TY - JOUR
T1 - A DNA barcode for land plants
AU - Hollingsworth, Peter M.
AU - Forrest, Laura L.
AU - Spouge, John L.
AU - Hajibabaei, Mehrdad
AU - Ratnasingham, Sujeevan
AU - van der Bank, Michelle
AU - Chase, Mark W.
AU - Cowan, Robyn S.
AU - Erickson, David L.
AU - Fazekas, Aron J.
AU - Graham, Sean W.
AU - James, Karen E.
AU - Kim, Ki Joong
AU - John Kress, W.
AU - Schneider, Harald
AU - van AlphenStahl, Jonathan
AU - Barrett, Spencer C.H.
AU - van den Berg, Cassio
AU - Bogarin, Diego
AU - Burgess, Kevin S.
AU - Cameron, Kenneth M.
AU - Carine, Mark
AU - Chacón, Juliana
AU - Clark, Alexandra
AU - Clarkson, James J.
AU - Conrad, Ferozah
AU - Devey, Dion S.
AU - Ford, Caroline S.
AU - Hedderson, Terry A.J.
AU - Hollingsworth, Michelle L.
AU - Husband, Brian C.
AU - Kelly, Laura J.
AU - Kesanakurti, Prasad R.
AU - Kim, Jung Sung
AU - Kim, Young Dong
AU - Lahaye, Renaud
AU - Lee, Hae Lim
AU - Long, David G.
AU - Madriñán, Santiago
AU - Maurin, Olivier
AU - Meusnier, Isabelle
AU - Newmaster, Steven G.
AU - Park, Chong Wook
AU - Percy, Diana M.
AU - Petersen, Gitte
AU - Richardson, James E.
AU - Salazar, Gerardo A.
AU - Savolainen, Vincent
AU - Seberg, Ole
AU - Wilkinson, Michael J.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2009/8/4
Y1 - 2009/8/4
N2 - DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK-psbI spacer, and trnH-psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.
AB - DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK-psbI spacer, and trnH-psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.
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U2 - 10.1073/pnas.0905845106
DO - 10.1073/pnas.0905845106
M3 - Article
C2 - 19666622
AN - SCOPUS:69149084832
SN - 0027-8424
VL - 106
SP - 12794
EP - 12797
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 31
ER -