Anti-alpha-actinin antibodies are part of the anti-cell membrane antibody spectrum that characterize patients with lupus nephritis

Guillaume Seret, Felipe Cañas, Laurence Pougnet-Di Costanzo, Catherine Hanrotel-Saliou, Sandrine Jousse-Joulin, Yannick Le Meur, Alain Saraux, Antoine Valeri, Chaim Putterman, Pierre Youinou, Adriana Rojas-Villarraga, Juan Manuel Anaya, Yves Renaudineau

Resultado de la investigación: Contribución a RevistaArtículo

12 Citas (Scopus)

Resumen

Anti-membrane autoantibodies (MbA) have been reported in sera from patients with lupus nephritis (LN) but the targets of the MbA remain to be explored, which is the aim of the current study. Sera were collected from 40 patients with LN determined by renal biopsy, and from 30 systemic lupus erythematosus (SLE) patients without clinical evidence of LN. Thirty autoimmune disease control patients (rheumatoid arthritis, Sjögren's syndrome and systemic sclerosis), and 30 healthy controls were also included. Using flow cytometry, the presence of anti-MbA was explored revealing that IgG anti-MbA positivity was associated with LN (62.5% vs 13.3%) when compared to non-LN SLE patients, autoimmune disease patients (6.7%) and healthy controls (0%). Next, using purified plasma membrane fractions from human embryonic kidney (HEK) cells, the more prominent targets and their occurrence rates were located at 50 kDa, 60/65 kDa, 90 kDa, 110 kDa, 180 kDa and 220 kDa. Alpha-actinin (110 kDa) autoAb was characterized as a major target in LN patients positive for anti-MbA, and anti-MbA binding activity was reduced (36.9 ± 13.7%) in the presence of α-actinin. Laminin (200 kDa) was also characterized as a minor target, which was not the case for annexin A2 (36 kDa). Finally, anti-MbA IgG subclass analysis indicated a predominance of IgG2. In conclusion, IgG anti-MbA were detected at high levels in LN patients supporting a primary pathogenic role for anti-MbA and anti-MbA/α-actinin+ in LN that needs further research.
Idioma originalEnglish (US)
Páginas (desde-hasta)54-61
Número de páginas8
PublicaciónJournal of Autoimmunity
Volumen61
DOI
EstadoPublished - jul 1 2015
Publicado de forma externa

Huella dactilar

Actinin
Lupus Nephritis
Autoantibodies
Anti-Idiotypic Antibodies
Cell Membrane
Membranes
Antibodies
Systemic Lupus Erythematosus
Autoimmune Diseases
Immunoglobulin G
Annexin A2
Kidney
Nephritis
Systemic Scleroderma
Sjogren's Syndrome
Laminin
Serum
Rheumatoid Arthritis
Flow Cytometry

Citar esto

Seret, G., Cañas, F., Pougnet-Di Costanzo, L., Hanrotel-Saliou, C., Jousse-Joulin, S., Le Meur, Y., ... Renaudineau, Y. (2015). Anti-alpha-actinin antibodies are part of the anti-cell membrane antibody spectrum that characterize patients with lupus nephritis. Journal of Autoimmunity, 61, 54-61. https://doi.org/10.1016/j.jaut.2015.05.009
Seret, Guillaume ; Cañas, Felipe ; Pougnet-Di Costanzo, Laurence ; Hanrotel-Saliou, Catherine ; Jousse-Joulin, Sandrine ; Le Meur, Yannick ; Saraux, Alain ; Valeri, Antoine ; Putterman, Chaim ; Youinou, Pierre ; Rojas-Villarraga, Adriana ; Anaya, Juan Manuel ; Renaudineau, Yves. / Anti-alpha-actinin antibodies are part of the anti-cell membrane antibody spectrum that characterize patients with lupus nephritis. En: Journal of Autoimmunity. 2015 ; Vol. 61. pp. 54-61.
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title = "Anti-alpha-actinin antibodies are part of the anti-cell membrane antibody spectrum that characterize patients with lupus nephritis",
abstract = "Anti-membrane autoantibodies (MbA) have been reported in sera from patients with lupus nephritis (LN) but the targets of the MbA remain to be explored, which is the aim of the current study. Sera were collected from 40 patients with LN determined by renal biopsy, and from 30 systemic lupus erythematosus (SLE) patients without clinical evidence of LN. Thirty autoimmune disease control patients (rheumatoid arthritis, Sj{\"o}gren's syndrome and systemic sclerosis), and 30 healthy controls were also included. Using flow cytometry, the presence of anti-MbA was explored revealing that IgG anti-MbA positivity was associated with LN (62.5{\%} vs 13.3{\%}) when compared to non-LN SLE patients, autoimmune disease patients (6.7{\%}) and healthy controls (0{\%}). Next, using purified plasma membrane fractions from human embryonic kidney (HEK) cells, the more prominent targets and their occurrence rates were located at 50 kDa, 60/65 kDa, 90 kDa, 110 kDa, 180 kDa and 220 kDa. Alpha-actinin (110 kDa) autoAb was characterized as a major target in LN patients positive for anti-MbA, and anti-MbA binding activity was reduced (36.9 ± 13.7{\%}) in the presence of α-actinin. Laminin (200 kDa) was also characterized as a minor target, which was not the case for annexin A2 (36 kDa). Finally, anti-MbA IgG subclass analysis indicated a predominance of IgG2. In conclusion, IgG anti-MbA were detected at high levels in LN patients supporting a primary pathogenic role for anti-MbA and anti-MbA/α-actinin+ in LN that needs further research.",
author = "Guillaume Seret and Felipe Ca{\~n}as and {Pougnet-Di Costanzo}, Laurence and Catherine Hanrotel-Saliou and Sandrine Jousse-Joulin and {Le Meur}, Yannick and Alain Saraux and Antoine Valeri and Chaim Putterman and Pierre Youinou and Adriana Rojas-Villarraga and Anaya, {Juan Manuel} and Yves Renaudineau",
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Seret, G, Cañas, F, Pougnet-Di Costanzo, L, Hanrotel-Saliou, C, Jousse-Joulin, S, Le Meur, Y, Saraux, A, Valeri, A, Putterman, C, Youinou, P, Rojas-Villarraga, A, Anaya, JM & Renaudineau, Y 2015, 'Anti-alpha-actinin antibodies are part of the anti-cell membrane antibody spectrum that characterize patients with lupus nephritis', Journal of Autoimmunity, vol. 61, pp. 54-61. https://doi.org/10.1016/j.jaut.2015.05.009

