Resumen
Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption.
| Idioma original | English (US) |
|---|---|
| Páginas (desde-hasta) | 3125-3130 |
| Número de páginas | 6 |
| Publicación | Epidemiology and Infection |
| Volumen | 145 |
| N.º | 15 |
| DOI | |
| Estado | Published - nov 2017 |
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Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption. / Olaya-Galán, N N; Corredor-Figueroa, A P; Guzmán-Garzón, T C; Ríos-Hernandez, K S; Salas-Cárdenas, S P; Patarroyo, M A; Gutierrez, M F.
En: Epidemiology and Infection, Vol. 145, N.º 15, 11.2017, p. 3125-3130.Resultado de la investigación: Contribución a Revista › Artículo
TY - JOUR
T1 - Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption
AU - Olaya-Galán, N N
AU - Corredor-Figueroa, A P
AU - Guzmán-Garzón, T C
AU - Ríos-Hernandez, K S
AU - Salas-Cárdenas, S P
AU - Patarroyo, M A
AU - Gutierrez, M F
PY - 2017/11
Y1 - 2017/11
N2 - Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption.
AB - Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption.
U2 - 10.1017/S0950268817002229
DO - 10.1017/S0950268817002229
M3 - Article
C2 - 28956522
VL - 145
SP - 3125
EP - 3130
JO - Epidemiology and Infection
JF - Epidemiology and Infection
SN - 0950-2688
IS - 15
ER -