Aplication of authenticity criteria in mitochondrial studies on archaic bone remains from a prehispanic Muisca population

Nancy Patricia Jara, Mónica Díaz, Victoria Villegas, Clara López De Mesa, Diana Torres, Jaime Bernal, Alberto Gómez, Ignacio Briceño

Resultado de la investigación: Contribución a RevistaArtículo

2 Citas (Scopus)

Resumen

Introduction: Ancient DNA (aDNA)studies can support hypotheses regarding ancient populations; molecular studies can analyze the local population's genetic composition, minimizing biases introduced by later migrations, demographic expansions, mutations, and bottleneck effects. These analyses must be performed with special care because of the low DNA concentrations and contamination risk; therefore, it is necessary to establish protocols to guarantee the reproducibility and veracity of results. Objective: The present study aims to establish a protocol to obtain ancient DNA from 16 pre-Columbian bone samples found in an excavation performed in the area «Candelaria La Nueva» in Bogota, Colombia, dated in the period «Muisca Tardio». Methods: Four founder mitochondrial DNA Amerindian haplotypes were analyzed by high resolution restriction enzyme analyses, obtaining fragments between 121 and 186 base pairs (bp). Different analyses were performed following a strict control of authenticity criteria regarding laboratory conditions, including: positive and negative controls, reproducibility of results, and verification of particular characteristics present in ancient DNA. Results: Results obtained from the bone samples showed the exclusive presence of haplogroup A in the population studied. This data support the statement of the archaeologists of a single biological population in space and time. The distribution of this haplogroup in a 100% frequency supports the hypothesis of Chibcha genetic affiliation. Conclusion: The present study is a contribution to the study of genetic diversity in archaic American populations, allowing the integration of geographic and historic data with genetic characterization techniques associated with linguistic, ethnographic, and glottochronology patterns. Following the protocol proposed in the present study allows fulfilling authenticity criteria for ancient samples with the available techniques. © 2010 Universidad del Valle, Facultad de Salud.
Idioma originalEnglish (US)
Páginas (desde-hasta)306-314
Número de páginas9
PublicaciónColombia Medica
EstadoPublished - oct 1 2010

Huella dactilar

Bone and Bones
Reproducibility of Results
Population
DNA Contamination
Genetic Techniques
Restriction Mapping
Colombia
Population Genetics
Linguistics
Mitochondrial DNA
Base Pairing
Haplotypes
Demography
Mutation
Ancient DNA

Citar esto

Jara, N. P., Díaz, M., Villegas, V., De Mesa, C. L., Torres, D., Bernal, J., ... Briceño, I. (2010). Aplication of authenticity criteria in mitochondrial studies on archaic bone remains from a prehispanic Muisca population. Colombia Medica, 306-314.
Jara, Nancy Patricia ; Díaz, Mónica ; Villegas, Victoria ; De Mesa, Clara López ; Torres, Diana ; Bernal, Jaime ; Gómez, Alberto ; Briceño, Ignacio. / Aplication of authenticity criteria in mitochondrial studies on archaic bone remains from a prehispanic Muisca population. En: Colombia Medica. 2010 ; pp. 306-314.
@article{676b6fce2a1d451fba65e6ce2a6334a6,
title = "Aplication of authenticity criteria in mitochondrial studies on archaic bone remains from a prehispanic Muisca population",
abstract = "Introduction: Ancient DNA (aDNA)studies can support hypotheses regarding ancient populations; molecular studies can analyze the local population's genetic composition, minimizing biases introduced by later migrations, demographic expansions, mutations, and bottleneck effects. These analyses must be performed with special care because of the low DNA concentrations and contamination risk; therefore, it is necessary to establish protocols to guarantee the reproducibility and veracity of results. Objective: The present study aims to establish a protocol to obtain ancient DNA from 16 pre-Columbian bone samples found in an excavation performed in the area «Candelaria La Nueva» in Bogota, Colombia, dated in the period «Muisca Tardio». Methods: Four founder mitochondrial DNA Amerindian haplotypes were analyzed by high resolution restriction enzyme analyses, obtaining fragments between 121 and 186 base pairs (bp). Different analyses were performed following a strict control of authenticity criteria regarding laboratory conditions, including: positive and negative controls, reproducibility of results, and verification of particular characteristics present in ancient DNA. Results: Results obtained from the bone samples showed the exclusive presence of haplogroup A in the population studied. This data support the statement of the archaeologists of a single biological population in space and time. The distribution of this haplogroup in a 100{\%} frequency supports the hypothesis of Chibcha genetic affiliation. Conclusion: The present study is a contribution to the study of genetic diversity in archaic American populations, allowing the integration of geographic and historic data with genetic characterization techniques associated with linguistic, ethnographic, and glottochronology patterns. Following the protocol proposed in the present study allows fulfilling authenticity criteria for ancient samples with the available techniques. {\circledC} 2010 Universidad del Valle, Facultad de Salud.",
author = "Jara, {Nancy Patricia} and M{\'o}nica D{\'i}az and Victoria Villegas and {De Mesa}, {Clara L{\'o}pez} and Diana Torres and Jaime Bernal and Alberto G{\'o}mez and Ignacio Brice{\~n}o",
year = "2010",
month = "10",
day = "1",
language = "English (US)",
pages = "306--314",
journal = "Colombia Medica",
issn = "0120-8322",
publisher = "Universidad del Valle",

