Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides' immunogenicity and in vitro seroneutralisation capability

Catalina Avendaño, Mark Jenkins, Gina Méndez-Callejas, Jairo Oviedo, Fanny Guzmán, Manuel A Patarroyo, Caridad Sánchez-Acedo, Joaquín Quílez

Resultado de la investigación: Contribución a RevistaArtículo

Resumen

Cryptosporidium spp. es un protozoario zoonótico intracelular y una causa significativa de diarrea en humanos y animales en todo el mundo. Este parásito puede causar una alta morbilidad en personas inmunocomprometidas y niños en los países en desarrollo, siendo el ganado el principal reservorio. El objetivo de este estudio fue realizar pruebas preliminares en ratones suizos destetados albinos (ICR) para evaluar la respuesta inmune humoral inducida contra péptidos derivados de las proteínas CP15 (15 kDa antígeno de superficie de esporozoito de 15 kDa) y CSL (antígeno de tipo circunsporozoito). Los péptidos se identificaron y caracterizaron utilizando herramientas bioinformáticas y se sintetizaron químicamente. Se determinó la respuesta de los anticuerpos y se midió el efecto neutralizador de los anticuerpos en cultivo celular. A pesar de que todos los péptidos estudiados aquí fueron capaces de estimular la producción de anticuerpos, se detectaron anticuerpos neutralizantes para sólo dos de los derivados de CP15. Por lo tanto, se recomiendan estudios adicionales dirigidos a evaluar más a fondo el potencial de péptidos como candidatos para la vacuna.
Idioma originalEnglish (US)
Páginas (desde-hasta)6703-6710
Número de páginas8
PublicaciónVaccine
Volumen36
N.º45
DOI
EstadoE-pub ahead of print - sep 26 2018

Citar esto

Avendaño, Catalina ; Jenkins, Mark ; Méndez-Callejas, Gina ; Oviedo, Jairo ; Guzmán, Fanny ; Patarroyo, Manuel A ; Sánchez-Acedo, Caridad ; Quílez, Joaquín. / Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides' immunogenicity and in vitro seroneutralisation capability. En: Vaccine. 2018 ; Vol. 36, N.º 45. pp. 6703-6710.
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title = "Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides' immunogenicity and in vitro seroneutralisation capability",
abstract = "Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended.",
author = "Catalina Avenda{\~n}o and Mark Jenkins and Gina M{\'e}ndez-Callejas and Jairo Oviedo and Fanny Guzm{\'a}n and Patarroyo, {Manuel A} and Caridad S{\'a}nchez-Acedo and Joaqu{\'i}n Qu{\'i}lez",
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Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides' immunogenicity and in vitro seroneutralisation capability. / Avendaño, Catalina; Jenkins, Mark; Méndez-Callejas, Gina; Oviedo, Jairo; Guzmán, Fanny; Patarroyo, Manuel A; Sánchez-Acedo, Caridad; Quílez, Joaquín.

En: Vaccine, Vol. 36, N.º 45, 26.09.2018, p. 6703-6710.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides' immunogenicity and in vitro seroneutralisation capability

AU - Avendaño, Catalina

AU - Jenkins, Mark

AU - Méndez-Callejas, Gina

AU - Oviedo, Jairo

AU - Guzmán, Fanny

AU - Patarroyo, Manuel A

AU - Sánchez-Acedo, Caridad

AU - Quílez, Joaquín

N1 - Copyright © 2018 Elsevier Ltd. All rights reserved.

PY - 2018/9/26

Y1 - 2018/9/26

N2 - Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended.

AB - Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended.

U2 - 10.1016/j.vaccine.2018.09.044

DO - 10.1016/j.vaccine.2018.09.044

M3 - Article

C2 - 30268732

VL - 36

SP - 6703

EP - 6710

JO - Vaccine

JF - Vaccine

SN - 0264-410X

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ER -