Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region

Fabio Brito-Santos, Luciana Trilles, Carolina Firacative, Wieland Meyer, Marília Martins Nishikawa, Angela Cristina Veríssimo Junqueira , Amanda Coutinho de Souza, Filipe Anibal Carvalho-Costa, Bodo Wanke, Márcia dos Samntos Lazéra

Research output: Contribution to conferencePoster

Abstract

Objective: Cryptococcosis is a life-threatening systemic mycosis affecting humans and animals worldwide. The disease is acquired by inhalation of infectious propagules of the Cryptococcus neoformans/C. gattii species complexes from the environment. Pioneering environmental studies on cryptococcosis in AIDS in Central Africa and in Brazil demonstrated that individuals living in contaminated dwellings are at risk to acquire cryptococcosis from indoor contamination. In the heart of the Amazon rainforest, a study revealed the occurrence of C. gattii VGII in dust samples from dwellings and wood poles in a remote human settlement in the Rio Negro Microregion, Amazon, Brazil. The high density of cryptococcal colonies suggested that the local inhabitants are daily exposed to the yeast. In this study, we disclosed new evidences of C. neoformans and C. gattii 7 out of 79 investigated human dwellings and two out of 10 wood poles in the Rio Negro Basin, Brazilian Amazon Methods: Forty-eight C. gattii and 18 C. neoformans isolates were subtyped using the ISHAM MLST consensus scheme Results: In one of the positive dwelling (An/P9) 200 CFU/g of dark brown colonies were found, and identified as C. gattii VGII and in another dwelling (Re/P15) 300 CFU/g of dark brown colonies were found and identified as C. gattii VGII and C. neoformans VNI. The most common C. gattii subtypes were ST7 (Vancouver Island Outbreak subtype VGIIb, 24/48) and ST20 (Vancouver Island Outbreak subtype VGIIa, 11/48). Amongst the 18 C. neoformans (VNI) isolates ST93 and ST5 were identified, showing a clonal population. Mating type analysis demonstrated all isolates were MATα. Conclusion: The identified MLST subtypes from the environmental isolates in the microregion of Rio Negro indicate domiciliary dust as well as wood poles as a potential source for human infection with different subtypes of C. gattii VGII and C. neoformans VNI MATα and confirmed the link between remote human settlements in the Rio Negro river basin and the Pacific North West outbreak of cryptococcosis.
Original languageEnglish (US)
PagesS84
Number of pages1
StatePublished - Jun 6 2018
Event20th Congress of the International Society for Human and Animal Mycology - Amsterdam, Netherlands
Duration: Jun 30 2018Jul 4 2018
https://www.isham2018.org/en/Home_10_6_12.html

Conference

Conference20th Congress of the International Society for Human and Animal Mycology
Abbreviated title20th Congress ISHAM
CountryNetherlands
CityAmsterdam
Period6/30/187/4/18
Internet address

