Sporozoite and liver stage antigen Plasmodium falciparum peptides bind specifically to human hepatocytes

Adriana Janneth Bermudez Quintero; Alvaro Puentes; Javier García; Ricardo Vera; Ramses Lopez; Jorge Suarez; Hernando Curtidor; Marisol Ocampo Cifuentes; Diana R. Tovar; Martha Forero; Jimena Cortés; Mauricio Urquiza; Manuel Elkin Patarroyo

doi:10.1016/j.vaccine.2003.09.029

Sporozoite and liver stage antigen Plasmodium falciparum peptides bind specifically to human hepatocytes

Adriana Janneth Bermudez Quintero, Alvaro Puentes, Javier García, Ricardo Vera, Ramses Lopez, Jorge Suarez, Hernando Curtidor, Marisol Ocampo Cifuentes, Diana R. Tovar, Martha Forero, Jimena Cortés, Mauricio Urquiza, Manuel Elkin Patarroyo

School of Medicine and Health Sciences

Research output: Contribution to journal › Research Article › peer-review

14 Scopus citations

Abstract

Sporozoite and Liver Stage Antigen (SALSA) sequence synthetic peptides were used in HepG2 cell binding assays to identify regions involved in parasite invasion. SALSA 20608 ( 21IWASEKKDEKEASEQGEESHY40) and 20611 ( 64KKDDGTDKVQEKVLEKSPKY83) peptides were determined as having high binding activity in HepG2 cell assays, some of them were located in immunogenic regions. Immune-fluorescence antibody test with 24276 (20608 peptide analogue, CGIWSSMKMDEKMAAMQGEESHCG) showed sporozoite and merozoite reactivity. This data suggests SALSA high activity binding peptides' (HABPs) possible role in hepatic cell invasion and merozoite invasion of erythrocytes

Original language	English (US)
Pages (from-to)	1150-1156
Number of pages	7
Journal	Vaccine
Volume	22
Issue number	9-10
DOIs	https://doi.org/10.1016/j.vaccine.2003.09.029 https://doi.org/10.1016/j.vaccine.2003.09.029
State	Published - Mar 12 2004

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Bermudez Quintero, A. J., Puentes, A., García, J., Vera, R., Lopez, R., Suarez, J., Curtidor, H., Ocampo Cifuentes, M., Tovar, D. R., Forero, M., Cortés, J., Urquiza, M., & Patarroyo, M. E. (2004). Sporozoite and liver stage antigen Plasmodium falciparum peptides bind specifically to human hepatocytes. Vaccine, 22(9-10), 1150-1156. https://doi.org/10.1016/j.vaccine.2003.09.029, https://doi.org/10.1016/j.vaccine.2003.09.029

@article{3be16e9782b342d894c3bcc628ab4286,

title = "Sporozoite and liver stage antigen Plasmodium falciparum peptides bind specifically to human hepatocytes",

abstract = "Sporozoite and Liver Stage Antigen (SALSA) sequence synthetic peptides were used in HepG2 cell binding assays to identify regions involved in parasite invasion. SALSA 20608 ( 21IWASEKKDEKEASEQGEESHY40) and 20611 ( 64KKDDGTDKVQEKVLEKSPKY83) peptides were determined as having high binding activity in HepG2 cell assays, some of them were located in immunogenic regions. Immune-fluorescence antibody test with 24276 (20608 peptide analogue, CGIWSSMKMDEKMAAMQGEESHCG) showed sporozoite and merozoite reactivity. This data suggests SALSA high activity binding peptides' (HABPs) possible role in hepatic cell invasion and merozoite invasion of erythrocytes",

author = "{Bermudez Quintero}, {Adriana Janneth} and Alvaro Puentes and Javier Garc{\'i}a and Ricardo Vera and Ramses Lopez and Jorge Suarez and Hernando Curtidor and {Ocampo Cifuentes}, Marisol and Tovar, {Diana R.} and Martha Forero and Jimena Cort{\'e}s and Mauricio Urquiza and Patarroyo, {Manuel Elkin}",

year = "2004",

month = mar,

day = "12",

doi = "10.1016/j.vaccine.2003.09.029",

language = "English (US)",

volume = "22",

pages = "1150--1156",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier BV",

number = "9-10",

}

Bermudez Quintero, AJ, Puentes, A, García, J, Vera, R, Lopez, R, Suarez, J, Curtidor, H, Ocampo Cifuentes, M, Tovar, DR, Forero, M, Cortés, J, Urquiza, M & Patarroyo, ME 2004, 'Sporozoite and liver stage antigen Plasmodium falciparum peptides bind specifically to human hepatocytes', Vaccine, vol. 22, no. 9-10, pp. 1150-1156. https://doi.org/10.1016/j.vaccine.2003.09.029, https://doi.org/10.1016/j.vaccine.2003.09.029

TY - JOUR

T1 - Sporozoite and liver stage antigen Plasmodium falciparum peptides bind specifically to human hepatocytes

AU - Bermudez Quintero, Adriana Janneth

AU - Puentes, Alvaro

AU - García, Javier

AU - Vera, Ricardo

AU - Lopez, Ramses

AU - Suarez, Jorge

AU - Curtidor, Hernando

AU - Ocampo Cifuentes, Marisol

AU - Tovar, Diana R.

AU - Forero, Martha

AU - Cortés, Jimena

AU - Urquiza, Mauricio

AU - Patarroyo, Manuel Elkin

PY - 2004/3/12

Y1 - 2004/3/12

N2 - Sporozoite and Liver Stage Antigen (SALSA) sequence synthetic peptides were used in HepG2 cell binding assays to identify regions involved in parasite invasion. SALSA 20608 ( 21IWASEKKDEKEASEQGEESHY40) and 20611 ( 64KKDDGTDKVQEKVLEKSPKY83) peptides were determined as having high binding activity in HepG2 cell assays, some of them were located in immunogenic regions. Immune-fluorescence antibody test with 24276 (20608 peptide analogue, CGIWSSMKMDEKMAAMQGEESHCG) showed sporozoite and merozoite reactivity. This data suggests SALSA high activity binding peptides' (HABPs) possible role in hepatic cell invasion and merozoite invasion of erythrocytes

AB - Sporozoite and Liver Stage Antigen (SALSA) sequence synthetic peptides were used in HepG2 cell binding assays to identify regions involved in parasite invasion. SALSA 20608 ( 21IWASEKKDEKEASEQGEESHY40) and 20611 ( 64KKDDGTDKVQEKVLEKSPKY83) peptides were determined as having high binding activity in HepG2 cell assays, some of them were located in immunogenic regions. Immune-fluorescence antibody test with 24276 (20608 peptide analogue, CGIWSSMKMDEKMAAMQGEESHCG) showed sporozoite and merozoite reactivity. This data suggests SALSA high activity binding peptides' (HABPs) possible role in hepatic cell invasion and merozoite invasion of erythrocytes

UR - http://www.sciencedirect.com/science/article/pii/S0264410X03006972?via%3Dihub

U2 - 10.1016/j.vaccine.2003.09.029

DO - 10.1016/j.vaccine.2003.09.029

M3 - Research Article

C2 - 15003642

SN - 0264-410X

VL - 22

SP - 1150

EP - 1156

JO - Vaccine

JF - Vaccine

IS - 9-10

ER -

Sporozoite and liver stage antigen Plasmodium falciparum peptides bind specifically to human hepatocytes

Abstract

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