A B-lymphocyte binding peptide from BNRF1 induced antibodies inhibiting EBV-invasion of B-lymphocytes

Ramsés López; Mauricio Urquiza; Helena Patino; Jorge Suárez; Claudia Reyes; Manuel A. Patarroyo; Manuel E. Patarroyo

doi:10.1016/j.biochi.2005.04.009

A B-lymphocyte binding peptide from BNRF1 induced antibodies inhibiting EBV-invasion of B-lymphocytes

Título traducido de la contribución: Un péptido de unión a linfocitos B de BNRF1 induce anticuerpos inhibidores de la invasión de linfocitos B por el VEB

Ramsés López
, Mauricio Urquiza
, Helena Patino
, Jorge Suárez
, Claudia Reyes
, Manuel A. Patarroyo
, Manuel E. Patarroyo

Producción científica: Contribución a revista › Artículo de Investigación › revisión exhaustiva

1 Cita (Scopus)

Resumen

El virus de Epstein-Barr (VEB) infecta las células diana humanas principalmente a través de las interacciones gp350/220-CD21 y gp42-MHCII; sin embargo, se ha demostrado que estas interacciones son prescindibles para la invasión por el VEB de células susceptibles, lo que sugiere que otras proteínas víricas están implicadas en este proceso. Es probable que la proteína BNRF1/p140 del tegumento esté implicada en la invasión del VEB de las células diana, ya que los anticuerpos anti-p140 inhiben la infección de los linfocitos B por el VEB y existen pruebas de que parte de la proteína se localiza en la superficie del virus. Se sintetizaron sesenta y seis péptidos, que cubrían toda la secuencia BNRF1/p140, y se probaron en ensayos de unión a líneas celulares linfoblastoides. Los péptidos 11465 y 11521 se unieron con gran afinidad a las células Raji, Ramos y P3HR-1, pero no a los eritrocitos, mostrando un comportamiento de unión celular similar al del VEB. Estos dos péptidos indujeron anticuerpos que reconocían células vivas infectadas por el VEB. Curiosamente, el péptido-11521 (YVLQNAHQIACHFHSNGTDA) o los anticuerpos inducidos por este péptido inhibieron la unión del VEB a los linfocitos B, lo que sugiere que esta región p140 podría estar implicada en la interacción entre el VEB y los linfocitos B.

Título traducido de la contribución	Un péptido de unión a linfocitos B de BNRF1 induce anticuerpos inhibidores de la invasión de linfocitos B por el VEB
Idioma original	Inglés estadounidense
Páginas (desde-hasta)	985-992
Número de páginas	8
Publicación	Biochimie
Volumen	87
N.º	11
DOI	https://doi.org/10.1016/j.biochi.2005.04.009
Estado	Publicada - nov 1 2005

ODS de las Naciones Unidas

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ODS 3: Salud y bienestar

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Inmulogía y alergología

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@article{03cd7e64465b413aa7c81fc241750f3b,

title = "A B-lymphocyte binding peptide from BNRF1 induced antibodies inhibiting EBV-invasion of B-lymphocytes",

abstract = "Epstein-Barr virus (EBV) infects human target cells mainly through gp350/220-CD21 and gp42-MHCII interactions; however, it has been shown that these interactions are dispensable for EBV-invasion of susceptible cells, suggesting that other viral proteins are involved in this process. It is probable that tegument BNRF1/p140 protein is involved in EBV-invasion of target cells, since anti-p140 antibodies inhibit EBV-infection of B-lymphocytes and there is evidence that part of the protein is located on virus surface. Sixty-six peptides, covering the entire BNRF1/p140 sequence, were synthesised and tested in lymphoblastoid cell line binding assays. Peptides 11465 and 11521 bound with high affinity to Raji, Ramos and P3HR-1 cells but not to erythrocytes, showing cell-binding behaviour similar to EBV. These two peptides induced antibodies recognising live EBV-infected cells. Interestingly, peptide-11521 (YVLQNAHQIACHFHSNGTDA) or antibodies induced by this peptide inhibited EBV-binding to B-lymphocytes, suggesting that this p140-region could be involved in EBV and B-lymphocyte interaction. {\textcopyright} 2005 Elsevier SAS. All rights reserved.",

