Comparison of healing of full-thickness skin wounds grafted with multidirectional or unidirectional autologous artificial dermis

differential delivery of healing biomarkers

Título traducido de la contribución: Comparación de la cicatrización de heridas cutáneas de espesor total injertadas con dermis artificial autóloga multidireccional o unidireccional: entrega diferencial de biomarcadores de curación

M R Fontanilla, S Casadiegos, R H Bustos, M A Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

2 Citas (Scopus)

Resumen

Las citoquinas, las quimiocinas y los factores de crecimiento y remodelación orquestan la cicatrización de heridas cuando se produce un daño en la piel. Durante las primeras etapas, cuando la herida aún está abierta, la detección y cuantificación de estos compuestos podría proporcionar biomarcadores de cicatrización de heridas cutáneas, lo que podría ayudar a completar el escenario proporcionado por los datos de seguimiento clínico y los análisis histológicos e histomorfométricos. Este trabajo evaluó y comparó la cicatrización de heridas de piel de espesor total injertadas con dermis artificial hecha con fibroblastos de piel autólogos y andamios de colágeno tipo I unidireccionales o multidireccionales para probar esta hipótesis. Se detectaron y cuantificaron biomarcadores de cicatrización en el medio de cultivo de la dermis artificial y exudados de las heridas injertadas. El seguimiento clínico de los animales y los análisis histológicos e histomorfométricos mostraron diferencias en la integración del injerto, el cierre de la herida y los parámetros histológicos e histomorfométricos. La cuantificación por resonancia plasmónica superficial de 13 biomarcadores curativos indicó secreción diferencial de la mayoría de los factores cuantificados en el medio de cultivo por la dermis artificial multidireccional y unidireccional. Además, hubo diferencias significativas entre la concentración de algunos de los factores analizados en los exudados de las heridas injertadas con la dermis artificial evaluada. Estos hallazgos sugieren que la entrega diferencial de biomarcadores de curación inducidos por la direccionalidad del andamiaje utilizado para producir la dermis multidireccional y unidireccional fue suficiente para crear dos microambientes de heridas cutáneas que determinaron un resultado diferente de curación. En general, los datos indican que la cicatrización de las heridas injertadas con dermis artificial multidireccional autóloga es mejor que la de las heridas injertadas con la unidireccional.
Idioma originalEnglish (US)
Páginas (desde-hasta)1014-1024
Número de páginas11
PublicaciónDrug Delivery and Translational Research
Volumen8
N.º5
DOI
EstadoPublished - oct 8 2018
Publicado de forma externa

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title = "Comparison of healing of full-thickness skin wounds grafted with multidirectional or unidirectional autologous artificial dermis: differential delivery of healing biomarkers",
abstract = "Cytokines, chemokines, and growth and remodeling factors orchestrate wound healing when skin damage occurs. During early stages, when the wound is still open, detection and quantification of these compounds might provide biomarkers of skin wound healing, which could aid to complete the scenario provided by clinical follow-up data and histological and histomorphometric analyses. This work assessed and compared the healing of full-thickness skin wounds grafted with artificial dermis made with autologous skin fibroblasts and unidirectional or multidirectional type I collagen scaffolds to test this hypothesis. Biomarkers of healing were detected and quantified in the culture medium of artificial dermis and exudates from the grafted wounds. Clinical follow-up of animals and histological and histomorphometric analysis showed differences in graft integration, wound closure, and histological and histomorphometric parameters. Surface plasmon resonance quantification of 13 healing biomarkers indicated differential secretion of most of the quantified factors in culture medium by the multidirectional and unidirectional artificial dermis. Also, there were significant differences between the concentration of some of the factors analyzed in the exudates of wounds grafted with the evaluated artificial dermis. These findings suggest that differential delivery of healing biomarkers induced by the directionality of the scaffold used to produce the multidirectional and unidirectional dermis was sufficient to create two skin wound microenvironments that determined a different outcome of healing. Overall, data indicate that healing of wounds grafted with multidirectional autologous artificial dermis is better than that of the wounds grafted with the unidirectional one.",
author = "Fontanilla, {M R} and S Casadiegos and Bustos, {R H} and Patarroyo, {M A}",
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Comparison of healing of full-thickness skin wounds grafted with multidirectional or unidirectional autologous artificial dermis : differential delivery of healing biomarkers. / Fontanilla, M R; Casadiegos, S; Bustos, R H; Patarroyo, M A.

En: Drug Delivery and Translational Research, Vol. 8, N.º 5, 08.10.2018, p. 1014-1024.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Comparison of healing of full-thickness skin wounds grafted with multidirectional or unidirectional autologous artificial dermis

T2 - differential delivery of healing biomarkers

AU - Fontanilla, M R

AU - Casadiegos, S

AU - Bustos, R H

AU - Patarroyo, M A

PY - 2018/10/8

Y1 - 2018/10/8

N2 - Cytokines, chemokines, and growth and remodeling factors orchestrate wound healing when skin damage occurs. During early stages, when the wound is still open, detection and quantification of these compounds might provide biomarkers of skin wound healing, which could aid to complete the scenario provided by clinical follow-up data and histological and histomorphometric analyses. This work assessed and compared the healing of full-thickness skin wounds grafted with artificial dermis made with autologous skin fibroblasts and unidirectional or multidirectional type I collagen scaffolds to test this hypothesis. Biomarkers of healing were detected and quantified in the culture medium of artificial dermis and exudates from the grafted wounds. Clinical follow-up of animals and histological and histomorphometric analysis showed differences in graft integration, wound closure, and histological and histomorphometric parameters. Surface plasmon resonance quantification of 13 healing biomarkers indicated differential secretion of most of the quantified factors in culture medium by the multidirectional and unidirectional artificial dermis. Also, there were significant differences between the concentration of some of the factors analyzed in the exudates of wounds grafted with the evaluated artificial dermis. These findings suggest that differential delivery of healing biomarkers induced by the directionality of the scaffold used to produce the multidirectional and unidirectional dermis was sufficient to create two skin wound microenvironments that determined a different outcome of healing. Overall, data indicate that healing of wounds grafted with multidirectional autologous artificial dermis is better than that of the wounds grafted with the unidirectional one.

AB - Cytokines, chemokines, and growth and remodeling factors orchestrate wound healing when skin damage occurs. During early stages, when the wound is still open, detection and quantification of these compounds might provide biomarkers of skin wound healing, which could aid to complete the scenario provided by clinical follow-up data and histological and histomorphometric analyses. This work assessed and compared the healing of full-thickness skin wounds grafted with artificial dermis made with autologous skin fibroblasts and unidirectional or multidirectional type I collagen scaffolds to test this hypothesis. Biomarkers of healing were detected and quantified in the culture medium of artificial dermis and exudates from the grafted wounds. Clinical follow-up of animals and histological and histomorphometric analysis showed differences in graft integration, wound closure, and histological and histomorphometric parameters. Surface plasmon resonance quantification of 13 healing biomarkers indicated differential secretion of most of the quantified factors in culture medium by the multidirectional and unidirectional artificial dermis. Also, there were significant differences between the concentration of some of the factors analyzed in the exudates of wounds grafted with the evaluated artificial dermis. These findings suggest that differential delivery of healing biomarkers induced by the directionality of the scaffold used to produce the multidirectional and unidirectional dermis was sufficient to create two skin wound microenvironments that determined a different outcome of healing. Overall, data indicate that healing of wounds grafted with multidirectional autologous artificial dermis is better than that of the wounds grafted with the unidirectional one.

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