Concordance analysis between different methodologies used for identification of oral isolates of Candida species in Colombia

Título traducido de la contribución: Análisis de concordancia entre diferentes metodologías utilizadas para la identificación de aislados orales de especies de Candida en Colombia

Alejandra Zuluaga, Karen Arango-Bustamante, Diego H. Cáceres, Zilpa Adriana Sánchez-Quitan, Verónica Velásquez, Beatriz L. Gómez, Claudia M. Parra-Giraldo, Natalia Maldonado, Luz E. Cano, Catalina De Bedout, Raul Rivera

Resultado de la investigación: Contribución a RevistaArtículo

Resumen

Antecedentes:
La determinación de las especies de levaduras es fundamental no sólo para un diagnóstico preciso sino también para establecer un tratamiento adecuado del paciente. Se realizó un estudio de concordancia de cinco metodologías para la identificación de especies de aislados orales de Candida en Colombia.

Métodos:
Sesenta y siete aislamientos de Candida fueron probados por; API® 20C-AUX, Vitek®2 Compact, Vitek®MS, Microflex® y una prueba molecular (PCR panfúngica y secuenciación). El costo comercial y el tiempo de procesamiento de las muestras se realizó mediante análisis gráfico.

Resultados:
Panfungal PCR diferenció 12 especies de métodos Candida, Vitek®MS y Microflex® identificando 9 especies, y los métodos API® 20C-AUX y Vitek®2 Compact identificando 8 especies cada uno. El Kappa ponderado (wK) mostró un alto acuerdo entre Panfungal PCR, Vitek®MS, Microflex® y API® 20C-AUX (wK 0,62-0,93). El wK que incluía el método Vitek®2 Compact presentaba concordancias moderadas o buenas en comparación con los otros métodos (wK 0,56-0,73). Las metodologías basadas en MALDI TOF MS requieren 4 minutos para generar resultados y el método Microflex® tiene el precio de venta más bajo.

Conclusión:
Los métodos evaluados mostraron una alta concordancia en sus resultados, siendo mayor para los métodos moleculares y las metodologías basadas en MALDI TOF. Estos últimos son más rápidos y baratos, y se presentan como alternativas prometedoras para la identificación rutinaria de especies de levaduras del género Candida.
Idioma originalEnglish (US)
Páginas (desde-hasta)160-167
Número de páginas8
PublicaciónColombia Medica
Volumen49
N.º3
EstadoPublished - jul 23 2018

Citar esto

Zuluaga, A., Arango-Bustamante, K., Cáceres, D. H., Sánchez-Quitan, Z. A., Velásquez, V., Gómez, B. L., ... Rivera, R. (2018). Concordance analysis between different methodologies used for identification of oral isolates of Candida species in Colombia. Colombia Medica, 49(3), 160-167.
Zuluaga, Alejandra ; Arango-Bustamante, Karen ; Cáceres, Diego H. ; Sánchez-Quitan, Zilpa Adriana ; Velásquez, Verónica ; Gómez, Beatriz L. ; Parra-Giraldo, Claudia M. ; Maldonado, Natalia ; Cano, Luz E. ; De Bedout, Catalina ; Rivera, Raul. / Concordance analysis between different methodologies used for identification of oral isolates of Candida species in Colombia. En: Colombia Medica. 2018 ; Vol. 49, N.º 3. pp. 160-167.
@article{1dfbb523757747688e760630c9034b70,
title = "Concordance analysis between different methodologies used for identification of oral isolates of Candida species in Colombia",
abstract = "Background:The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of Candida in Colombia.Methods:Sixty-seven Candida isolates were tested by; API{\circledR} 20C-AUX, Vitek{\circledR}2 Compact, Vitek{\circledR}MS, Microflex{\circledR} and a molecular test (panfungal PCR and sequencing). The commercial cost and processing time of the samples was done by graphical analysis.Results:Panfungal PCR differentiated 12 species of Candida, Vitek{\circledR}MS and Microflex{\circledR} methods identified 9 species, and API{\circledR} 20C-AUX and Vitek{\circledR}2 Compact methods identified 8 species each. Weighted Kappa (wK) showed a high agreement between Panfungal PCR, Vitek{\circledR}MS, Microflex{\circledR} and API{\circledR} 20C-AUX (wK 0.62-0.93). The wK that involved the Vitek{\circledR}2 Compact method presented moderate or good concordances compared with the other methods (wK 0.56-0.73). Methodologies based on MALDI TOF MS required 4 minutes to generate results and the Microflex{\circledR} method had the lowest selling price.Conclusion:The methods evaluated showed high concordance in their results, being higher for the molecular methods and the methodologies based on MALDI TOF. The latter are faster and cheaper, presenting as promising alternatives for the routine identification of yeast species of the genus Candida. ",
author = "Alejandra Zuluaga and Karen Arango-Bustamante and C{\'a}ceres, {Diego H.} and S{\'a}nchez-Quitan, {Zilpa Adriana} and Ver{\'o}nica Vel{\'a}squez and G{\'o}mez, {Beatriz L.} and Parra-Giraldo, {Claudia M.} and Natalia Maldonado and Cano, {Luz E.} and {De Bedout}, Catalina and Raul Rivera",
year = "2018",
month = "7",
day = "23",
language = "English (US)",
volume = "49",
pages = "160--167",
journal = "Colombia Medica",
issn = "0120-8322",
publisher = "Facultad de Salud de la Universidad del Valle",
number = "3",

