TY - JOUR
T1 - Th1/Th2 cytokines in patients with systemic lupus erythematosus
T2 - Is tumor necrosis factor α protective?
AU - Gómez, Diana
AU - Correa, Paula A.
AU - Gómez, Luis Miguel
AU - Cadena, José
AU - Molina, José F.
AU - Anaya, Juan Manuel
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2004/6
Y1 - 2004/6
N2 - Objectives To determine the circulating levels of Th1 and Th2 cytokines in patients with systemic lupus erythematosus (SLE) and to elucidate their association with disease activity and autoimmune response. Methods We included 52 patients and 25 healthy controls. Serum levels of tumor necrosis factor (TNF) α, interferon (IFN) γ, interleukin (IL)-12p70, IL-10, and IL-4, as well as anti-DNA, -Ro, -La, -RNP, and -Sm antibodies were determined by enzyme-linked immunosorbent assay. Disease activity was recorded according to the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) and classified as very active (SLEDAI ≥ 13), moderately active (SLEDAI: 3-12), or inactive (SLEDAI ≤ 2). Results The mean age of the patients was 34.2 ± 12.6 years, and the mean duration of disease was 4.9 ± 7.6 years. Twelve patients (23%), 20 patients (34.5%), and 20 patients (34.5%) had highly, moderately, and inactive SLE, respectively. Levels of IFN-γ, TNF-α, and IL-12 were significantly higher in patients than in healthy controls (P < .03), as well as the IL-12/IL-10, IL-12/IL-4, IFN/IL-10, IFN/IL-4, TNF/IL-10, and TNF/IL-4 ratios (P < .01), suggesting a major participation of Th1 over Th2 cytokines. Nevertheless, a direct correlation between Th1 (IFN-γ and TNF-α) and Th2 (IL-4 and IL-10) cytokines was observed in patients (r > .5, P < .01), indicating a mutual Th1-Th2 participation. TNF-α levels and the TNF/IL-10 ratio were higher in patients with inactive disease compared with patients with very active disease and controls (P < .04). IL-12 levels and IL-12/IL-4, as well as IL-12/IL-10, ratios were higher in patients with very active disease than in those with inactive SLE and controls (P < .01). IL-10 levels were associated with anti-DNA, anti-Ro, and anti-La response (P < .01). Conclusion Our results suggest that TNF-α could be a protective factor in SLE patients, whereas IL-12p70 participates in disease activity and IL-10 influences the autoimmune response (autoantibody production).
AB - Objectives To determine the circulating levels of Th1 and Th2 cytokines in patients with systemic lupus erythematosus (SLE) and to elucidate their association with disease activity and autoimmune response. Methods We included 52 patients and 25 healthy controls. Serum levels of tumor necrosis factor (TNF) α, interferon (IFN) γ, interleukin (IL)-12p70, IL-10, and IL-4, as well as anti-DNA, -Ro, -La, -RNP, and -Sm antibodies were determined by enzyme-linked immunosorbent assay. Disease activity was recorded according to the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) and classified as very active (SLEDAI ≥ 13), moderately active (SLEDAI: 3-12), or inactive (SLEDAI ≤ 2). Results The mean age of the patients was 34.2 ± 12.6 years, and the mean duration of disease was 4.9 ± 7.6 years. Twelve patients (23%), 20 patients (34.5%), and 20 patients (34.5%) had highly, moderately, and inactive SLE, respectively. Levels of IFN-γ, TNF-α, and IL-12 were significantly higher in patients than in healthy controls (P < .03), as well as the IL-12/IL-10, IL-12/IL-4, IFN/IL-10, IFN/IL-4, TNF/IL-10, and TNF/IL-4 ratios (P < .01), suggesting a major participation of Th1 over Th2 cytokines. Nevertheless, a direct correlation between Th1 (IFN-γ and TNF-α) and Th2 (IL-4 and IL-10) cytokines was observed in patients (r > .5, P < .01), indicating a mutual Th1-Th2 participation. TNF-α levels and the TNF/IL-10 ratio were higher in patients with inactive disease compared with patients with very active disease and controls (P < .04). IL-12 levels and IL-12/IL-4, as well as IL-12/IL-10, ratios were higher in patients with very active disease than in those with inactive SLE and controls (P < .01). IL-10 levels were associated with anti-DNA, anti-Ro, and anti-La response (P < .01). Conclusion Our results suggest that TNF-α could be a protective factor in SLE patients, whereas IL-12p70 participates in disease activity and IL-10 influences the autoimmune response (autoantibody production).
UR - http://www.scopus.com/inward/record.url?scp=17044461280&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17044461280&partnerID=8YFLogxK
U2 - 10.1016/j.semarthrit.2003.11.002
DO - 10.1016/j.semarthrit.2003.11.002
M3 - Article
C2 - 15190525
AN - SCOPUS:17044461280
SN - 0049-0172
VL - 33
SP - 404
EP - 413
JO - Seminars in Arthritis and Rheumatism
JF - Seminars in Arthritis and Rheumatism
IS - 6
ER -
