The identification of two Trypanosoma cruzi I genotypes from domestic and sylvatic transmission cycles in Colombia based on a single polymerase chain reaction amplification of the spliced-leader intergenic region

Lina Marcela Villa, Felipe Guhl, Daniel Zabala, Juan David Ramírez, Daniel Alfonso Urrea, Diana Carolina Hernández, Zulma Cucunubá, Marleny Montilla, Julio César Carranza, Karina Rueda, Jorge Eduardo Trujillo, Gustavo Adolfo Vallejo

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A single polymerase chain reaction (PCR) reaction targeting the spliced-leader intergenic region of Trypanosoma cruzi I was standardised by amplifying a 231 bp fragment in domestic (TcIDOM) strains or clones and 450 and 550 bp fragments in sylvatic strains or clones. This reaction was validated using 44 blind coded samples and 184 non-coded T. cruzi I clones isolated from sylvatic triatomines and the correspondence between the amplified fragments and their domestic or sylvatic origin was determined. Six of the nine strains isolated from acute cases suspected of oral infection had the sylvatic T. cruzi I profile. These results confirmed that the sylvatic T. cruzi I genotype is linked to cases of oral Chagas disease in Colombia. We therefore propose the use of this novel PCR reaction in strains or clones previously characterised as T. cruzi I to distinguish TcIDOM from sylvatic genotypes in studies of transmission dynamics, including the verification of population selection within hosts or detection of the frequency of mixed infections by both T. cruzi I genotypes in Colombia.

Original languageEnglish (US)
Pages (from-to)932-935
Number of pages4
JournalMemorias do Instituto Oswaldo Cruz
Volume108
Issue number7
DOIs
StatePublished - Nov 2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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