Staurosporine-induced apoptosis in P388D1 macrophages involves both extrinsic and intrinsic pathways

Yuko Nakamura-López; Rosa Elena Sarmiento-Silva; Julio Moran-Andrade; Beatriz Gómez-García

doi:10.1016/j.cellbi.2009.06.010

Staurosporine-induced apoptosis in P388D1 macrophages involves both extrinsic and intrinsic pathways

Yuko Nakamura-López
, Rosa Elena Sarmiento-Silva
, Julio Moran-Andrade
, Beatriz Gómez-García

Research output: Contribution to Journal › Research Article › peer-review

11 Scopus citations

Abstract

Treatment of P388D1, a macrophage-like cell line, with staurosporine triggered apoptosis through the activation of caspase-9 and caspase-3. Unexpected effects of staurosporine on the induction of apoptosis were the activation of caspase-8, and an increase of the levels of TNF-α. The increased TNF-α levels led to activation of caspase-8 by an autocrine effect via the TNF receptor expressed by the P388D1 macrophages. In contrast, P388D1 macrophages that either had been exposed to UV light or treated with dexamethasone did not undergo apoptosis.

Original language	English (US)
Pages (from-to)	1026-1031
Number of pages	6
Journal	Cell Biology International
Volume	33
Issue number	9
DOIs	https://doi.org/10.1016/j.cellbi.2009.06.010
State	Published - Sep 2009
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Cell Biology

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10.1016/j.cellbi.2009.06.010

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@article{7aa20e7c1a3242ffafd2b746d4d12c95,

title = "Staurosporine-induced apoptosis in P388D1 macrophages involves both extrinsic and intrinsic pathways",

abstract = "Treatment of P388D1, a macrophage-like cell line, with staurosporine triggered apoptosis through the activation of caspase-9 and caspase-3. Unexpected effects of staurosporine on the induction of apoptosis were the activation of caspase-8, and an increase of the levels of TNF-α. The increased TNF-α levels led to activation of caspase-8 by an autocrine effect via the TNF receptor expressed by the P388D1 macrophages. In contrast, P388D1 macrophages that either had been exposed to UV light or treated with dexamethasone did not undergo apoptosis.",

author = "Yuko Nakamura-L{\'o}pez and Sarmiento-Silva, \{Rosa Elena\} and Julio Moran-Andrade and Beatriz G{\'o}mez-Garc{\'i}a",

note = "Funding Information: This work was supported by the Consejo Nacional de Ciencia y Tecnolog{\'i}a (CONACyT; grant \# 43944-M) and by the Direcci{\'o}n General de Asuntos del Personal Academico-Universidad Nacional Autonoma de Mexico (DGAPA-UNAM; grant \# IN2004007). We thank Andi Espinoza-Sanchez and Berenice Hern{\'a}ndez for the technical assistance, Rocio Tirado Mendoza, PhD, for helpful discussions, and Veronica Yakoleff for English revision and for editing of the manuscript. This work is part of the PhD dissertation submitted by Y. Nakamura-Lopez in partial fulfillment of the degree requirements.",

year = "2009",

month = sep,

doi = "10.1016/j.cellbi.2009.06.010",

language = "English (US)",

volume = "33",

pages = "1026--1031",

journal = "Cell Biology International",

issn = "1065-6995",

publisher = "Wiley-Blackwell",

number = "9",

}

TY - JOUR

T1 - Staurosporine-induced apoptosis in P388D1 macrophages involves both extrinsic and intrinsic pathways

AU - Nakamura-López, Yuko

AU - Sarmiento-Silva, Rosa Elena

AU - Moran-Andrade, Julio

AU - Gómez-García, Beatriz

N1 - Funding Information: This work was supported by the Consejo Nacional de Ciencia y Tecnología (CONACyT; grant # 43944-M) and by the Dirección General de Asuntos del Personal Academico-Universidad Nacional Autonoma de Mexico (DGAPA-UNAM; grant # IN2004007). We thank Andi Espinoza-Sanchez and Berenice Hernández for the technical assistance, Rocio Tirado Mendoza, PhD, for helpful discussions, and Veronica Yakoleff for English revision and for editing of the manuscript. This work is part of the PhD dissertation submitted by Y. Nakamura-Lopez in partial fulfillment of the degree requirements.

PY - 2009/9

Y1 - 2009/9

N2 - Treatment of P388D1, a macrophage-like cell line, with staurosporine triggered apoptosis through the activation of caspase-9 and caspase-3. Unexpected effects of staurosporine on the induction of apoptosis were the activation of caspase-8, and an increase of the levels of TNF-α. The increased TNF-α levels led to activation of caspase-8 by an autocrine effect via the TNF receptor expressed by the P388D1 macrophages. In contrast, P388D1 macrophages that either had been exposed to UV light or treated with dexamethasone did not undergo apoptosis.

AB - Treatment of P388D1, a macrophage-like cell line, with staurosporine triggered apoptosis through the activation of caspase-9 and caspase-3. Unexpected effects of staurosporine on the induction of apoptosis were the activation of caspase-8, and an increase of the levels of TNF-α. The increased TNF-α levels led to activation of caspase-8 by an autocrine effect via the TNF receptor expressed by the P388D1 macrophages. In contrast, P388D1 macrophages that either had been exposed to UV light or treated with dexamethasone did not undergo apoptosis.

UR - https://www.scopus.com/pages/publications/68949213602

UR - https://www.scopus.com/pages/publications/68949213602#tab=citedBy

U2 - 10.1016/j.cellbi.2009.06.010

DO - 10.1016/j.cellbi.2009.06.010

M3 - Research Article

C2 - 19524691

AN - SCOPUS:68949213602

SN - 1065-6995

VL - 33

SP - 1026

EP - 1031

JO - Cell Biology International

JF - Cell Biology International

IS - 9

ER -

Staurosporine-induced apoptosis in P388D1 macrophages involves both extrinsic and intrinsic pathways

Abstract

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