RNA-seq in kinetoplastids: A powerful tool for the understanding of the biology and host-pathogen interactions

Luz Helena Patino, Juan David Ramírez

Research output: Contribution to journalLiterature review

7 Citations (Scopus)

Abstract

© 2017 Elsevier B.V.The kinetoplastids include a large number of parasites responsible for serious diseases in humans and animals (Leishmania and Trypanosoma brucei) considered endemic in several regions of the world. These parasites are characterized by digenetic life cycles that undergo morphological and genetic changes that allow them to adapt to different microenvironments on their vertebrates and invertebrates hosts. Recent advances in ´omics´ technology, specifically transcriptomics have allowed to reveal aspects associated with such molecular changes. So far, different techniques have been used to evaluate the gene expression profile during the various stages of the life cycle of these parasites and during the host-parasite interactions. However, some of them have serious drawbacks that limit the precise study and full understanding of their transcriptomes. Therefore, recently has been implemented the latest technology (RNA-seq), which overcomes the drawbacks of traditional methods. In this review, studies that so far have used RNA-seq are presented and allowed to expand our knowledge regarding the biology of these parasites and their interactions with their hosts.
Original languageEnglish (US)
Pages (from-to)273-282
Number of pages10
JournalInfection, Genetics and Evolution
DOIs
StatePublished - Apr 1 2017

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host-pathogen interaction
Host-Pathogen Interactions
host-pathogen relationships
RNA
parasite
Parasites
parasites
Biological Sciences
Life Cycle Stages
Transcriptome
life cycle
Technology
host-parasite interaction
diseases and disorders (animals and humans)
Host-Parasite Interactions
Trypanosoma brucei
Trypanosoma brucei brucei
Leishmania
host-parasite relationships
Invertebrates

Cite this

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title = "RNA-seq in kinetoplastids: A powerful tool for the understanding of the biology and host-pathogen interactions",
abstract = "{\circledC} 2017 Elsevier B.V.The kinetoplastids include a large number of parasites responsible for serious diseases in humans and animals (Leishmania and Trypanosoma brucei) considered endemic in several regions of the world. These parasites are characterized by digenetic life cycles that undergo morphological and genetic changes that allow them to adapt to different microenvironments on their vertebrates and invertebrates hosts. Recent advances in ´omics´ technology, specifically transcriptomics have allowed to reveal aspects associated with such molecular changes. So far, different techniques have been used to evaluate the gene expression profile during the various stages of the life cycle of these parasites and during the host-parasite interactions. However, some of them have serious drawbacks that limit the precise study and full understanding of their transcriptomes. Therefore, recently has been implemented the latest technology (RNA-seq), which overcomes the drawbacks of traditional methods. In this review, studies that so far have used RNA-seq are presented and allowed to expand our knowledge regarding the biology of these parasites and their interactions with their hosts.",
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RNA-seq in kinetoplastids: A powerful tool for the understanding of the biology and host-pathogen interactions. / Patino, Luz Helena; Ramírez, Juan David.

In: Infection, Genetics and Evolution, 01.04.2017, p. 273-282.

Research output: Contribution to journalLiterature review

TY - JOUR

T1 - RNA-seq in kinetoplastids: A powerful tool for the understanding of the biology and host-pathogen interactions

AU - Patino, Luz Helena

AU - Ramírez, Juan David

PY - 2017/4/1

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N2 - © 2017 Elsevier B.V.The kinetoplastids include a large number of parasites responsible for serious diseases in humans and animals (Leishmania and Trypanosoma brucei) considered endemic in several regions of the world. These parasites are characterized by digenetic life cycles that undergo morphological and genetic changes that allow them to adapt to different microenvironments on their vertebrates and invertebrates hosts. Recent advances in ´omics´ technology, specifically transcriptomics have allowed to reveal aspects associated with such molecular changes. So far, different techniques have been used to evaluate the gene expression profile during the various stages of the life cycle of these parasites and during the host-parasite interactions. However, some of them have serious drawbacks that limit the precise study and full understanding of their transcriptomes. Therefore, recently has been implemented the latest technology (RNA-seq), which overcomes the drawbacks of traditional methods. In this review, studies that so far have used RNA-seq are presented and allowed to expand our knowledge regarding the biology of these parasites and their interactions with their hosts.

AB - © 2017 Elsevier B.V.The kinetoplastids include a large number of parasites responsible for serious diseases in humans and animals (Leishmania and Trypanosoma brucei) considered endemic in several regions of the world. These parasites are characterized by digenetic life cycles that undergo morphological and genetic changes that allow them to adapt to different microenvironments on their vertebrates and invertebrates hosts. Recent advances in ´omics´ technology, specifically transcriptomics have allowed to reveal aspects associated with such molecular changes. So far, different techniques have been used to evaluate the gene expression profile during the various stages of the life cycle of these parasites and during the host-parasite interactions. However, some of them have serious drawbacks that limit the precise study and full understanding of their transcriptomes. Therefore, recently has been implemented the latest technology (RNA-seq), which overcomes the drawbacks of traditional methods. In this review, studies that so far have used RNA-seq are presented and allowed to expand our knowledge regarding the biology of these parasites and their interactions with their hosts.

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JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

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