Characterising atypical Candida albicans clinical isolates from six third-level hospitals in Bogotá, Colombia

Título traducido de la contribución: Caracterización de aislamientos clínicos atípicos de Candida albicans desde seis hospitales de tercer nivel en Bogotá, Colombia.

Giovanni Rodríguez-Leguizamón, Alessandro Fiori, Luisa F. López, Beatriz L. Gómez, Claudia M. Parra-Giraldo, Arley Gómez-López, Carlos F. Suárez, Andrés Ceballos, Patrick Van Dijck, Manuel A. Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

8 Citas (Scopus)

Resumen

Las especies de cándida son los agentes patógenos fúngicos más frecuentes que causan la enfermedad nosocomial en un entorno hospitalario. Estas especies deben estar correctamente identificadas para garantizar que se tomen las medidas de control adecuadas y que se dé un tratamiento adecuado a cada especie. Candida albicans está causando la mayor carga de enfermedades fúngicas a nivel mundial; el desafío radica en diferenciarla de las especies atípicas, menores y relacionadas emergentes como Candida dubliniensis y Candida africana. El propósito de este estudio fue comparar la identificación basada en el MALDI-TOF MS con sistemas de identificación estándar utilizando un conjunto de aislamientos nosocomiales. nMETHODS: Once muestras nosocomiales fueron recolectadas de seis hospitales de tercer nivel en Bogotá, Colombia. Todas las muestras se identificaron combinando MALDI-TOF MS con caracteres morfológicos, asimilación de carbohidratos y marcadores moleculares (D1/D2 y HWP1).RESULTADOS: El presente trabajo describe la primera colección de aislados clínicos atípicos de la Candida colombiana; estos fueron identificados como Candida albicans/Candida africana por su perfil MALDI-TOF MS. Las características fenotípicas mostraron que eran incapaces de producir clamidiosporas, asimilar la trehalosa, la glucosamina, la N-acetil-glucosamina y apenas crecían a 42 °C, como sería de esperar para la Candida africana. La identificación molecular de la región D1/D2 del ARN ribosómico de subunidades grandes y de las secuencias de proteína 1 de la pared celular HWP1 de estas cepas aisladas fue consistente con las de Candida albicans. Los espectros de masa obtenidos por MALDI-TOF MS se analizaron mediante el escalado multidimensional (MDS) y el análisis de conglomerados, revelando diferencias entre los albicanos de Candida, Candida africana, Candida dubliniensis y dos grupos de aislados clínicos que se agruparon según el escenario clínico, uno de ellos claramente relacionado con C. albicans. \NCONCLUSIÓN: Este estudio destaca la importancia de utilizar MALDI-TOF MS en combinación con morfología, asimilación de sustratos y marcadores moleculares para caracterizar especies de C. albicans relacionadas con Candida albicans- y atípicas, superando así los métodos de identificación convencionales. Este es el primer informe de aislamientos de este tipo obtenidos en hospitales en Colombia; el enfoque seguido podría ser útil para recopilar conocimientos sobre epidemiología local que, a su vez, podrían tener un impacto en el manejo clínico. Los hallazgos resaltan la complejidad de distinguir entre los aislados típicos y atípicos de Candida albicans en los hospitales.
Idioma originalEnglish (US)
Páginas (desde-hasta)1-10
Número de páginas10
PublicaciónBMC Microbiology
Volumen15
N.º1
DOI
EstadoPublished - oct 5 2015

Huella dactilar

Colombia
Candida albicans
Candida
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Glucosamine
Trehalose
Ribosomal RNA
Mycoses
Cell Wall
Cluster Analysis
Epidemiology
Carbohydrates

