Abnormality of Oct-1 DNA binding in T cells from Sjogren's syndrome patients

Eliezer Flescher, Norma Vela-Roch, Noriyoshi Ogawa, Toru Nakabayashi, Agustin Escalante, Juan Manuel Anaya, Howard Dang, Norman Talal

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

7 Citas (Scopus)

Resumen

Primary Sjogren's syndrome (SS) is an autoimmune rheumatic disease characterized by T cell hypoactivity. To understand the diminished T cell response to activation signals, we measured nucleoprotein DNA-binding activities regulating gene expression during T cell activation using the electrophoretic mobility shift assay. Peripheral blood lymphocytes from 9/19 SS patients were found to be defective in their ability to bind an octomer sequence (Oct-1). This Oct-1-binding phenotype remained stable in culture for up to 3 days prior to activation. This abnormality was not seen in resting T cells nor T cells from patients with systemic lupus erythematosus, rheumatoid arthritis (RA), or SS accompanied by RA. The SS Oct-1 DNA-binding abnormality correlated significantly with an inability of cells to exit the G(o)/G(i) cell cycle phase when stimulated in vitro. Importantly, nucleoprotein extracts showing decreased DNA-binding activity had normal amounts of Oct-1 proteins as determined by immunoprecipitation, implying a functional defect in the Oct-1 protein. Moreover, defective DNA binding was corrected by treatment with acid phosphatase.

Idioma originalInglés estadounidense
Páginas (desde-hasta)2006-2011
Número de páginas6
PublicaciónEuropean Journal of Immunology
Volumen26
N.º9
DOI
EstadoPublicada - 1996
Publicado de forma externa

All Science Journal Classification (ASJC) codes

  • Inmulogía y alergología
  • Inmunología

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