The Wingate Anaerobic Test (WAnT) became one of the most convenient tests used to evaluate anaerobic capacity and the effectiveness of anaerobic training programs for a variety of power sports. However, its use and interpretation as an evaluative measurement are limited because there are few published reference values derived from large numbers of subjects in nonathletic populations. We present reference values for the WAnT in Colombian healthy adults (aged 20-80 years old). The sample comprised 1,873 subjects (64% men) from Cali, Colombia, who were recruited for the study between 2002 and 2012. The 30-second WAnT was performed on a Monark ergometer. The WAnT resistance was set at 0.075 kp·kg-1 body mass (BM). The mean absolute peak power (PP), relative PP normalized to the BM, and the fatigue index (FI%) were calculated using the LMS method (L [curve Box-Cox], M [curve median], and S [curve coefficient of variation]) and expressed as tabulated percentiles from 3 to 97 and as smoothed centile curves (p3, P10, P25, P50, P75, P90, P97). Mean ± SD values for the patients' anthropometric data were 38.1 ± 11.7 years of age, 72.7 ± 14.2 kg weight, 1.68 ± 0.09 m height, and 25.6 ± 4.2 body mass index. Our results show that mean absolute PP value, relative PP relative values normalized to BM, and FI were 527.4 ± 131.7 W, 7.6 ± 2.3 W·kg-1, and 29.0 ± 15.7%, respectively. Men performed better than women in terms of PP and FI values. Nevertheless, the mean PP decreased with age and sex. Age-specific PP and FI normative values among healthy Colombian adults are defined. A more specific set of reference values is useful for clinicians and researchers studying anaerobic capacity in healthy adults.