Abstract
The identification of microorganisms in tissue is pivotal to recognize infectious processes. At first, the hematoxylin-eosin stain is used to identify the pattern of inflammation associated; after that, microorganisms are seen through Gram or silver stains. Gram Stain of tissue biopsy not only stains bacteria, but also a number of fungus and parasites. However, this technique has some disadvantages, such as contamination with other microorganisms, and lack of stain of some bacteria, including Legionella pneumophila, Leptospira spp and Bartonella spp. This review article aims to describe the fundamentals of Gram stain of tissue biopsy and its assistance in infectious diagnosis as an additional tool for recognition of microorganisms, as well as its limitations.
| Translated title of the contribution | Gram stain of tissue biopsy: scope and pitfalls |
|---|---|
| Original language | Spanish (Colombia) |
| Pages (from-to) | 557-573 |
| Number of pages | 17 |
| Journal | Medicina & laboratorio |
| Volume | 18 |
| Issue number | 11-12 |
| State | Published - Dec 2012 |
| Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine