The diagnostic performance of classical molecular tests used for detecting human papillomavirus

Marina Munoz, Milena Camargo, Sara C. Soto-De Leon, Adriana Rojas-Villarraga, Ricardo Sanchez, Camilo Jaimes, Antonio Perez-Prados, Manuel E. Patarroyo, Manuel A. Patarroyo

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Cervical samples were evaluated for human papillomavirus (HPV) presence using the hybrid capture-2 (HC2) assay and the polymerase chain reaction (PCR) with three different primer sets (GP5+/6+, MY09/11 and pU1M/2R). PCR results were compared to HC2 and results of all assays were compared to cytological and colposcopy findings. Post-test probability was assessed in individual assays and test combinations. HPV-DNA prevalence was 36.5% with HC2 and 55.2% with PCR. MY09/11 detected HPV-DNA in 38% of samples, GP5+/6+ in 19.1% and pU1M/2R in 16.4%. pU1M/2R and HC2 had the highest concordance (75.31%, k= 0.39 in the whole population; 74.1%, k= 0.5 in women with abnormal cytology). pU1M/2R had the best diagnostic performance, including optimal post-test probabilities and cervical abnormality detection (individually or in a panel of tests). Women positive for pU1M/2R may be at higher risk of disease progression; the assay performance when combined with a Pap smear in cervical cancer screening programs should be evaluated.

Original languageEnglish (US)
Pages (from-to)32-38
Number of pages7
JournalJournal of Virological Methods
Issue number1
StatePublished - Oct 2012

All Science Journal Classification (ASJC) codes

  • Virology


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