Serum activity of matrix metalloproteinase-2 and -9 is increased in chronic post-stroke individuals: a cross-sectional exploratory study

Luisa Fernanda García-Salazar, Jean Alex Matos Ribeiro, Jonathan Emanuel Cunha, Stela Marcia Mattiello, Thiago Luiz Russo

Research output: Contribution to journalResearch Articlepeer-review

4 Scopus citations


Background: High concentrations of matrix metalloproteinase-2 (MMP-2) and −9 (MMP-9) are associated with inflammation early after stroke. In chronic stages, the elevation of some cytokines is related to the presence of co-morbid conditions in these individuals. In addition to this, some characteristics such as sensorimotor impairment, atrophy, and sedentary lifestyle predispose the system to an inflammatory response. Objective: To quantify MMP-2 and −9 serum activity in chronic post-stroke individuals and correlate it with variables of physical activity level, body composition, functional and walking capacity, and with inflammatory biomarkers. Additionally, gelatinase activity was characterized according to motor impairment. Methods: Fourteen patients with stroke onset >6 months and seven healthy individuals were enrolled in this study. The clinical assessment included: body composition, measure by bioelectrical impedance analyzer; Fugl-Meyer Motor Assessment Scale; six-minute and ten-meter walk tests, and physical activity level assessed by the StepWatch® Activity Monitor. Blood samples were collected from antecubital vein and serum MMP-2 and −9 activity was analyzed using gelatin Zymography, and the TNFα, IL-6, IL-1β, IL-10 biomarkers using ELISA kits. Results: Chronic post-stroke individuals presented an increased activity of MMP-2 and −9 compared to healthy individuals. Positive correlations with time and steps in low cadence and negative ones with medium cadence and peak activity index were observed. According to the motor impairment, the MMP-2 activity was increased in the mild-moderate group compared to the control group. Conclusion: Increased gelatinases in chronic post-stroke individuals could describe an inflammation process related to the limited capacity of walking in high intensities. 

Translated title of the contributionLa actividad sérica de las metaloproteinasas de matriz-2 y -9 está aumentada en individuos con post-accidente cerebrovascular crónico: un estudio exploratorio transversal
Original languageEnglish (US)
Pages (from-to)605-615
Number of pages10
JournalTopics in Stroke Rehabilitation
Issue number8
StateE-pub ahead of print - Aug 2 2021

All Science Journal Classification (ASJC) codes

  • Rehabilitation
  • Community and Home Care
  • Clinical Neurology


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