}

Zuluaga, A, Arango-Bustamante, K, Cáceres, DH, Sánchez-Quitan, ZA, Velásquez, V, Gómez, BL, Parra-Giraldo, CM, Maldonado, N, Cano, LE, De Bedout, C & Rivera, R 2018, 'Concordance analysis between different methodologies used for identification of oral isolates of Candida species in Colombia', Colombia Medica, vol. 49, n.º 3, pp. 160-167.

Concordance analysis between different methodologies used for identification of oral isolates of Candida species in Colombia. / Zuluaga, Alejandra; Arango-Bustamante, Karen; Cáceres, Diego H.; Sánchez-Quitan, Zilpa Adriana; Velásquez, Verónica; Gómez, Beatriz L.; Parra-Giraldo, Claudia M.; Maldonado, Natalia; Cano, Luz E.; De Bedout, Catalina; Rivera, Raul.

En: Colombia Medica, Vol. 49, N.º 3, 23.07.2018, p. 160-167.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Concordance analysis between different methodologies used for identification of oral isolates of Candida species in Colombia

AU - Zuluaga, Alejandra

AU - Arango-Bustamante, Karen

AU - Cáceres, Diego H.

AU - Sánchez-Quitan, Zilpa Adriana

AU - Velásquez, Verónica

AU - Gómez, Beatriz L.

AU - Parra-Giraldo, Claudia M.

AU - Maldonado, Natalia

AU - Cano, Luz E.

AU - De Bedout, Catalina

AU - Rivera, Raul

PY - 2018/7/23

Y1 - 2018/7/23

N2 - Background:The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of Candida in Colombia.Methods:Sixty-seven Candida isolates were tested by; API® 20C-AUX, Vitek®2 Compact, Vitek®MS, Microflex® and a molecular test (panfungal PCR and sequencing). The commercial cost and processing time of the samples was done by graphical analysis.Results:Panfungal PCR differentiated 12 species of Candida, Vitek®MS and Microflex® methods identified 9 species, and API® 20C-AUX and Vitek®2 Compact methods identified 8 species each. Weighted Kappa (wK) showed a high agreement between Panfungal PCR, Vitek®MS, Microflex® and API® 20C-AUX (wK 0.62-0.93). The wK that involved the Vitek®2 Compact method presented moderate or good concordances compared with the other methods (wK 0.56-0.73). Methodologies based on MALDI TOF MS required 4 minutes to generate results and the Microflex® method had the lowest selling price.Conclusion:The methods evaluated showed high concordance in their results, being higher for the molecular methods and the methodologies based on MALDI TOF. The latter are faster and cheaper, presenting as promising alternatives for the routine identification of yeast species of the genus Candida. 

AB - Background:The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of Candida in Colombia.Methods:Sixty-seven Candida isolates were tested by; API® 20C-AUX, Vitek®2 Compact, Vitek®MS, Microflex® and a molecular test (panfungal PCR and sequencing). The commercial cost and processing time of the samples was done by graphical analysis.Results:Panfungal PCR differentiated 12 species of Candida, Vitek®MS and Microflex® methods identified 9 species, and API® 20C-AUX and Vitek®2 Compact methods identified 8 species each. Weighted Kappa (wK) showed a high agreement between Panfungal PCR, Vitek®MS, Microflex® and API® 20C-AUX (wK 0.62-0.93). The wK that involved the Vitek®2 Compact method presented moderate or good concordances compared with the other methods (wK 0.56-0.73). Methodologies based on MALDI TOF MS required 4 minutes to generate results and the Microflex® method had the lowest selling price.Conclusion:The methods evaluated showed high concordance in their results, being higher for the molecular methods and the methodologies based on MALDI TOF. The latter are faster and cheaper, presenting as promising alternatives for the routine identification of yeast species of the genus Candida. 

M3 - Article

VL - 49

SP - 160

EP - 167

JO - Colombia Medica

JF - Colombia Medica

SN - 0120-8322

IS - 3

ER -

Zuluaga A, Arango-Bustamante K, Cáceres DH, Sánchez-Quitan ZA, Velásquez V, Gómez BL y otros. Concordance analysis between different methodologies used for identification of oral isolates of Candida species in Colombia. Colombia Medica. 2018 jul 23;49(3):160-167.