TY - JOUR
T1 - Antigen presentation by a macrophage-like cell line persistently infected with respiratory syncytial virus
AU - Guerrero-Plata, Antonieta
AU - Ortega, Enrique
AU - Ortíz-Navarrete, Vianney
AU - Gómez, Beatríz
N1 - Funding Information:
The experiments involving the generation and assay of cytotoxic T cells were done in the laboratory of Prof. Peter J.M. Openshaw, Department of Respiratory Medicine, National Heart and Lung Institute, Imperial College School of Medicine at St Mary’s, London W2 1PG, UK. We thank Dr. Openshaw for receiving A.G.-P. for pre-doctoral training in his laboratory. This investigation was partially supported by grants from Consejo Nacional de Ciencia y Tecnologı́a (3112/M) and Dirección General de Asuntos del Personal Académico, UNAM (IN206400). A.G.-P. was supported by Consejo Nacional de Ciencia y Tecnologı́a (Scholarship 87905), Programa de Apoyo a Estudiantes de Posgrado (Scholarship 102325), Fundación UNAM, and Programa Universitario de Investigación en Salud. The authors thank Veronica Yakoleff for editing the manuscript.
PY - 2004/1
Y1 - 2004/1
N2 - Severe infection by the human respiratory syncytial virus (RSV) early in life is associated with subsequent recurrent airway disease presumably mediated by dysregulation of the local immune response. Dysfunction of the immune response may be related to impaired macrophage functions. We have previously reported that RSV persistence in a macrophage culture (MΦper) alters Fcγ receptors (FcγR)-mediated phagocytosis and the production of pro-inflammatory cytokines. Here, we determined whether the ability of macrophages to process and present antigens and to stimulate RSV-specific CD8+ T cells was altered in MΦper. We also examined the level of expression of MHC class I molecules in MΦper and the ability of these cells to present viral antigens to specific T lymphocytes. Our results showed that antigen processing and presentation were not altered by chronic RSV infection, and suggested that MΦper were able to stimulate RSV-specific CD8 + T lymphocytes.
AB - Severe infection by the human respiratory syncytial virus (RSV) early in life is associated with subsequent recurrent airway disease presumably mediated by dysregulation of the local immune response. Dysfunction of the immune response may be related to impaired macrophage functions. We have previously reported that RSV persistence in a macrophage culture (MΦper) alters Fcγ receptors (FcγR)-mediated phagocytosis and the production of pro-inflammatory cytokines. Here, we determined whether the ability of macrophages to process and present antigens and to stimulate RSV-specific CD8+ T cells was altered in MΦper. We also examined the level of expression of MHC class I molecules in MΦper and the ability of these cells to present viral antigens to specific T lymphocytes. Our results showed that antigen processing and presentation were not altered by chronic RSV infection, and suggested that MΦper were able to stimulate RSV-specific CD8 + T lymphocytes.
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U2 - 10.1016/j.virusres.2003.10.005
DO - 10.1016/j.virusres.2003.10.005
M3 - Research Article
C2 - 14687952
AN - SCOPUS:0346849663
SN - 0168-1702
VL - 99
SP - 95
EP - 100
JO - Virus Research
JF - Virus Research
IS - 1
ER -
