Description of Leishmania species among dogs and humans in Colombian Visceral Leishmaniasis outbreaks

Giovanny Herrera, Adriana Higuera, Luz Helena Patiño, Martha S. Ayala, Juan David Ramírez

Resultado de la investigación: Contribución a RevistaArtículo

2 Citas (Scopus)

Resumen

We report the species detected in dogs and humans from outbreaks of visceral leishmaniasis in Colombia. In this study, 91 sera from patients (n = 38) and dogs (n = 53) diagnosed with visceral leishmaniasis using IFAT were analyzed to determine the causative species. DNA extraction, PCR amplification, DNA sequencing and species identification was performed. Results were obtained with 13 of the sera. A phylogenetic tree and a network of haplotypes were constructed. Leishmania infantum chagasi (11/13), Leishmania braziliensis (1/13) and Leishmania amazonensis (1/13) were identified as the circulating species and genetic variability in one of the L. infantum chagasi strains was demonstrated. This is the first study describing Leishmania species in outbreaks of visceral leishmaniasis in Colombia.

Idioma originalEnglish (US)
Páginas (desde-hasta)135-138
Número de páginas4
PublicaciónInfection, Genetics and Evolution
Volumen64
DOI
EstadoPublished - oct 1 2018

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Citar esto

Herrera, Giovanny ; Higuera, Adriana ; Patiño, Luz Helena ; Ayala, Martha S. ; Ramírez, Juan David. / Description of Leishmania species among dogs and humans in Colombian Visceral Leishmaniasis outbreaks. En: Infection, Genetics and Evolution. 2018 ; Vol. 64. pp. 135-138.
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Description of Leishmania species among dogs and humans in Colombian Visceral Leishmaniasis outbreaks. / Herrera, Giovanny; Higuera, Adriana; Patiño, Luz Helena; Ayala, Martha S.; Ramírez, Juan David.

En: Infection, Genetics and Evolution, Vol. 64, 01.10.2018, p. 135-138.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Description of Leishmania species among dogs and humans in Colombian Visceral Leishmaniasis outbreaks

AU - Herrera, Giovanny

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AU - Patiño, Luz Helena

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AU - Ramírez, Juan David

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N2 - We report the species detected in dogs and humans from outbreaks of visceral leishmaniasis in Colombia. In this study, 91 sera from patients (n = 38) and dogs (n = 53) diagnosed with visceral leishmaniasis using IFAT were analyzed to determine the causative species. DNA extraction, PCR amplification, DNA sequencing and species identification was performed. Results were obtained with 13 of the sera. A phylogenetic tree and a network of haplotypes were constructed. Leishmania infantum chagasi (11/13), Leishmania braziliensis (1/13) and Leishmania amazonensis (1/13) were identified as the circulating species and genetic variability in one of the L. infantum chagasi strains was demonstrated. This is the first study describing Leishmania species in outbreaks of visceral leishmaniasis in Colombia.

AB - We report the species detected in dogs and humans from outbreaks of visceral leishmaniasis in Colombia. In this study, 91 sera from patients (n = 38) and dogs (n = 53) diagnosed with visceral leishmaniasis using IFAT were analyzed to determine the causative species. DNA extraction, PCR amplification, DNA sequencing and species identification was performed. Results were obtained with 13 of the sera. A phylogenetic tree and a network of haplotypes were constructed. Leishmania infantum chagasi (11/13), Leishmania braziliensis (1/13) and Leishmania amazonensis (1/13) were identified as the circulating species and genetic variability in one of the L. infantum chagasi strains was demonstrated. This is the first study describing Leishmania species in outbreaks of visceral leishmaniasis in Colombia.

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