Blastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestation

Rodolfo Daniel Casero, Florencia Mongi, Angie Sánchez, Juan David Ramírez

Resultado de la investigación: Contribución a RevistaArtículo

24 Citas (Scopus)

Resumen

© 2015.Blastocystis is a human common enteric protist that may colonize a large variety of non-human hosts linked to symptoms and diseases such as abdominal pain, constipation, diarrhea, urticaria, flatulence and irritable bowel syndrome (IBS). Blastocystis exhibits remarkable genetic diversity and multiple subtypes (STs) within the genus with no absolute associations with clinical symptomatology. Here we analyzed fecal samples from Argentinean patients (. n=. 270) belonging to symptomatic (urticaria and non-specific gastrointestinal symptoms, n=. 39) and asymptomatic control (. n=. 28). Those patients infected with Blastocystis (. n=. 67) were submitted for morphological analysis, DNA extraction, 18S PCR, sequencing and STs identification according to DNA barcoding. Blastocystis vacuolar forms were the predominant morphotype (75%), ameboid-like forms were evidenced in 1.5% of samples. Blastocystis ST3 was detected in 71.6% (. n=. 48), of which 71.4%, (. n=. 35) and 28.6% (. n=. 14) belonged to symptomatic and asymptomatic respectively. Other subtypes identified were ST1 (14.9%), ST6 (7.5%) and ST2 (5.9%). Blastocystis 18S barcoding evidenced in non-urticaria symptomatic patients and asymptomatic control group the presence of allele 134 (ST3) (. p
Idioma originalEnglish (US)
Páginas (desde-hasta)156-161
Número de páginas6
PublicaciónActa Tropica
DOI
EstadoPublished - ago 1 2015

Huella dactilar

Blastocystis
Urticaria
Flatulence
Irritable Bowel Syndrome
DNA
Constipation
Abdominal Pain
Diarrhea
Alleles
Polymerase Chain Reaction
Control Groups

Citar esto

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title = "Blastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestation",
abstract = "{\circledC} 2015.Blastocystis is a human common enteric protist that may colonize a large variety of non-human hosts linked to symptoms and diseases such as abdominal pain, constipation, diarrhea, urticaria, flatulence and irritable bowel syndrome (IBS). Blastocystis exhibits remarkable genetic diversity and multiple subtypes (STs) within the genus with no absolute associations with clinical symptomatology. Here we analyzed fecal samples from Argentinean patients (. n=. 270) belonging to symptomatic (urticaria and non-specific gastrointestinal symptoms, n=. 39) and asymptomatic control (. n=. 28). Those patients infected with Blastocystis (. n=. 67) were submitted for morphological analysis, DNA extraction, 18S PCR, sequencing and STs identification according to DNA barcoding. Blastocystis vacuolar forms were the predominant morphotype (75{\%}), ameboid-like forms were evidenced in 1.5{\%} of samples. Blastocystis ST3 was detected in 71.6{\%} (. n=. 48), of which 71.4{\%}, (. n=. 35) and 28.6{\%} (. n=. 14) belonged to symptomatic and asymptomatic respectively. Other subtypes identified were ST1 (14.9{\%}), ST6 (7.5{\%}) and ST2 (5.9{\%}). Blastocystis 18S barcoding evidenced in non-urticaria symptomatic patients and asymptomatic control group the presence of allele 134 (ST3) (. p",
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Blastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestation. / Casero, Rodolfo Daniel; Mongi, Florencia; Sánchez, Angie; Ramírez, Juan David.

En: Acta Tropica, 01.08.2015, p. 156-161.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Blastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestation

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AU - Mongi, Florencia

AU - Sánchez, Angie

AU - Ramírez, Juan David

PY - 2015/8/1

Y1 - 2015/8/1

N2 - © 2015.Blastocystis is a human common enteric protist that may colonize a large variety of non-human hosts linked to symptoms and diseases such as abdominal pain, constipation, diarrhea, urticaria, flatulence and irritable bowel syndrome (IBS). Blastocystis exhibits remarkable genetic diversity and multiple subtypes (STs) within the genus with no absolute associations with clinical symptomatology. Here we analyzed fecal samples from Argentinean patients (. n=. 270) belonging to symptomatic (urticaria and non-specific gastrointestinal symptoms, n=. 39) and asymptomatic control (. n=. 28). Those patients infected with Blastocystis (. n=. 67) were submitted for morphological analysis, DNA extraction, 18S PCR, sequencing and STs identification according to DNA barcoding. Blastocystis vacuolar forms were the predominant morphotype (75%), ameboid-like forms were evidenced in 1.5% of samples. Blastocystis ST3 was detected in 71.6% (. n=. 48), of which 71.4%, (. n=. 35) and 28.6% (. n=. 14) belonged to symptomatic and asymptomatic respectively. Other subtypes identified were ST1 (14.9%), ST6 (7.5%) and ST2 (5.9%). Blastocystis 18S barcoding evidenced in non-urticaria symptomatic patients and asymptomatic control group the presence of allele 134 (ST3) (. p

AB - © 2015.Blastocystis is a human common enteric protist that may colonize a large variety of non-human hosts linked to symptoms and diseases such as abdominal pain, constipation, diarrhea, urticaria, flatulence and irritable bowel syndrome (IBS). Blastocystis exhibits remarkable genetic diversity and multiple subtypes (STs) within the genus with no absolute associations with clinical symptomatology. Here we analyzed fecal samples from Argentinean patients (. n=. 270) belonging to symptomatic (urticaria and non-specific gastrointestinal symptoms, n=. 39) and asymptomatic control (. n=. 28). Those patients infected with Blastocystis (. n=. 67) were submitted for morphological analysis, DNA extraction, 18S PCR, sequencing and STs identification according to DNA barcoding. Blastocystis vacuolar forms were the predominant morphotype (75%), ameboid-like forms were evidenced in 1.5% of samples. Blastocystis ST3 was detected in 71.6% (. n=. 48), of which 71.4%, (. n=. 35) and 28.6% (. n=. 14) belonged to symptomatic and asymptomatic respectively. Other subtypes identified were ST1 (14.9%), ST6 (7.5%) and ST2 (5.9%). Blastocystis 18S barcoding evidenced in non-urticaria symptomatic patients and asymptomatic control group the presence of allele 134 (ST3) (. p

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