Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Karen Orjuela, Catalina Amador, Manuel A. Patarroyo

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection. © 2007 Elsevier Inc. All rights reserved.
Original languageEnglish (US)
Pages (from-to)101-104
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
DOIs
StatePublished - Sep 1 2007
Externally publishedYes

Fingerprint

Dive into the research topics of 'Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays'. Together they form a unique fingerprint.

Cite this