Citar esto

Rodríguez-Leguizamón, Giovanni ; Fiori, Alessandro ; López, Luisa F. ; Gómez, Beatriz L. ; Parra-Giraldo, Claudia M. ; Gómez-López, Arley ; Suárez, Carlos F. ; Ceballos, Andrés ; Van Dijck, Patrick ; Patarroyo, Manuel A. / Characterising atypical Candida albicans clinical isolates from six third-level hospitals in Bogotá, Colombia. En: BMC Microbiology. 2015 ; Vol. 15, N.º 1. pp. 1-10.
@article{b71da18d0a32478bb00098464ca6ca68,
title = "Characterising atypical Candida albicans clinical isolates from six third-level hospitals in Bogot{\'a}, Colombia",
abstract = "Candida species are the most frequently found fungal pathogens causing nosocomial disease in a hospital setting. Such species must be correctly identified to ensure that appropriate control measures are taken and that suitable treatment is given for each species. Candida albicans is causing most fungal disease burden worldwide; the challenge lies in differentiating it from emerging atypical, minor and related species such as Candida dubliniensis and Candida africana. The purpose of this study was to compare identification based on MALDI-TOF MS to standard identification systems using a set of nosocomial isolates.\n\nMETHODS: Eleven nosocomial samples were collected from 6 third-level hospitals in Bogot{\'a}, Colombia. All the samples were identified by combining MALDI-TOF MS with morphological characters, carbohydrate assimilation and molecular markers (D1/D2 and HWP1).\n\nRESULTS: The present work describes the first collection of atypical Colombian Candida clinical isolates; these were identified as Candida albicans/Candida africana by their MALDI-TOF MS profile. Phenotypical characteristics showed that they were unable to produce chlamydospores, assimilate trehalose, glucosamine, N- acetyl-glucosamine and barely grew at 42 °C, as would be expected for Candida africana. The molecular identification of the D1/D2 region of large subunit ribosomal RNA and HWP1 hyphal cell wall protein 1 sequences from these isolates was consistent with those for Candida albicans. The mass spectra obtained by MALDI-TOF MS were analysed by multi-dimensional scaling (MDS) and cluster analysis, differences being revealed between Candida albicans, Candida africana, Candida dubliniensis reference spectra and two clinical isolate groups which clustered according to the clinical setting, one of them being clearly related to C. albicans.\n\nCONCLUSION: This study highlights the importance of using MALDI-TOF MS in combination with morphology, substrate assimilation and molecular markers for characterising Candida albicans-related and atypical C. albicans species, thereby overcoming conventional identification methods. This is the first report of hospital-obtained isolates of this type in Colombia; the approach followed might be useful for gathering knowledge regarding local epidemiology which could, in turn, have an impact on clinical management. The findings highlight the complexity of distinguishing between typical and atypical Candida albicans isolates in hospitals.",
author = "Giovanni Rodr{\'i}guez-Leguizam{\'o}n and Alessandro Fiori and L{\'o}pez, {Luisa F.} and G{\'o}mez, {Beatriz L.} and Parra-Giraldo, {Claudia M.} and Arley G{\'o}mez-L{\'o}pez and Su{\'a}rez, {Carlos F.} and Andr{\'e}s Ceballos and {Van Dijck}, Patrick and Patarroyo, {Manuel A.}",
year = "2015",
month = "10",
day = "5",
doi = "10.1186/s12866-015-0535-0",
language = "English (US)",
volume = "15",
pages = "1--10",
journal = "BMC Microbiology",
issn = "1471-2180",
publisher = "BioMed Central",
number = "1",

}

Rodríguez-Leguizamón, G, Fiori, A, López, LF, Gómez, BL, Parra-Giraldo, CM, Gómez-López, A, Suárez, CF, Ceballos, A, Van Dijck, P & Patarroyo, MA 2015, 'Characterising atypical Candida albicans clinical isolates from six third-level hospitals in Bogotá, Colombia', BMC Microbiology, vol. 15, n.º 1, pp. 1-10. https://doi.org/10.1186/s12866-015-0535-0

Characterising atypical Candida albicans clinical isolates from six third-level hospitals in Bogotá, Colombia. / Rodríguez-Leguizamón, Giovanni; Fiori, Alessandro; López, Luisa F.; Gómez, Beatriz L.; Parra-Giraldo, Claudia M.; Gómez-López, Arley; Suárez, Carlos F.; Ceballos, Andrés; Van Dijck, Patrick; Patarroyo, Manuel A.

En: BMC Microbiology, Vol. 15, N.º 1, 05.10.2015, p. 1-10.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Characterising atypical Candida albicans clinical isolates from six third-level hospitals in Bogotá, Colombia

AU - Rodríguez-Leguizamón, Giovanni

AU - Fiori, Alessandro

AU - López, Luisa F.

AU - Gómez, Beatriz L.

AU - Parra-Giraldo, Claudia M.

AU - Gómez-López, Arley

AU - Suárez, Carlos F.

AU - Ceballos, Andrés

AU - Van Dijck, Patrick

AU - Patarroyo, Manuel A.

