Assessment of antibacterial activity by fat bodies extracts and hemolymph derived from the blowfly Sarconesiopsis magellanica (Diptera: Calliphoridae)

Jennifher Góngora, Andrea Díaz-Roa, María Antonia Gaona, Jesús Cortés-Vecino, Felio Bello

Resultado de la investigación: Contribución a RevistaArtículo

3 Citas (Scopus)

Resumen

© 2014 ACIN. Published by Elsevier España, S.L.U. All rights reserved.Objective: This study aimed to evaluate the in vitro antibacterial activity of fat body and hemolymph extracts from Sarconesiopsis magellanica (Diptera: Calliphoridae) third-instar larvae, compared to the effect obtained using the same extracts but derived from Lucilia sericata. S. magellanica blowflies are considered important in forensic sciences due to their usefulness in determining the post mortem interval. This blowfly could be useful in larval therapy due to its necrophagous habits. Materials and methods: Fat body from larvae was removed by dissection, and hemolymph via decapitation and centrifugation of larval specimens. The antibacterial effect was tested against Staphylococcus aureus and Pseudomonas aeruginosa using two methods: agar diffusion and colony forming units (CFU/mL). Results: Hemolymph and fat body extracts derived from both L. sericata and S. magellanica were effective against S. aureus and P. aeruginosa, with no significant differences between blowfly species, although with some differences between the bacterial strains. Conclusions: The results obtained suggest that S. magellanica and L. sericata fat body and hemolymph extracts might have a similar antimicrobial activity against these microorganisms when used to treat infected wounds.
Idioma originalEnglish (US)
Páginas (desde-hasta)3-9
Número de páginas7
PublicaciónInfectio
DOI
EstadoPublished - ene 1 2015

Huella dactilar

Fat Body
Hemolymph
Diptera
Pseudomonas aeruginosa
Larva
Staphylococcus aureus
Forensic Sciences
Decapitation
Centrifugation
Habits
Agar
Dissection
Stem Cells
Wounds and Injuries
Therapeutics

Citar esto

Góngora, Jennifher ; Díaz-Roa, Andrea ; Gaona, María Antonia ; Cortés-Vecino, Jesús ; Bello, Felio. / Assessment of antibacterial activity by fat bodies extracts and hemolymph derived from the blowfly Sarconesiopsis magellanica (Diptera: Calliphoridae). En: Infectio. 2015 ; pp. 3-9.
@article{c69f90efe66c44dfba7f0b318123e207,
title = "Assessment of antibacterial activity by fat bodies extracts and hemolymph derived from the blowfly Sarconesiopsis magellanica (Diptera: Calliphoridae)",
abstract = "{\circledC} 2014 ACIN. Published by Elsevier Espa{\~n}a, S.L.U. All rights reserved.Objective: This study aimed to evaluate the in vitro antibacterial activity of fat body and hemolymph extracts from Sarconesiopsis magellanica (Diptera: Calliphoridae) third-instar larvae, compared to the effect obtained using the same extracts but derived from Lucilia sericata. S. magellanica blowflies are considered important in forensic sciences due to their usefulness in determining the post mortem interval. This blowfly could be useful in larval therapy due to its necrophagous habits. Materials and methods: Fat body from larvae was removed by dissection, and hemolymph via decapitation and centrifugation of larval specimens. The antibacterial effect was tested against Staphylococcus aureus and Pseudomonas aeruginosa using two methods: agar diffusion and colony forming units (CFU/mL). Results: Hemolymph and fat body extracts derived from both L. sericata and S. magellanica were effective against S. aureus and P. aeruginosa, with no significant differences between blowfly species, although with some differences between the bacterial strains. Conclusions: The results obtained suggest that S. magellanica and L. sericata fat body and hemolymph extracts might have a similar antimicrobial activity against these microorganisms when used to treat infected wounds.",
author = "Jennifher G{\'o}ngora and Andrea D{\'i}az-Roa and Gaona, {Mar{\'i}a Antonia} and Jes{\'u}s Cort{\'e}s-Vecino and Felio Bello",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/j.infect.2014.09.003",
language = "English (US)",
pages = "3--9",
journal = "Infectio",
issn = "0123-9392",
publisher = "Elsevier",

}

Assessment of antibacterial activity by fat bodies extracts and hemolymph derived from the blowfly Sarconesiopsis magellanica (Diptera: Calliphoridae). / Góngora, Jennifher; Díaz-Roa, Andrea; Gaona, María Antonia; Cortés-Vecino, Jesús; Bello, Felio.

En: Infectio, 01.01.2015, p. 3-9.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Assessment of antibacterial activity by fat bodies extracts and hemolymph derived from the blowfly Sarconesiopsis magellanica (Diptera: Calliphoridae)

AU - Góngora, Jennifher

AU - Díaz-Roa, Andrea

AU - Gaona, María Antonia

AU - Cortés-Vecino, Jesús

AU - Bello, Felio

PY - 2015/1/1

Y1 - 2015/1/1

N2 - © 2014 ACIN. Published by Elsevier España, S.L.U. All rights reserved.Objective: This study aimed to evaluate the in vitro antibacterial activity of fat body and hemolymph extracts from Sarconesiopsis magellanica (Diptera: Calliphoridae) third-instar larvae, compared to the effect obtained using the same extracts but derived from Lucilia sericata. S. magellanica blowflies are considered important in forensic sciences due to their usefulness in determining the post mortem interval. This blowfly could be useful in larval therapy due to its necrophagous habits. Materials and methods: Fat body from larvae was removed by dissection, and hemolymph via decapitation and centrifugation of larval specimens. The antibacterial effect was tested against Staphylococcus aureus and Pseudomonas aeruginosa using two methods: agar diffusion and colony forming units (CFU/mL). Results: Hemolymph and fat body extracts derived from both L. sericata and S. magellanica were effective against S. aureus and P. aeruginosa, with no significant differences between blowfly species, although with some differences between the bacterial strains. Conclusions: The results obtained suggest that S. magellanica and L. sericata fat body and hemolymph extracts might have a similar antimicrobial activity against these microorganisms when used to treat infected wounds.

AB - © 2014 ACIN. Published by Elsevier España, S.L.U. All rights reserved.Objective: This study aimed to evaluate the in vitro antibacterial activity of fat body and hemolymph extracts from Sarconesiopsis magellanica (Diptera: Calliphoridae) third-instar larvae, compared to the effect obtained using the same extracts but derived from Lucilia sericata. S. magellanica blowflies are considered important in forensic sciences due to their usefulness in determining the post mortem interval. This blowfly could be useful in larval therapy due to its necrophagous habits. Materials and methods: Fat body from larvae was removed by dissection, and hemolymph via decapitation and centrifugation of larval specimens. The antibacterial effect was tested against Staphylococcus aureus and Pseudomonas aeruginosa using two methods: agar diffusion and colony forming units (CFU/mL). Results: Hemolymph and fat body extracts derived from both L. sericata and S. magellanica were effective against S. aureus and P. aeruginosa, with no significant differences between blowfly species, although with some differences between the bacterial strains. Conclusions: The results obtained suggest that S. magellanica and L. sericata fat body and hemolymph extracts might have a similar antimicrobial activity against these microorganisms when used to treat infected wounds.

U2 - 10.1016/j.infect.2014.09.003

DO - 10.1016/j.infect.2014.09.003

M3 - Article

SP - 3

EP - 9

JO - Infectio

JF - Infectio

SN - 0123-9392

ER -