author = "Rams{\'e}s L{\'o}pez and Mauricio Urquiza and Helena Patino and Jorge Su{\'a}rez and Claudia Reyes and Patarroyo, \{Manuel A.\} and Patarroyo, \{Manuel E.\}",

year = "2005",

month = nov,

day = "1",

doi = "10.1016/j.biochi.2005.04.009",

language = "English (US)",

volume = "87",

pages = "985--992",

journal = "Biochimie",

issn = "0300-9084",

publisher = "Elsevier BV",

number = "11",

}

TY - JOUR

T1 - A B-lymphocyte binding peptide from BNRF1 induced antibodies inhibiting EBV-invasion of B-lymphocytes

AU - López, Ramsés

AU - Urquiza, Mauricio

AU - Patino, Helena

AU - Suárez, Jorge

AU - Reyes, Claudia

AU - Patarroyo, Manuel A.

AU - Patarroyo, Manuel E.

PY - 2005/11/1

Y1 - 2005/11/1

N2 - Epstein-Barr virus (EBV) infects human target cells mainly through gp350/220-CD21 and gp42-MHCII interactions; however, it has been shown that these interactions are dispensable for EBV-invasion of susceptible cells, suggesting that other viral proteins are involved in this process. It is probable that tegument BNRF1/p140 protein is involved in EBV-invasion of target cells, since anti-p140 antibodies inhibit EBV-infection of B-lymphocytes and there is evidence that part of the protein is located on virus surface. Sixty-six peptides, covering the entire BNRF1/p140 sequence, were synthesised and tested in lymphoblastoid cell line binding assays. Peptides 11465 and 11521 bound with high affinity to Raji, Ramos and P3HR-1 cells but not to erythrocytes, showing cell-binding behaviour similar to EBV. These two peptides induced antibodies recognising live EBV-infected cells. Interestingly, peptide-11521 (YVLQNAHQIACHFHSNGTDA) or antibodies induced by this peptide inhibited EBV-binding to B-lymphocytes, suggesting that this p140-region could be involved in EBV and B-lymphocyte interaction. © 2005 Elsevier SAS. All rights reserved.

AB - Epstein-Barr virus (EBV) infects human target cells mainly through gp350/220-CD21 and gp42-MHCII interactions; however, it has been shown that these interactions are dispensable for EBV-invasion of susceptible cells, suggesting that other viral proteins are involved in this process. It is probable that tegument BNRF1/p140 protein is involved in EBV-invasion of target cells, since anti-p140 antibodies inhibit EBV-infection of B-lymphocytes and there is evidence that part of the protein is located on virus surface. Sixty-six peptides, covering the entire BNRF1/p140 sequence, were synthesised and tested in lymphoblastoid cell line binding assays. Peptides 11465 and 11521 bound with high affinity to Raji, Ramos and P3HR-1 cells but not to erythrocytes, showing cell-binding behaviour similar to EBV. These two peptides induced antibodies recognising live EBV-infected cells. Interestingly, peptide-11521 (YVLQNAHQIACHFHSNGTDA) or antibodies induced by this peptide inhibited EBV-binding to B-lymphocytes, suggesting that this p140-region could be involved in EBV and B-lymphocyte interaction. © 2005 Elsevier SAS. All rights reserved.

U2 - 10.1016/j.biochi.2005.04.009

DO - 10.1016/j.biochi.2005.04.009

M3 - Research Article

SN - 0300-9084

VL - 87

SP - 985

EP - 992

JO - Biochimie

JF - Biochimie

IS - 11

ER -

A B-lymphocyte binding peptide from BNRF1 induced antibodies inhibiting EBV-invasion of B-lymphocytes

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