}

Jara, NP, Díaz, M, Villegas, V, De Mesa, CL, Torres, D, Bernal, J, Gómez, A & Briceño, I 2010, 'Aplication of authenticity criteria in mitochondrial studies on archaic bone remains from a prehispanic Muisca population', Colombia Medica, pp. 306-314.

Aplication of authenticity criteria in mitochondrial studies on archaic bone remains from a prehispanic Muisca population. / Jara, Nancy Patricia; Díaz, Mónica; Villegas, Victoria; De Mesa, Clara López; Torres, Diana; Bernal, Jaime; Gómez, Alberto; Briceño, Ignacio.

En: Colombia Medica, 01.10.2010, p. 306-314.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Aplication of authenticity criteria in mitochondrial studies on archaic bone remains from a prehispanic Muisca population

AU - Jara, Nancy Patricia

AU - Díaz, Mónica

AU - Villegas, Victoria

AU - De Mesa, Clara López

AU - Torres, Diana

AU - Bernal, Jaime

AU - Gómez, Alberto

AU - Briceño, Ignacio

PY - 2010/10/1

Y1 - 2010/10/1

N2 - Introduction: Ancient DNA (aDNA)studies can support hypotheses regarding ancient populations; molecular studies can analyze the local population's genetic composition, minimizing biases introduced by later migrations, demographic expansions, mutations, and bottleneck effects. These analyses must be performed with special care because of the low DNA concentrations and contamination risk; therefore, it is necessary to establish protocols to guarantee the reproducibility and veracity of results. Objective: The present study aims to establish a protocol to obtain ancient DNA from 16 pre-Columbian bone samples found in an excavation performed in the area «Candelaria La Nueva» in Bogota, Colombia, dated in the period «Muisca Tardio». Methods: Four founder mitochondrial DNA Amerindian haplotypes were analyzed by high resolution restriction enzyme analyses, obtaining fragments between 121 and 186 base pairs (bp). Different analyses were performed following a strict control of authenticity criteria regarding laboratory conditions, including: positive and negative controls, reproducibility of results, and verification of particular characteristics present in ancient DNA. Results: Results obtained from the bone samples showed the exclusive presence of haplogroup A in the population studied. This data support the statement of the archaeologists of a single biological population in space and time. The distribution of this haplogroup in a 100% frequency supports the hypothesis of Chibcha genetic affiliation. Conclusion: The present study is a contribution to the study of genetic diversity in archaic American populations, allowing the integration of geographic and historic data with genetic characterization techniques associated with linguistic, ethnographic, and glottochronology patterns. Following the protocol proposed in the present study allows fulfilling authenticity criteria for ancient samples with the available techniques. © 2010 Universidad del Valle, Facultad de Salud.

AB - Introduction: Ancient DNA (aDNA)studies can support hypotheses regarding ancient populations; molecular studies can analyze the local population's genetic composition, minimizing biases introduced by later migrations, demographic expansions, mutations, and bottleneck effects. These analyses must be performed with special care because of the low DNA concentrations and contamination risk; therefore, it is necessary to establish protocols to guarantee the reproducibility and veracity of results. Objective: The present study aims to establish a protocol to obtain ancient DNA from 16 pre-Columbian bone samples found in an excavation performed in the area «Candelaria La Nueva» in Bogota, Colombia, dated in the period «Muisca Tardio». Methods: Four founder mitochondrial DNA Amerindian haplotypes were analyzed by high resolution restriction enzyme analyses, obtaining fragments between 121 and 186 base pairs (bp). Different analyses were performed following a strict control of authenticity criteria regarding laboratory conditions, including: positive and negative controls, reproducibility of results, and verification of particular characteristics present in ancient DNA. Results: Results obtained from the bone samples showed the exclusive presence of haplogroup A in the population studied. This data support the statement of the archaeologists of a single biological population in space and time. The distribution of this haplogroup in a 100% frequency supports the hypothesis of Chibcha genetic affiliation. Conclusion: The present study is a contribution to the study of genetic diversity in archaic American populations, allowing the integration of geographic and historic data with genetic characterization techniques associated with linguistic, ethnographic, and glottochronology patterns. Following the protocol proposed in the present study allows fulfilling authenticity criteria for ancient samples with the available techniques. © 2010 Universidad del Valle, Facultad de Salud.

M3 - Article

SP - 306

EP - 314

JO - Colombia Medica

JF - Colombia Medica

SN - 0120-8322

ER -