Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)

Carolina López, Carlos F. Suárez, Luis F. Cadavid, Manuel E. Patarroyo, Manuel A. Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

5 Citas (Scopus)

Resumen

Non-human primates belonging to the Aotus genus have been shown to be excellent experimental models for evaluating drugs and vaccine candidates against malaria and other human diseases. The immune system of this animal model must be characterised to assess whether the results obtained here can be extrapolated to humans. Class I and II major histocompatibility complex (MHC) proteins are amongst the most important molecules involved in response to pathogens; in spite of this, the techniques available for genotyping these molecules are usually expensive and/or time-consuming. Previous studies have reported MHC-DRB class II gene typing by microsatellite in Old World primates and humans, showing that such technique provides a fast, reliable and effective alternative to the commonly used ones. Based on this information, a microsatellite present in MHC-DRB intron 2 and its evolutionary patterns were identified in two Aotus species (A. vociferans and A. nancymaae ), as well as its potential for genotyping class II MHC-DRB in these primates. © 2014 López et al.
Idioma originalEnglish (US)
PublicaciónPLoS One
DOI
EstadoPublished - may 12 2014

Huella dactilar

Platyrrhini
Dichlororibofuranosylbenzimidazole
Aotus (Cebidae)
major histocompatibility complex
Major Histocompatibility Complex
Microsatellite Repeats
microsatellite repeats
Primates
genotyping
Molecules
Genotyping Techniques
Immune system
Pathogens
Introns
MHC Class II Genes
Animals
Vaccines
Genes
human diseases
malaria

Citar esto

López, Carolina ; Suárez, Carlos F. ; Cadavid, Luis F. ; Patarroyo, Manuel E. ; Patarroyo, Manuel A. / Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini). En: PLoS One. 2014.
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abstract = "Non-human primates belonging to the Aotus genus have been shown to be excellent experimental models for evaluating drugs and vaccine candidates against malaria and other human diseases. The immune system of this animal model must be characterised to assess whether the results obtained here can be extrapolated to humans. Class I and II major histocompatibility complex (MHC) proteins are amongst the most important molecules involved in response to pathogens; in spite of this, the techniques available for genotyping these molecules are usually expensive and/or time-consuming. Previous studies have reported MHC-DRB class II gene typing by microsatellite in Old World primates and humans, showing that such technique provides a fast, reliable and effective alternative to the commonly used ones. Based on this information, a microsatellite present in MHC-DRB intron 2 and its evolutionary patterns were identified in two Aotus species (A. vociferans and A. nancymaae ), as well as its potential for genotyping class II MHC-DRB in these primates. {\circledC} 2014 L{\'o}pez et al.",
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Characterising a microsatellite for DRB typing in Aotus vociferans and Aotus nancymaae (Platyrrhini). / López, Carolina; Suárez, Carlos F.; Cadavid, Luis F.; Patarroyo, Manuel E.; Patarroyo, Manuel A.

En: PLoS One, 12.05.2014.

Resultado de la investigación: Contribución a RevistaArtículo

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AB - Non-human primates belonging to the Aotus genus have been shown to be excellent experimental models for evaluating drugs and vaccine candidates against malaria and other human diseases. The immune system of this animal model must be characterised to assess whether the results obtained here can be extrapolated to humans. Class I and II major histocompatibility complex (MHC) proteins are amongst the most important molecules involved in response to pathogens; in spite of this, the techniques available for genotyping these molecules are usually expensive and/or time-consuming. Previous studies have reported MHC-DRB class II gene typing by microsatellite in Old World primates and humans, showing that such technique provides a fast, reliable and effective alternative to the commonly used ones. Based on this information, a microsatellite present in MHC-DRB intron 2 and its evolutionary patterns were identified in two Aotus species (A. vociferans and A. nancymaae ), as well as its potential for genotyping class II MHC-DRB in these primates. © 2014 López et al.

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