Behavior and abundance of Anopheles darlingi in communities living in the Colombian Amazon riverside

César Camilo Prado, Luis Antonio Alvarado-Cabrera, Paola Andrea Camargo-Ayala, Diego Garzón-Ospina, Milena Camargo, Sara Cecilia Soto-De León, Juan Ricardo Cubides, Carmen Teresa Celis-Giraldo, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

Resumen

In the past few years, relative frequencies of malaria parasite species in communities living in the Colombian Amazon riverside have changed, being Plasmodium vivax (61.4%) and Plasmodium malariae (43.8%) the most frequent. Given this epidemiological scenario, it is important to determine the species of anophelines involved in these parasites' transmission. This study was carried out in June 2016 in two indigenous communities living close to the tributaries of the Amazon River using protected human bait. The results of this study showed a total abundance of 1,085 mosquitos, of which 99.2% corresponded to Anopheles darlingi. Additionally, only two anopheline species were found, showing low diversity in the study areas. Molecular confirmation of some individuals was then followed by evolutionary analysis by using the COI gene. Nested PCR was used for identifying the three Plasmodium species circulating in the study areas. Of the two species collected in this study, 21.0% of the An. darlingi mosquitoes were infected with P. malariae, 21.9% with P. vivax and 10.3% with Plasmodium falciparum. It exhibited exophilic and exophagic behavior in both study areas, having marked differences regarding its abundance in each community (Tipisca first sampling 49.4%, Tipisca second sampling 39.6% and Doce de Octubre 10.9%). Interestingly, An. mattogrossensis infected by P. vivax was found for the first time in Colombia (in 50% of the four females collected). Analysis of An. darlingi COI gene diversity indicated a single population maintaining a high gene flow between the study areas. The An. darlingi behavior pattern found in both communities represents a risk factor for the region's inhabitants living/working near these sites. This highlights the need for vector control efforts such as the use of personal repellents and insecticides for use on cattle, which must be made available in order to reduce this Anopheline's abundance.

Idioma originalEnglish (US)
Páginas (desde-hasta)e0213335
PublicaciónPLoS One
Volumen14
N.º3
DOI
EstadoPublished - mar 7 2019

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Prado, C. C., Alvarado-Cabrera, L. A., Camargo-Ayala, P. A., Garzón-Ospina, D., Camargo, M., Soto-De León, S. C., ... Patarroyo, M. A. (2019). Behavior and abundance of Anopheles darlingi in communities living in the Colombian Amazon riverside. PLoS One, 14(3), e0213335. https://doi.org/10.1371/journal.pone.0213335
Prado, César Camilo ; Alvarado-Cabrera, Luis Antonio ; Camargo-Ayala, Paola Andrea ; Garzón-Ospina, Diego ; Camargo, Milena ; Soto-De León, Sara Cecilia ; Cubides, Juan Ricardo ; Celis-Giraldo, Carmen Teresa ; Patarroyo, Manuel Elkin ; Patarroyo, Manuel Alfonso. / Behavior and abundance of Anopheles darlingi in communities living in the Colombian Amazon riverside. En: PLoS One. 2019 ; Vol. 14, N.º 3. pp. e0213335.
@article{694cf93cba6f4cd59d938428f74150a2,
title = "Behavior and abundance of Anopheles darlingi in communities living in the Colombian Amazon riverside",
abstract = "In the past few years, relative frequencies of malaria parasite species in communities living in the Colombian Amazon riverside have changed, being Plasmodium vivax (61.4{\%}) and Plasmodium malariae (43.8{\%}) the most frequent. Given this epidemiological scenario, it is important to determine the species of anophelines involved in these parasites' transmission. This study was carried out in June 2016 in two indigenous communities living close to the tributaries of the Amazon River using protected human bait. The results of this study showed a total abundance of 1,085 mosquitos, of which 99.2{\%} corresponded to Anopheles darlingi. Additionally, only two anopheline species were found, showing low diversity in the study areas. Molecular confirmation of some individuals was then followed by evolutionary analysis by using the COI gene. Nested PCR was used for identifying the three Plasmodium species circulating in the study areas. Of the two species collected in this study, 21.0{\%} of the An. darlingi mosquitoes were infected with P. malariae, 21.9{\%} with P. vivax and 10.3{\%} with Plasmodium falciparum. It exhibited exophilic and exophagic behavior in both study areas, having marked differences regarding its abundance in each community (Tipisca first sampling 49.4{\%}, Tipisca second sampling 39.6{\%} and Doce de Octubre 10.9{\%}). Interestingly, An. mattogrossensis infected by P. vivax was found for the first time in Colombia (in 50{\%} of the four females collected). Analysis of An. darlingi COI gene diversity indicated a single population maintaining a high gene flow between the study areas. The An. darlingi behavior pattern found in both communities represents a risk factor for the region's inhabitants living/working near these sites. This highlights the need for vector control efforts such as the use of personal repellents and insecticides for use on cattle, which must be made available in order to reduce this Anopheline's abundance.",
author = "Prado, {C{\'e}sar Camilo} and Alvarado-Cabrera, {Luis Antonio} and Camargo-Ayala, {Paola Andrea} and Diego Garz{\'o}n-Ospina and Milena Camargo and {Soto-De Le{\'o}n}, {Sara Cecilia} and Cubides, {Juan Ricardo} and Celis-Giraldo, {Carmen Teresa} and Patarroyo, {Manuel Elkin} and Patarroyo, {Manuel Alfonso}",
year = "2019",
month = "3",
day = "7",
doi = "10.1371/journal.pone.0213335",
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Prado, CC, Alvarado-Cabrera, LA, Camargo-Ayala, PA, Garzón-Ospina, D, Camargo, M, Soto-De León, SC, Cubides, JR, Celis-Giraldo, CT, Patarroyo, ME & Patarroyo, MA 2019, 'Behavior and abundance of Anopheles darlingi in communities living in the Colombian Amazon riverside' PLoS One, vol. 14, n.º 3, pp. e0213335. https://doi.org/10.1371/journal.pone.0213335

