Actividad de la ß-galactosidasa como marcador de senescencia en cultivos primarios del epitelio superficial del ovario.

Magda Carolina Sanchez Corredor, S.R. Ramírez, Sandra Rondón-Lagos, Lilian Chuaire Noack, Milcíades Ibáñez-Pinilla

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

ß-galactosidase activity reflects the rate of cellular aging in vitro. Such activity was quantified at pH 6 in ovarian epithelial cells from 28 donors without a history of cancer, by the chemoluminiscent method. The cells were serially cultured until they achieved the state of permanent growth arrest. During the exponential growth phase, all cultures showed a similar pattern of growth and low ß-galactosidase activity. However, both in the onset of decrease replicative activity, as well as in the onset of the stationary phase, there was a significant rise in the enzyme activity. Our results showed that ß-galactosidase activity can be considered as a replicative senescence marker of the ovarian surface epithelium at pH 6.
Título traducido de la contribuciónβ-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium
Idioma originalEspañol (Colombia)
Páginas (desde-hasta)351 - 367
Número de páginas16
PublicaciónInvestigacion Clinica
Volumen51
N.º3
EstadoPublicada - sep 2010

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