Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes

Surendra Kumar Prajapati, Céline Borlon, Eduard Rovira-Vallbona, Jakub Gruszczyk, Sebastien Menant, Wai-Hong Tham, Johanna Helena Kattenberg, Elizabeth Villasis, Katlijn De Meulenaere, Dionicia Gamboa, Joseph Vinetz, Ricardo Fujita, Xa Nguyen Xuan, Marcelo Urbano Ferreira, Carlos H Niño, Manuel A Patarroyo, Gregory Spanakos, Luc Kestens, Jan Van Den Abbeele, Anna Rosanas-Urgell

Resultado de la investigación: Contribución a RevistaArtículo

1 Cita (Scopus)

Resumen

Plasmodium vivax parasites preferentially invade reticulocyte cells in a multistep process that is still poorly understood. In this study, we used ex vivo invasion assays and population genetic analyses to investigate the involvement of complement receptor 1 (CR1) in P. vivax invasion. First, we observed that P. vivax invasion of reticulocytes was consistently reduced when CR1 surface expression was reduced through enzymatic cleavage, in the presence of naturally low-CR1-expressing cells compared with high-CR1-expressing cells, and with the addition of soluble CR1, a known inhibitor of P. falciparum invasion. Immuno-precipitation experiments with P. vivax Reticulocyte Binding Proteins showed no evidence of complex formation. In addition, analysis of CR1 genetic data for worldwide human populations with different exposure to malaria parasites show significantly higher frequency of CR1 alleles associated with low receptor expression on the surface of RBCs and higher linkage disequilibrium in human populations exposed to P. vivax malaria compared with unexposed populations. These results are consistent with a positive selection of low-CR1-expressing alleles in vivax-endemic areas. Collectively, our findings demonstrate that CR1 availability on the surface of RBCs modulates P. vivax invasion. The identification of new molecular interactions is crucial to guiding the rational development of new therapeutic interventions against vivax malaria.

Idioma originalEnglish (US)
Páginas (desde-hasta)8943
PublicaciónScientific Reports
Volumen9
N.º1
DOI
EstadoPublished - jun 20 2019

Citar esto

Prajapati, S. K., Borlon, C., Rovira-Vallbona, E., Gruszczyk, J., Menant, S., Tham, W-H., ... Rosanas-Urgell, A. (2019). Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes. Scientific Reports, 9(1), 8943. https://doi.org/10.1038/s41598-019-45228-6
Prajapati, Surendra Kumar ; Borlon, Céline ; Rovira-Vallbona, Eduard ; Gruszczyk, Jakub ; Menant, Sebastien ; Tham, Wai-Hong ; Kattenberg, Johanna Helena ; Villasis, Elizabeth ; De Meulenaere, Katlijn ; Gamboa, Dionicia ; Vinetz, Joseph ; Fujita, Ricardo ; Xuan, Xa Nguyen ; Urbano Ferreira, Marcelo ; Niño, Carlos H ; Patarroyo, Manuel A ; Spanakos, Gregory ; Kestens, Luc ; Abbeele, Jan Van Den ; Rosanas-Urgell, Anna. / Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes. En: Scientific Reports. 2019 ; Vol. 9, N.º 1. pp. 8943.
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title = "Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes",
abstract = "Plasmodium vivax parasites preferentially invade reticulocyte cells in a multistep process that is still poorly understood. In this study, we used ex vivo invasion assays and population genetic analyses to investigate the involvement of complement receptor 1 (CR1) in P. vivax invasion. First, we observed that P. vivax invasion of reticulocytes was consistently reduced when CR1 surface expression was reduced through enzymatic cleavage, in the presence of naturally low-CR1-expressing cells compared with high-CR1-expressing cells, and with the addition of soluble CR1, a known inhibitor of P. falciparum invasion. Immuno-precipitation experiments with P. vivax Reticulocyte Binding Proteins showed no evidence of complex formation. In addition, analysis of CR1 genetic data for worldwide human populations with different exposure to malaria parasites show significantly higher frequency of CR1 alleles associated with low receptor expression on the surface of RBCs and higher linkage disequilibrium in human populations exposed to P. vivax malaria compared with unexposed populations. These results are consistent with a positive selection of low-CR1-expressing alleles in vivax-endemic areas. Collectively, our findings demonstrate that CR1 availability on the surface of RBCs modulates P. vivax invasion. The identification of new molecular interactions is crucial to guiding the rational development of new therapeutic interventions against vivax malaria.",
author = "Prajapati, {Surendra Kumar} and C{\'e}line Borlon and Eduard Rovira-Vallbona and Jakub Gruszczyk and Sebastien Menant and Wai-Hong Tham and Kattenberg, {Johanna Helena} and Elizabeth Villasis and {De Meulenaere}, Katlijn and Dionicia Gamboa and Joseph Vinetz and Ricardo Fujita and Xuan, {Xa Nguyen} and {Urbano Ferreira}, Marcelo and Ni{\~n}o, {Carlos H} and Patarroyo, {Manuel A} and Gregory Spanakos and Luc Kestens and Abbeele, {Jan Van Den} and Anna Rosanas-Urgell",
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month = "6",
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doi = "10.1038/s41598-019-45228-6",
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Prajapati, SK, Borlon, C, Rovira-Vallbona, E, Gruszczyk, J, Menant, S, Tham, W-H, Kattenberg, JH, Villasis, E, De Meulenaere, K, Gamboa, D, Vinetz, J, Fujita, R, Xuan, XN, Urbano Ferreira, M, Niño, CH, Patarroyo, MA, Spanakos, G, Kestens, L, Abbeele, JVD & Rosanas-Urgell, A 2019, 'Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes', Scientific Reports, vol. 9, n.º 1, pp. 8943. https://doi.org/10.1038/s41598-019-45228-6

Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes. / Prajapati, Surendra Kumar; Borlon, Céline; Rovira-Vallbona, Eduard; Gruszczyk, Jakub; Menant, Sebastien; Tham, Wai-Hong; Kattenberg, Johanna Helena; Villasis, Elizabeth; De Meulenaere, Katlijn; Gamboa, Dionicia; Vinetz, Joseph; Fujita, Ricardo; Xuan, Xa Nguyen; Urbano Ferreira, Marcelo; Niño, Carlos H; Patarroyo, Manuel A; Spanakos, Gregory; Kestens, Luc; Abbeele, Jan Van Den; Rosanas-Urgell, Anna.

En: Scientific Reports, Vol. 9, N.º 1, 20.06.2019, p. 8943.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes

AU - Prajapati, Surendra Kumar

AU - Borlon, Céline

AU - Rovira-Vallbona, Eduard

AU - Gruszczyk, Jakub

AU - Menant, Sebastien

AU - Tham, Wai-Hong

AU - Kattenberg, Johanna Helena

AU - Villasis, Elizabeth

AU - De Meulenaere, Katlijn

AU - Gamboa, Dionicia

AU - Vinetz, Joseph

AU - Fujita, Ricardo

AU - Xuan, Xa Nguyen

AU - Urbano Ferreira, Marcelo

AU - Niño, Carlos H

AU - Patarroyo, Manuel A

AU - Spanakos, Gregory

AU - Kestens, Luc

AU - Abbeele, Jan Van Den

AU - Rosanas-Urgell, Anna

PY - 2019/6/20

Y1 - 2019/6/20

N2 - Plasmodium vivax parasites preferentially invade reticulocyte cells in a multistep process that is still poorly understood. In this study, we used ex vivo invasion assays and population genetic analyses to investigate the involvement of complement receptor 1 (CR1) in P. vivax invasion. First, we observed that P. vivax invasion of reticulocytes was consistently reduced when CR1 surface expression was reduced through enzymatic cleavage, in the presence of naturally low-CR1-expressing cells compared with high-CR1-expressing cells, and with the addition of soluble CR1, a known inhibitor of P. falciparum invasion. Immuno-precipitation experiments with P. vivax Reticulocyte Binding Proteins showed no evidence of complex formation. In addition, analysis of CR1 genetic data for worldwide human populations with different exposure to malaria parasites show significantly higher frequency of CR1 alleles associated with low receptor expression on the surface of RBCs and higher linkage disequilibrium in human populations exposed to P. vivax malaria compared with unexposed populations. These results are consistent with a positive selection of low-CR1-expressing alleles in vivax-endemic areas. Collectively, our findings demonstrate that CR1 availability on the surface of RBCs modulates P. vivax invasion. The identification of new molecular interactions is crucial to guiding the rational development of new therapeutic interventions against vivax malaria.

AB - Plasmodium vivax parasites preferentially invade reticulocyte cells in a multistep process that is still poorly understood. In this study, we used ex vivo invasion assays and population genetic analyses to investigate the involvement of complement receptor 1 (CR1) in P. vivax invasion. First, we observed that P. vivax invasion of reticulocytes was consistently reduced when CR1 surface expression was reduced through enzymatic cleavage, in the presence of naturally low-CR1-expressing cells compared with high-CR1-expressing cells, and with the addition of soluble CR1, a known inhibitor of P. falciparum invasion. Immuno-precipitation experiments with P. vivax Reticulocyte Binding Proteins showed no evidence of complex formation. In addition, analysis of CR1 genetic data for worldwide human populations with different exposure to malaria parasites show significantly higher frequency of CR1 alleles associated with low receptor expression on the surface of RBCs and higher linkage disequilibrium in human populations exposed to P. vivax malaria compared with unexposed populations. These results are consistent with a positive selection of low-CR1-expressing alleles in vivax-endemic areas. Collectively, our findings demonstrate that CR1 availability on the surface of RBCs modulates P. vivax invasion. The identification of new molecular interactions is crucial to guiding the rational development of new therapeutic interventions against vivax malaria.

U2 - 10.1038/s41598-019-45228-6

DO - 10.1038/s41598-019-45228-6

M3 - Article

C2 - 31221984

VL - 9

SP - 8943

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

ER -