Behavior and abundance of Anopheles darlingi in communities living in the Colombian Amazon riverside. / Prado, César Camilo; Alvarado-Cabrera, Luis Antonio; Camargo-Ayala, Paola Andrea; Garzón-Ospina, Diego; Camargo, Milena; Soto-De León, Sara Cecilia; Cubides, Juan Ricardo; Celis-Giraldo, Carmen Teresa; Patarroyo, Manuel Elkin; Patarroyo, Manuel Alfonso.

En: PLoS One, Vol. 14, N.º 3, 07.03.2019, p. e0213335.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Behavior and abundance of Anopheles darlingi in communities living in the Colombian Amazon riverside

AU - Prado, César Camilo

AU - Alvarado-Cabrera, Luis Antonio

AU - Camargo-Ayala, Paola Andrea

AU - Garzón-Ospina, Diego

AU - Camargo, Milena

AU - Soto-De León, Sara Cecilia

AU - Cubides, Juan Ricardo

AU - Celis-Giraldo, Carmen Teresa

AU - Patarroyo, Manuel Elkin

AU - Patarroyo, Manuel Alfonso

PY - 2019/3/7

Y1 - 2019/3/7

N2 - In the past few years, relative frequencies of malaria parasite species in communities living in the Colombian Amazon riverside have changed, being Plasmodium vivax (61.4%) and Plasmodium malariae (43.8%) the most frequent. Given this epidemiological scenario, it is important to determine the species of anophelines involved in these parasites' transmission. This study was carried out in June 2016 in two indigenous communities living close to the tributaries of the Amazon River using protected human bait. The results of this study showed a total abundance of 1,085 mosquitos, of which 99.2% corresponded to Anopheles darlingi. Additionally, only two anopheline species were found, showing low diversity in the study areas. Molecular confirmation of some individuals was then followed by evolutionary analysis by using the COI gene. Nested PCR was used for identifying the three Plasmodium species circulating in the study areas. Of the two species collected in this study, 21.0% of the An. darlingi mosquitoes were infected with P. malariae, 21.9% with P. vivax and 10.3% with Plasmodium falciparum. It exhibited exophilic and exophagic behavior in both study areas, having marked differences regarding its abundance in each community (Tipisca first sampling 49.4%, Tipisca second sampling 39.6% and Doce de Octubre 10.9%). Interestingly, An. mattogrossensis infected by P. vivax was found for the first time in Colombia (in 50% of the four females collected). Analysis of An. darlingi COI gene diversity indicated a single population maintaining a high gene flow between the study areas. The An. darlingi behavior pattern found in both communities represents a risk factor for the region's inhabitants living/working near these sites. This highlights the need for vector control efforts such as the use of personal repellents and insecticides for use on cattle, which must be made available in order to reduce this Anopheline's abundance.

AB - In the past few years, relative frequencies of malaria parasite species in communities living in the Colombian Amazon riverside have changed, being Plasmodium vivax (61.4%) and Plasmodium malariae (43.8%) the most frequent. Given this epidemiological scenario, it is important to determine the species of anophelines involved in these parasites' transmission. This study was carried out in June 2016 in two indigenous communities living close to the tributaries of the Amazon River using protected human bait. The results of this study showed a total abundance of 1,085 mosquitos, of which 99.2% corresponded to Anopheles darlingi. Additionally, only two anopheline species were found, showing low diversity in the study areas. Molecular confirmation of some individuals was then followed by evolutionary analysis by using the COI gene. Nested PCR was used for identifying the three Plasmodium species circulating in the study areas. Of the two species collected in this study, 21.0% of the An. darlingi mosquitoes were infected with P. malariae, 21.9% with P. vivax and 10.3% with Plasmodium falciparum. It exhibited exophilic and exophagic behavior in both study areas, having marked differences regarding its abundance in each community (Tipisca first sampling 49.4%, Tipisca second sampling 39.6% and Doce de Octubre 10.9%). Interestingly, An. mattogrossensis infected by P. vivax was found for the first time in Colombia (in 50% of the four females collected). Analysis of An. darlingi COI gene diversity indicated a single population maintaining a high gene flow between the study areas. The An. darlingi behavior pattern found in both communities represents a risk factor for the region's inhabitants living/working near these sites. This highlights the need for vector control efforts such as the use of personal repellents and insecticides for use on cattle, which must be made available in order to reduce this Anopheline's abundance.

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DO - 10.1371/journal.pone.0213335

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SN - 1932-6203

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Prado CC, Alvarado-Cabrera LA, Camargo-Ayala PA, Garzón-Ospina D, Camargo M, Soto-De León SC y otros. Behavior and abundance of Anopheles darlingi in communities living in the Colombian Amazon riverside. PLoS One. 2019 mar 7;14(3):e0213335. https://doi.org/10.1371/journal.pone.0213335