Plasmodium vivax in vitro continuous culture: the spoke in the wheel

Maritza Bermúdez, Darwin Andrés Moreno-Pérez, Gabriela Arévalo-Pinzón, Hernando Curtidor, Manuel Alfonso Patarroyo

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

Understanding the life cycle of Plasmodium vivax is fundamental for developing strategies aimed at controlling and eliminating this parasitic species. Although advances in omic sciences and high-throughput techniques in recent years have enabled the identification and characterization of proteins which might be participating in P. vivax invasion of target cells, exclusive parasite tropism for invading reticulocytes has become the main obstacle in maintaining a continuous culture for this species. Such advance that would help in defining each parasite protein's function in the complex process of P. vivax invasion, in addition to evaluating new therapeutic agents, is still a dream. Advances related to maintenance, culture medium supplements and the use of different sources of reticulocytes and parasites (strains and isolates) have been made regarding the development of an in vitro culture for P. vivax; however, only some cultures having few replication cycles have been obtained to date, meaning that this parasite's maintenance goes beyond the technical components involved. Although it is still not yet clear which molecular mechanisms P. vivax prefers for invading young CD71+ reticulocytes [early maturation stages (I-II-III)], changes related to membrane proteins remodelling of such cells could form part of the explanation. The most relevant aspects regarding P. vivax in vitro culture and host cell characteristics have been analysed in this review to explain possible reasons why the species' continuous in vitro culture is so difficult to standardize. Some alternatives for P. vivax in vitro culture have also been described.

Original languageEnglish (US)
Pages (from-to)301
JournalMalaria Journal
Volume17
Issue number1
DOIs
StatePublished - Aug 20 2018

Cite this

Bermúdez, Maritza ; Moreno-Pérez, Darwin Andrés ; Arévalo-Pinzón, Gabriela ; Curtidor, Hernando ; Patarroyo, Manuel Alfonso. / Plasmodium vivax in vitro continuous culture : the spoke in the wheel. In: Malaria Journal. 2018 ; Vol. 17, No. 1. pp. 301.
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abstract = "Understanding the life cycle of Plasmodium vivax is fundamental for developing strategies aimed at controlling and eliminating this parasitic species. Although advances in omic sciences and high-throughput techniques in recent years have enabled the identification and characterization of proteins which might be participating in P. vivax invasion of target cells, exclusive parasite tropism for invading reticulocytes has become the main obstacle in maintaining a continuous culture for this species. Such advance that would help in defining each parasite protein's function in the complex process of P. vivax invasion, in addition to evaluating new therapeutic agents, is still a dream. Advances related to maintenance, culture medium supplements and the use of different sources of reticulocytes and parasites (strains and isolates) have been made regarding the development of an in vitro culture for P. vivax; however, only some cultures having few replication cycles have been obtained to date, meaning that this parasite's maintenance goes beyond the technical components involved. Although it is still not yet clear which molecular mechanisms P. vivax prefers for invading young CD71+ reticulocytes [early maturation stages (I-II-III)], changes related to membrane proteins remodelling of such cells could form part of the explanation. The most relevant aspects regarding P. vivax in vitro culture and host cell characteristics have been analysed in this review to explain possible reasons why the species' continuous in vitro culture is so difficult to standardize. Some alternatives for P. vivax in vitro culture have also been described.",
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Plasmodium vivax in vitro continuous culture : the spoke in the wheel. / Bermúdez, Maritza; Moreno-Pérez, Darwin Andrés; Arévalo-Pinzón, Gabriela; Curtidor, Hernando; Patarroyo, Manuel Alfonso.

In: Malaria Journal, Vol. 17, No. 1, 20.08.2018, p. 301.

Research output: Contribution to journalReview article

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T2 - the spoke in the wheel

AU - Bermúdez, Maritza

AU - Moreno-Pérez, Darwin Andrés

AU - Arévalo-Pinzón, Gabriela

AU - Curtidor, Hernando

AU - Patarroyo, Manuel Alfonso

PY - 2018/8/20

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