and performance of two primer sets for detecting single and multiple viral infections. A total of 1810 Colombian women were enrolled in the study, and molecular, cytological and epidemiological analyses were performed. Both concordance and performance of two different PCR amplification primer sets (GP5+/6+ and MY09/11) were assessed. The results showed that 60.2% of females with positive HPV DNA were infected by more than one viral type. The OR for multiple infections was 18.2 when using the MY09/11 primer set and 6.52 with the GP5+/6+ primer set. The results also showed an association between GP5+/6+ positivity and the severity of the disease regarding the cytological findings. It was also found that using a single primer set led to underestimating the prevalence for HPV infection. The simultaneous use of these primer sets is an important tool for the detection of HPV DNA, being equally relevant for identifying multiple infections and low viral DNA copies. This study highlights the importance of suitable assessment of HPV epidemiological profiles; screening programs must also be strengthened to broaden the coverage of the most vulnerable populations. ?? 2011 Elsevier B.V.

    AB - The aims of this study were to determine the prevalence of HPV infection and evaluate the concordance and performance of two primer sets for detecting single and multiple viral infections. A total of 1810 Colombian women were enrolled in the study, and molecular, cytological and epidemiological analyses were performed. Both concordance and performance of two different PCR amplification primer sets (GP5+/6+ and MY09/11) were assessed. The results showed that 60.2% of females with positive HPV DNA were infected by more than one viral type. The OR for multiple infections was 18.2 when using the MY09/11 primer set and 6.52 with the GP5+/6+ primer set. The results also showed an association between GP5+/6+ positivity and the severity of the disease regarding the cytological findings. It was also found that using a single primer set led to underestimating the prevalence for HPV infection. The simultaneous use of these primer sets is an important tool for the detection of HPV DNA, being equally relevant for identifying multiple infections and low viral DNA copies. This study highlights the importance of suitable assessment of HPV epidemiological profiles; screening programs must also be strengthened to broaden the coverage of the most vulnerable populations. ?? 2011 Elsevier B.V.

    U2 - 10.1016/j.jviromet.2011.08.014

    DO - 10.1016/j.jviromet.2011.08.014

    M3 - Article

    VL - 178

    SP - 68

    EP - 74

    JO - Journal of Virological Methods

    JF - Journal of Virological Methods

    SN - 0166-0934

    IS - 1-2

    ER -