Compositional properties of human cDNA libraries: Practical implications

Stilianos Arhondakis, Oliver Clay, Giorgio Bernardi

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

8 Citas (Scopus)

Resumen

The strikingly wide and bimodal gene distribution exhibited by the human genome has prompted us to study the correlations between EST-counts (expression levels) and base composition of genes, especially since existing data are contradictory. Here we investigate how cDNA library preparation affects the GC distributions of ESTs and/or genes found in the library, and address consequences for expression studies. We observe that strongly anomalous GC distributions often indicate experimental biases or deficits during their preparation. We propose the use of compositional distributions of raw ESTs from a cDNA library, and/or of the genes they represent, as a simple and effective tool for quality control.

Idioma originalInglés estadounidense
Páginas (desde-hasta)5772-5778
Número de páginas7
PublicaciónFEBS Letters
Volumen580
N.º24
DOI
EstadoPublicada - oct 16 2006
Publicado de forma externa

All Science Journal Classification (ASJC) codes

  • Biofísica
  • Biología estructural
  • Bioquímica
  • Biología molecular
  • Genética
  • Biología celular

Huella Profundice en los temas de investigación de 'Compositional properties of human cDNA libraries: Practical implications'. En conjunto forman una huella única.

Citar esto