TY - JOUR
T1 - Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability
AU - Avendaño, Catalina
AU - Jenkins, Mark
AU - Méndez-Callejas, Gina
AU - Oviedo, Jairo
AU - Guzmán, Fanny
AU - Patarroyo, Manuel A.
AU - Sánchez-Acedo, Caridad
AU - Quílez, Joaquín
N1 - Funding Information:
The mice were maintained according to Colombian Law 84/1989, Colombian Ministry of Health Resolution 8430/1993 Use of Laboratory Animals, 8th Edition (2011) (AVMA) Euthanasia panel on June 2007 was also taken into account [60,61] and the US Institutes of Health (NIH) Guide for the Care and [62] . That stipulated by the American Veterinary Medical Association’s [63] . The study was endorsed by Universidad de Ciencias Aplicadas y Ambientales (U.D.C.A) Research Ethics Committee, Minutes No. 17 (regulated by Agreement No. 285/2008, Chapter VII).
Publisher Copyright:
© 2018 Elsevier Ltd
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/10/29
Y1 - 2018/10/29
N2 - Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended.
AB - Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended.
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U2 - 10.1016/j.vaccine.2018.09.044
DO - 10.1016/j.vaccine.2018.09.044
M3 - Article
C2 - 30268732
AN - SCOPUS:85053915548
SN - 0264-410X
VL - 36
SP - 6703
EP - 6710
JO - Vaccine
JF - Vaccine
IS - 45
ER -
