Simultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region

Título traducido de la contribución: Detección simultánea de mutaciones asociadas a resistencia de Plasmodium vivax dhfr, dhps, mdr1 y crt-o en la región amazónica colombiana.

Juan Ricardo Cubides, Paola Andrea Camargo-Ayala, Carlos Hernando Niño, Diego Garzón-Ospina, Anggie Ortega-Ortegón, Estefany Ospina-Cantillo, María Fernanda Orduz-Durán, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

1 Cita (Scopus)

Resumen

ANTECEDENTES:
La malaria sigue siendo un problema de salud pública en todo el mundo. Plasmodium vivax es la especie que causa el mayor número de casos de malaria en Asia y América del Sur. Debido a la falta de una vacuna antipalúdica completamente eficaz, el control de esta enfermedad se ha basado en el manejo de los vectores de transmisión, el diagnóstico rápido y el tratamiento adecuado. Sin embargo, la resistencia de los parásitos a los medicamentos antipalúdicos se ha convertido en un reto importante que aún no se ha superado. El objetivo de este estudio fue determinar las mutaciones y haplotipos de los genes pvmdr1, pvdhfr, pvdhps y pvcrt-o a partir de muestras de campo obtenidas de un área endémica de la región amazónica colombiana.

MÉTODOS:
Cincuenta muestras de ADN de parásitos infectados por una sola cepa de P. vivax de pacientes sintomáticos del departamento de Amazonas en Colombia fueron analizadas por PCR y los genes pvdhfr, pvdhps, pvmdr1 y pvcrt-o fueron secuenciados. Se calcularon estimadores de diversidad a partir de las secuencias y se obtuvieron los haplotipos que circulan en la región amazónica colombiana.

CONCLUSIÓN:
Los genes pvdhfr, pvdhps, pvmdr1 y pvcrt-o en la región amazónica colombiana se caracterizan por su baja diversidad genética. Se encontraron algunas mutaciones asociadas a la resistencia circulando en esta población. También se están notificando nuevas variantes. Se localizó una señal de barrido selectivo en los genes pvdhfr y pvmdr1, lo que sugiere que estas mutaciones (o algunas de ellas) podrían estar proporcionando una ventaja adaptativa.
Idioma originalEnglish (US)
Páginas (desde-hasta)130
Número de páginas9
PublicaciónMalaria Journal
Volumen17
N.º1
DOI
EstadoPublished - mar 27 2018
Publicado de forma externa

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Cubides, Juan Ricardo ; Camargo-Ayala, Paola Andrea ; Niño, Carlos Hernando ; Garzón-Ospina, Diego ; Ortega-Ortegón, Anggie ; Ospina-Cantillo, Estefany ; Orduz-Durán, María Fernanda ; Patarroyo, Manuel Elkin ; Patarroyo, Manuel Alfonso. / Simultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region. En: Malaria Journal. 2018 ; Vol. 17, N.º 1. pp. 130.
@article{934ed5e8ed8c44a19416ebec020bbcee,
title = "Simultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region",
abstract = "BACKGROUND: Malaria continues being a public health problem worldwide. Plasmodium vivax is the species causing the largest number of cases of malaria in Asia and South America. Due to the lack of a completely effective anti-malarial vaccine, controlling this disease has been based on transmission vector management, rapid diagnosis and suitable treatment. However, parasite resistance to anti-malarial drugs has become a major yet-to-be-overcome challenge. This study was thus aimed at determining pvmdr1, pvdhfr, pvdhps and pvcrt-o gene mutations and haplotypes from field samples obtained from an endemic area in the Colombian Amazonian region.METHODS: Fifty samples of parasite DNA infected by a single P. vivax strain from symptomatic patients from the Amazonas department in Colombia were analysed by PCR and the pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes were sequenced. Diversity estimators were calculated from the sequences and the haplotypes circulating in the Colombian Amazonian region were obtained.CONCLUSION: pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes in the Colombian Amazonian region are characterized by low genetic diversity. Some resistance-associated mutations were found circulating in this population. New variants are also being reported. A selective sweep signal was located in pvdhfr and pvmdr1 genes, suggesting that these mutations (or some of them) could be providing an adaptive advantage.",
author = "Cubides, {Juan Ricardo} and Camargo-Ayala, {Paola Andrea} and Ni{\~n}o, {Carlos Hernando} and Diego Garz{\'o}n-Ospina and Anggie Ortega-Orteg{\'o}n and Estefany Ospina-Cantillo and Orduz-Dur{\'a}n, {Mar{\'i}a Fernanda} and Patarroyo, {Manuel Elkin} and Patarroyo, {Manuel Alfonso}",
year = "2018",
month = "3",
day = "27",
doi = "10.1186/s12936-018-2286-5",
language = "English (US)",
volume = "17",
pages = "130",
journal = "Malaria Journal",
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Cubides, JR, Camargo-Ayala, PA, Niño, CH, Garzón-Ospina, D, Ortega-Ortegón, A, Ospina-Cantillo, E, Orduz-Durán, MF, Patarroyo, ME & Patarroyo, MA 2018, 'Simultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region', Malaria Journal, vol. 17, n.º 1, pp. 130. https://doi.org/10.1186/s12936-018-2286-5

Simultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region. / Cubides, Juan Ricardo; Camargo-Ayala, Paola Andrea; Niño, Carlos Hernando; Garzón-Ospina, Diego; Ortega-Ortegón, Anggie; Ospina-Cantillo, Estefany; Orduz-Durán, María Fernanda; Patarroyo, Manuel Elkin; Patarroyo, Manuel Alfonso.

En: Malaria Journal, Vol. 17, N.º 1, 27.03.2018, p. 130.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Simultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region

AU - Cubides, Juan Ricardo

AU - Camargo-Ayala, Paola Andrea

AU - Niño, Carlos Hernando

AU - Garzón-Ospina, Diego

AU - Ortega-Ortegón, Anggie

AU - Ospina-Cantillo, Estefany

AU - Orduz-Durán, María Fernanda

AU - Patarroyo, Manuel Elkin

AU - Patarroyo, Manuel Alfonso

PY - 2018/3/27

Y1 - 2018/3/27

N2 - BACKGROUND: Malaria continues being a public health problem worldwide. Plasmodium vivax is the species causing the largest number of cases of malaria in Asia and South America. Due to the lack of a completely effective anti-malarial vaccine, controlling this disease has been based on transmission vector management, rapid diagnosis and suitable treatment. However, parasite resistance to anti-malarial drugs has become a major yet-to-be-overcome challenge. This study was thus aimed at determining pvmdr1, pvdhfr, pvdhps and pvcrt-o gene mutations and haplotypes from field samples obtained from an endemic area in the Colombian Amazonian region.METHODS: Fifty samples of parasite DNA infected by a single P. vivax strain from symptomatic patients from the Amazonas department in Colombia were analysed by PCR and the pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes were sequenced. Diversity estimators were calculated from the sequences and the haplotypes circulating in the Colombian Amazonian region were obtained.CONCLUSION: pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes in the Colombian Amazonian region are characterized by low genetic diversity. Some resistance-associated mutations were found circulating in this population. New variants are also being reported. A selective sweep signal was located in pvdhfr and pvmdr1 genes, suggesting that these mutations (or some of them) could be providing an adaptive advantage.

AB - BACKGROUND: Malaria continues being a public health problem worldwide. Plasmodium vivax is the species causing the largest number of cases of malaria in Asia and South America. Due to the lack of a completely effective anti-malarial vaccine, controlling this disease has been based on transmission vector management, rapid diagnosis and suitable treatment. However, parasite resistance to anti-malarial drugs has become a major yet-to-be-overcome challenge. This study was thus aimed at determining pvmdr1, pvdhfr, pvdhps and pvcrt-o gene mutations and haplotypes from field samples obtained from an endemic area in the Colombian Amazonian region.METHODS: Fifty samples of parasite DNA infected by a single P. vivax strain from symptomatic patients from the Amazonas department in Colombia were analysed by PCR and the pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes were sequenced. Diversity estimators were calculated from the sequences and the haplotypes circulating in the Colombian Amazonian region were obtained.CONCLUSION: pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes in the Colombian Amazonian region are characterized by low genetic diversity. Some resistance-associated mutations were found circulating in this population. New variants are also being reported. A selective sweep signal was located in pvdhfr and pvmdr1 genes, suggesting that these mutations (or some of them) could be providing an adaptive advantage.

U2 - 10.1186/s12936-018-2286-5

DO - 10.1186/s12936-018-2286-5

M3 - Article

VL - 17

SP - 130

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

IS - 1

ER -

Cubides JR, Camargo-Ayala PA, Niño CH, Garzón-Ospina D, Ortega-Ortegón A, Ospina-Cantillo E y otros. Simultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region. Malaria Journal. 2018 mar 27;17(1):130. https://doi.org/10.1186/s12936-018-2286-5