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Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

  • Karen Orjuela
  • , Catalina Amador
  • , Manuel A. Patarroyo

Research output: Contribution to Journal › Short survey › peer-review

Abstract

Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection. © 2007 Elsevier Inc. All rights reserved.
Translated title of the contributionGenes sintéticos: una herramienta para identificar genotipos del virus del papiloma humano mediante hibridación y ensayos basados en la reacción en cadena de la polimerasa
Original languageEnglish (US)
Pages (from-to)101-104
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume59
Issue number1
DOIs
StatePublished - Sep 1 2007
Externally publishedYes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

  • Immunology

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