Cite this

Brito-Santos, F., Trilles, L., Firacative, C., Meyer, W., Nishikawa, M. M., Veríssimo Junqueira , A. C., ... dos Samntos Lazéra, M. (2018). Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region. S84. Poster session presented at 20th Congress of the International Society for Human and Animal Mycology, Amsterdam, Netherlands.
Brito-Santos, Fabio ; Trilles, Luciana ; Firacative, Carolina ; Meyer, Wieland ; Nishikawa, Marília Martins ; Veríssimo Junqueira , Angela Cristina ; Coutinho de Souza, Amanda ; Carvalho-Costa, Filipe Anibal ; Wanke, Bodo ; dos Samntos Lazéra, Márcia . / Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region. Poster session presented at 20th Congress of the International Society for Human and Animal Mycology, Amsterdam, Netherlands.1 p.
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title = "Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region",
abstract = "Objective: Cryptococcosis is a life-threatening systemic mycosis affecting humans and animals worldwide. The disease is acquired by inhalation of infectious propagules of the Cryptococcus neoformans/C. gattii species complexes from the environment. Pioneering environmental studies on cryptococcosis in AIDS in Central Africa and in Brazil demonstrated that individuals living in contaminated dwellings are at risk to acquire cryptococcosis from indoor contamination. In the heart of the Amazon rainforest, a study revealed the occurrence of C. gattii VGII in dust samples from dwellings and wood poles in a remote human settlement in the Rio Negro Microregion, Amazon, Brazil. The high density of cryptococcal colonies suggested that the local inhabitants are daily exposed to the yeast. In this study, we disclosed new evidences of C. neoformans and C. gattii 7 out of 79 investigated human dwellings and two out of 10 wood poles in the Rio Negro Basin, Brazilian Amazon Methods: Forty-eight C. gattii and 18 C. neoformans isolates were subtyped using the ISHAM MLST consensus scheme Results: In one of the positive dwelling (An/P9) 200 CFU/g of dark brown colonies were found, and identified as C. gattii VGII and in another dwelling (Re/P15) 300 CFU/g of dark brown colonies were found and identified as C. gattii VGII and C. neoformans VNI. The most common C. gattii subtypes were ST7 (Vancouver Island Outbreak subtype VGIIb, 24/48) and ST20 (Vancouver Island Outbreak subtype VGIIa, 11/48). Amongst the 18 C. neoformans (VNI) isolates ST93 and ST5 were identified, showing a clonal population. Mating type analysis demonstrated all isolates were MATα. Conclusion: The identified MLST subtypes from the environmental isolates in the microregion of Rio Negro indicate domiciliary dust as well as wood poles as a potential source for human infection with different subtypes of C. gattii VGII and C. neoformans VNI MATα and confirmed the link between remote human settlements in the Rio Negro river basin and the Pacific North West outbreak of cryptococcosis.",
author = "Fabio Brito-Santos and Luciana Trilles and Carolina Firacative and Wieland Meyer and Nishikawa, {Mar{\'i}lia Martins} and {Ver{\'i}ssimo Junqueira}, {Angela Cristina} and {Coutinho de Souza}, Amanda and Carvalho-Costa, {Filipe Anibal} and Bodo Wanke and {dos Samntos Laz{\'e}ra}, M{\'a}rcia",
year = "2018",
month = "6",
day = "6",
language = "English (US)",
pages = "S84",
note = "20th Congress of the International Society for Human and Animal Mycology, 20th Congress ISHAM ; Conference date: 30-06-2018 Through 04-07-2018",
url = "https://www.isham2018.org/en/Home_10_6_12.html",

}

Brito-Santos, F, Trilles, L, Firacative, C, Meyer, W, Nishikawa, MM, Veríssimo Junqueira , AC, Coutinho de Souza, A, Carvalho-Costa, FA, Wanke, B & dos Samntos Lazéra, M 2018, 'Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region' 20th Congress of the International Society for Human and Animal Mycology, Amsterdam, Netherlands, 6/30/18 - 7/4/18, pp. S84.

Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region. / Brito-Santos, Fabio; Trilles, Luciana; Firacative, Carolina; Meyer, Wieland; Nishikawa, Marília Martins; Veríssimo Junqueira , Angela Cristina; Coutinho de Souza, Amanda ; Carvalho-Costa, Filipe Anibal; Wanke, Bodo ; dos Samntos Lazéra, Márcia .

2018. S84 Poster session presented at 20th Congress of the International Society for Human and Animal Mycology, Amsterdam, Netherlands.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region