PY - 2015/10/5

Y1 - 2015/10/5

N2 - Candida species are the most frequently found fungal pathogens causing nosocomial disease in a hospital setting. Such species must be correctly identified to ensure that appropriate control measures are taken and that suitable treatment is given for each species. Candida albicans is causing most fungal disease burden worldwide; the challenge lies in differentiating it from emerging atypical, minor and related species such as Candida dubliniensis and Candida africana. The purpose of this study was to compare identification based on MALDI-TOF MS to standard identification systems using a set of nosocomial isolates.\n\nMETHODS: Eleven nosocomial samples were collected from 6 third-level hospitals in Bogotá, Colombia. All the samples were identified by combining MALDI-TOF MS with morphological characters, carbohydrate assimilation and molecular markers (D1/D2 and HWP1).\n\nRESULTS: The present work describes the first collection of atypical Colombian Candida clinical isolates; these were identified as Candida albicans/Candida africana by their MALDI-TOF MS profile. Phenotypical characteristics showed that they were unable to produce chlamydospores, assimilate trehalose, glucosamine, N- acetyl-glucosamine and barely grew at 42 °C, as would be expected for Candida africana. The molecular identification of the D1/D2 region of large subunit ribosomal RNA and HWP1 hyphal cell wall protein 1 sequences from these isolates was consistent with those for Candida albicans. The mass spectra obtained by MALDI-TOF MS were analysed by multi-dimensional scaling (MDS) and cluster analysis, differences being revealed between Candida albicans, Candida africana, Candida dubliniensis reference spectra and two clinical isolate groups which clustered according to the clinical setting, one of them being clearly related to C. albicans.\n\nCONCLUSION: This study highlights the importance of using MALDI-TOF MS in combination with morphology, substrate assimilation and molecular markers for characterising Candida albicans-related and atypical C. albicans species, thereby overcoming conventional identification methods. This is the first report of hospital-obtained isolates of this type in Colombia; the approach followed might be useful for gathering knowledge regarding local epidemiology which could, in turn, have an impact on clinical management. The findings highlight the complexity of distinguishing between typical and atypical Candida albicans isolates in hospitals.

AB - Candida species are the most frequently found fungal pathogens causing nosocomial disease in a hospital setting. Such species must be correctly identified to ensure that appropriate control measures are taken and that suitable treatment is given for each species. Candida albicans is causing most fungal disease burden worldwide; the challenge lies in differentiating it from emerging atypical, minor and related species such as Candida dubliniensis and Candida africana. The purpose of this study was to compare identification based on MALDI-TOF MS to standard identification systems using a set of nosocomial isolates.\n\nMETHODS: Eleven nosocomial samples were collected from 6 third-level hospitals in Bogotá, Colombia. All the samples were identified by combining MALDI-TOF MS with morphological characters, carbohydrate assimilation and molecular markers (D1/D2 and HWP1).\n\nRESULTS: The present work describes the first collection of atypical Colombian Candida clinical isolates; these were identified as Candida albicans/Candida africana by their MALDI-TOF MS profile. Phenotypical characteristics showed that they were unable to produce chlamydospores, assimilate trehalose, glucosamine, N- acetyl-glucosamine and barely grew at 42 °C, as would be expected for Candida africana. The molecular identification of the D1/D2 region of large subunit ribosomal RNA and HWP1 hyphal cell wall protein 1 sequences from these isolates was consistent with those for Candida albicans. The mass spectra obtained by MALDI-TOF MS were analysed by multi-dimensional scaling (MDS) and cluster analysis, differences being revealed between Candida albicans, Candida africana, Candida dubliniensis reference spectra and two clinical isolate groups which clustered according to the clinical setting, one of them being clearly related to C. albicans.\n\nCONCLUSION: This study highlights the importance of using MALDI-TOF MS in combination with morphology, substrate assimilation and molecular markers for characterising Candida albicans-related and atypical C. albicans species, thereby overcoming conventional identification methods. This is the first report of hospital-obtained isolates of this type in Colombia; the approach followed might be useful for gathering knowledge regarding local epidemiology which could, in turn, have an impact on clinical management. The findings highlight the complexity of distinguishing between typical and atypical Candida albicans isolates in hospitals.

U2 - 10.1186/s12866-015-0535-0

DO - 10.1186/s12866-015-0535-0

M3 - Article

C2 - 26438104

VL - 15

SP - 1

EP - 10

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

IS - 1

ER -