Anti-alpha-actinin antibodies are part of the anti-cell membrane antibody spectrum that characterize patients with lupus nephritis. / Seret, Guillaume; Cañas, Felipe; Pougnet-Di Costanzo, Laurence; Hanrotel-Saliou, Catherine; Jousse-Joulin, Sandrine; Le Meur, Yannick; Saraux, Alain; Valeri, Antoine; Putterman, Chaim; Youinou, Pierre; Rojas-Villarraga, Adriana; Anaya, Juan Manuel; Renaudineau, Yves.

En: Journal of Autoimmunity, Vol. 61, 01.07.2015, p. 54-61.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Anti-alpha-actinin antibodies are part of the anti-cell membrane antibody spectrum that characterize patients with lupus nephritis

AU - Seret, Guillaume

AU - Cañas, Felipe

AU - Pougnet-Di Costanzo, Laurence

AU - Hanrotel-Saliou, Catherine

AU - Jousse-Joulin, Sandrine

AU - Le Meur, Yannick

AU - Saraux, Alain

AU - Valeri, Antoine

AU - Putterman, Chaim

AU - Youinou, Pierre

AU - Rojas-Villarraga, Adriana

AU - Anaya, Juan Manuel

AU - Renaudineau, Yves

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Anti-membrane autoantibodies (MbA) have been reported in sera from patients with lupus nephritis (LN) but the targets of the MbA remain to be explored, which is the aim of the current study. Sera were collected from 40 patients with LN determined by renal biopsy, and from 30 systemic lupus erythematosus (SLE) patients without clinical evidence of LN. Thirty autoimmune disease control patients (rheumatoid arthritis, Sjögren's syndrome and systemic sclerosis), and 30 healthy controls were also included. Using flow cytometry, the presence of anti-MbA was explored revealing that IgG anti-MbA positivity was associated with LN (62.5% vs 13.3%) when compared to non-LN SLE patients, autoimmune disease patients (6.7%) and healthy controls (0%). Next, using purified plasma membrane fractions from human embryonic kidney (HEK) cells, the more prominent targets and their occurrence rates were located at 50 kDa, 60/65 kDa, 90 kDa, 110 kDa, 180 kDa and 220 kDa. Alpha-actinin (110 kDa) autoAb was characterized as a major target in LN patients positive for anti-MbA, and anti-MbA binding activity was reduced (36.9 ± 13.7%) in the presence of α-actinin. Laminin (200 kDa) was also characterized as a minor target, which was not the case for annexin A2 (36 kDa). Finally, anti-MbA IgG subclass analysis indicated a predominance of IgG2. In conclusion, IgG anti-MbA were detected at high levels in LN patients supporting a primary pathogenic role for anti-MbA and anti-MbA/α-actinin+ in LN that needs further research.

AB - Anti-membrane autoantibodies (MbA) have been reported in sera from patients with lupus nephritis (LN) but the targets of the MbA remain to be explored, which is the aim of the current study. Sera were collected from 40 patients with LN determined by renal biopsy, and from 30 systemic lupus erythematosus (SLE) patients without clinical evidence of LN. Thirty autoimmune disease control patients (rheumatoid arthritis, Sjögren's syndrome and systemic sclerosis), and 30 healthy controls were also included. Using flow cytometry, the presence of anti-MbA was explored revealing that IgG anti-MbA positivity was associated with LN (62.5% vs 13.3%) when compared to non-LN SLE patients, autoimmune disease patients (6.7%) and healthy controls (0%). Next, using purified plasma membrane fractions from human embryonic kidney (HEK) cells, the more prominent targets and their occurrence rates were located at 50 kDa, 60/65 kDa, 90 kDa, 110 kDa, 180 kDa and 220 kDa. Alpha-actinin (110 kDa) autoAb was characterized as a major target in LN patients positive for anti-MbA, and anti-MbA binding activity was reduced (36.9 ± 13.7%) in the presence of α-actinin. Laminin (200 kDa) was also characterized as a minor target, which was not the case for annexin A2 (36 kDa). Finally, anti-MbA IgG subclass analysis indicated a predominance of IgG2. In conclusion, IgG anti-MbA were detected at high levels in LN patients supporting a primary pathogenic role for anti-MbA and anti-MbA/α-actinin+ in LN that needs further research.

U2 - 10.1016/j.jaut.2015.05.009

DO - 10.1016/j.jaut.2015.05.009

M3 - Article

VL - 61

SP - 54

EP - 61

JO - Journal of Autoimmunity

JF - Journal of Autoimmunity

SN - 0896-8411

ER -