AU - Brito-Santos, Fabio

AU - Trilles, Luciana

AU - Firacative, Carolina

AU - Meyer, Wieland

AU - Nishikawa, Marília Martins

AU - Veríssimo Junqueira , Angela Cristina

AU - Coutinho de Souza, Amanda

AU - Carvalho-Costa, Filipe Anibal

AU - Wanke, Bodo

AU - dos Samntos Lazéra, Márcia

PY - 2018/6/6

Y1 - 2018/6/6

N2 - Objective: Cryptococcosis is a life-threatening systemic mycosis affecting humans and animals worldwide. The disease is acquired by inhalation of infectious propagules of the Cryptococcus neoformans/C. gattii species complexes from the environment. Pioneering environmental studies on cryptococcosis in AIDS in Central Africa and in Brazil demonstrated that individuals living in contaminated dwellings are at risk to acquire cryptococcosis from indoor contamination. In the heart of the Amazon rainforest, a study revealed the occurrence of C. gattii VGII in dust samples from dwellings and wood poles in a remote human settlement in the Rio Negro Microregion, Amazon, Brazil. The high density of cryptococcal colonies suggested that the local inhabitants are daily exposed to the yeast. In this study, we disclosed new evidences of C. neoformans and C. gattii 7 out of 79 investigated human dwellings and two out of 10 wood poles in the Rio Negro Basin, Brazilian Amazon Methods: Forty-eight C. gattii and 18 C. neoformans isolates were subtyped using the ISHAM MLST consensus scheme Results: In one of the positive dwelling (An/P9) 200 CFU/g of dark brown colonies were found, and identified as C. gattii VGII and in another dwelling (Re/P15) 300 CFU/g of dark brown colonies were found and identified as C. gattii VGII and C. neoformans VNI. The most common C. gattii subtypes were ST7 (Vancouver Island Outbreak subtype VGIIb, 24/48) and ST20 (Vancouver Island Outbreak subtype VGIIa, 11/48). Amongst the 18 C. neoformans (VNI) isolates ST93 and ST5 were identified, showing a clonal population. Mating type analysis demonstrated all isolates were MATα. Conclusion: The identified MLST subtypes from the environmental isolates in the microregion of Rio Negro indicate domiciliary dust as well as wood poles as a potential source for human infection with different subtypes of C. gattii VGII and C. neoformans VNI MATα and confirmed the link between remote human settlements in the Rio Negro river basin and the Pacific North West outbreak of cryptococcosis.

AB - Objective: Cryptococcosis is a life-threatening systemic mycosis affecting humans and animals worldwide. The disease is acquired by inhalation of infectious propagules of the Cryptococcus neoformans/C. gattii species complexes from the environment. Pioneering environmental studies on cryptococcosis in AIDS in Central Africa and in Brazil demonstrated that individuals living in contaminated dwellings are at risk to acquire cryptococcosis from indoor contamination. In the heart of the Amazon rainforest, a study revealed the occurrence of C. gattii VGII in dust samples from dwellings and wood poles in a remote human settlement in the Rio Negro Microregion, Amazon, Brazil. The high density of cryptococcal colonies suggested that the local inhabitants are daily exposed to the yeast. In this study, we disclosed new evidences of C. neoformans and C. gattii 7 out of 79 investigated human dwellings and two out of 10 wood poles in the Rio Negro Basin, Brazilian Amazon Methods: Forty-eight C. gattii and 18 C. neoformans isolates were subtyped using the ISHAM MLST consensus scheme Results: In one of the positive dwelling (An/P9) 200 CFU/g of dark brown colonies were found, and identified as C. gattii VGII and in another dwelling (Re/P15) 300 CFU/g of dark brown colonies were found and identified as C. gattii VGII and C. neoformans VNI. The most common C. gattii subtypes were ST7 (Vancouver Island Outbreak subtype VGIIb, 24/48) and ST20 (Vancouver Island Outbreak subtype VGIIa, 11/48). Amongst the 18 C. neoformans (VNI) isolates ST93 and ST5 were identified, showing a clonal population. Mating type analysis demonstrated all isolates were MATα. Conclusion: The identified MLST subtypes from the environmental isolates in the microregion of Rio Negro indicate domiciliary dust as well as wood poles as a potential source for human infection with different subtypes of C. gattii VGII and C. neoformans VNI MATα and confirmed the link between remote human settlements in the Rio Negro river basin and the Pacific North West outbreak of cryptococcosis.

M3 - Poster

SP - S84

ER -

Brito-Santos F, Trilles L, Firacative C, Meyer W, Nishikawa MM, Veríssimo Junqueira AC et al. Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region. 2018. Poster session presented at 20th Congress of the International Society for Human and Animal Mycology, Amsterdam, Netherlands.