Characterization of ROP18 alleles in human toxoplasmosis

Título traducido de la contribución: Caracterización de los alelos ROP18 en toxoplasmosis humana

Víctor Sánchez, Alejandra De-la-Torre, Jorge Enrique Gómez-Marín

Resultado de la investigación: Contribución a una revistaArtículo

30 Citas (Scopus)

Resumen


El papel del gen virulento polimorfismos ROP18 no se conoce en la toxoplasmosis humana. Se analizaron un total de 320 muestras clínicas. En muestras positivas para el gen ROP18, determinamos mediante una PCR específica de alelo, si los pacientes obtenían la secuencia de ROP18 positiva de inserción corriente arriba de la cepa Toxoplasma (cepa avirulenta de ratón) o la secuencia de inserción Rop18 negativa corriente de Toxoplasma (cepa virulenta de ratón). Diseñamos un ensayo ELISA para anticuerpos contra péptidos derivados de ROP18 de los tres linajes clonales principales de Toxoplasma. 20 muestras clínicas fueron de calidad para el análisis de alelos ROP18. En pacientes con toxoplasmosis ocular, una reacción inflamatoria más alta en el ojo se asoció con un resultado negativo de PCR para la región aguas arriba de ROP18. 23.3%, 33% y 16.6% de los sueros de individuos con toxoplasmosis ocular fueron positivos para péptidos derivados de ROP18 de tipo I, tipo II y tipo III, respectivamente, pero este ensayo se vio afectado por la reacción cruzada. La ausencia de la secuencia de inserción del promotor de Toxoplasma ROP18 en la toxoplasmosis ocular se correlacionó con la respuesta inflamatoria ocular grave. La determinación de anticuerpos contra la proteína ROP18 no fue útil para el serotipado en la toxoplasmosis humana
Idioma originalInglés estadounidense
Páginas (desde-hasta)463-469
Número de páginas7
PublicaciónParasitology International
Volumen63
N.º2
DOI
EstadoPublicada - abr 1 2014
Publicado de forma externa

Huella dactilar

Ocular Toxoplasmosis
Toxoplasmosis
Toxoplasma
Alleles
Serotyping
Polymerase Chain Reaction
Peptides
Antibodies
Insertional Mutagenesis
Cross Reactions
Genes
Enzyme-Linked Immunosorbent Assay
Serum
Proteins

Citar esto

Sánchez, Víctor ; De-la-Torre, Alejandra ; Gómez-Marín, Jorge Enrique. / Characterization of ROP18 alleles in human toxoplasmosis. En: Parasitology International. 2014 ; Vol. 63, N.º 2. pp. 463-469.
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abstract = "The role of the virulent gene ROP18 polymorphisms is not known in human toxoplasmosis. A total of 320 clinical samples were analyzed. In samples positive for ROP18 gene, we determined by an allele specific PCR, if patients got the upstream insertion positive ROP18 sequence Toxoplasma strain (mouse avirulent strain) or the upstream insertion negative ROP18 sequence Toxoplasma strain (mouse virulent strain). We designed an ELISA assay for antibodies against ROP18 derived peptides from the three major clonal lineages of Toxoplasma. 20 clinical samples were of quality for ROP18 allele analysis. In patients with ocular toxoplasmosis, a higher inflammatory reaction on eye was associated to a PCR negative result for the upstream region of ROP18. 23.3{\%}, 33{\%} and 16.6{\%} of serums from individuals with ocular toxoplasmosis were positive for type I, type II and type III ROP18 derived peptides, respectively but this assay was affected by cross reaction. The absence of Toxoplasma ROP18 promoter insertion sequence in ocular toxoplasmosis was correlated with severe ocular inflammatory response. Determination of antibodies against ROP18 protein was not useful for serotyping in human toxoplasmosis.",
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Characterization of ROP18 alleles in human toxoplasmosis. / Sánchez, Víctor; De-la-Torre, Alejandra; Gómez-Marín, Jorge Enrique.

En: Parasitology International, Vol. 63, N.º 2, 01.04.2014, p. 463-469.

Resultado de la investigación: Contribución a una revistaArtículo

TY - JOUR

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AU - De-la-Torre, Alejandra

AU - Gómez-Marín, Jorge Enrique

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N2 - The role of the virulent gene ROP18 polymorphisms is not known in human toxoplasmosis. A total of 320 clinical samples were analyzed. In samples positive for ROP18 gene, we determined by an allele specific PCR, if patients got the upstream insertion positive ROP18 sequence Toxoplasma strain (mouse avirulent strain) or the upstream insertion negative ROP18 sequence Toxoplasma strain (mouse virulent strain). We designed an ELISA assay for antibodies against ROP18 derived peptides from the three major clonal lineages of Toxoplasma. 20 clinical samples were of quality for ROP18 allele analysis. In patients with ocular toxoplasmosis, a higher inflammatory reaction on eye was associated to a PCR negative result for the upstream region of ROP18. 23.3%, 33% and 16.6% of serums from individuals with ocular toxoplasmosis were positive for type I, type II and type III ROP18 derived peptides, respectively but this assay was affected by cross reaction. The absence of Toxoplasma ROP18 promoter insertion sequence in ocular toxoplasmosis was correlated with severe ocular inflammatory response. Determination of antibodies against ROP18 protein was not useful for serotyping in human toxoplasmosis.

AB - The role of the virulent gene ROP18 polymorphisms is not known in human toxoplasmosis. A total of 320 clinical samples were analyzed. In samples positive for ROP18 gene, we determined by an allele specific PCR, if patients got the upstream insertion positive ROP18 sequence Toxoplasma strain (mouse avirulent strain) or the upstream insertion negative ROP18 sequence Toxoplasma strain (mouse virulent strain). We designed an ELISA assay for antibodies against ROP18 derived peptides from the three major clonal lineages of Toxoplasma. 20 clinical samples were of quality for ROP18 allele analysis. In patients with ocular toxoplasmosis, a higher inflammatory reaction on eye was associated to a PCR negative result for the upstream region of ROP18. 23.3%, 33% and 16.6% of serums from individuals with ocular toxoplasmosis were positive for type I, type II and type III ROP18 derived peptides, respectively but this assay was affected by cross reaction. The absence of Toxoplasma ROP18 promoter insertion sequence in ocular toxoplasmosis was correlated with severe ocular inflammatory response. Determination of antibodies against ROP18 protein was not useful for serotyping in human toxoplasmosis.

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