TY - JOUR
T1 - Phylogenomic evidence of reinfection and persistence of sars-cov-2
T2 - First report from Colombia
AU - Ramírez, Juan David
AU - Muñoz, Marina
AU - Ballesteros, Nathalia
AU - Patiño, Luz H.
AU - Castañeda, Sergio
AU - Rincón, Carlos A.
AU - Mendez, Claudia
AU - Oliveros, Carolina
AU - Perez, Julie
AU - Márquez, Elizabeth K.
AU - Ortiz, Frank de los Santos
AU - Correa-Cárdenas, Camilo A.
AU - Duque, Maria Clara
AU - Paniz-Mondolfi, Alberto
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/3
Y1 - 2021/3
N2 - The continuing evolution of SARS-CoV-2 and the emergence of novel variants have raised concerns about possible reinfection events and potential changes in the coronavirus disease 2019 (COVID-19) transmission dynamics. Utilizing Oxford Nanopore technologies, we sequenced paired samples of three patients with positive RT-PCR results in a 1–2-month window period, and subsequent phylogenetics and genetic polymorphism analysis of these genomes was performed. Herein, we report, for the first time, genomic evidence of one case of reinfection in Colombia, exhibiting different SARS-CoV-2 lineage classifications between samples (B.1 and B.1.1.269). Furthermore, we report two cases of possible viral persistence, highlighting the importance of deepening our understanding on the evolutionary intra-host traits of this virus throughout different timeframes of disease progression. These results emphasize the relevance of genomic surveillance as a tool for understanding SARS-CoV-2 infection dynamics, and how this may translate effectively to future control and mitigations efforts, such as the national vaccination program.
AB - The continuing evolution of SARS-CoV-2 and the emergence of novel variants have raised concerns about possible reinfection events and potential changes in the coronavirus disease 2019 (COVID-19) transmission dynamics. Utilizing Oxford Nanopore technologies, we sequenced paired samples of three patients with positive RT-PCR results in a 1–2-month window period, and subsequent phylogenetics and genetic polymorphism analysis of these genomes was performed. Herein, we report, for the first time, genomic evidence of one case of reinfection in Colombia, exhibiting different SARS-CoV-2 lineage classifications between samples (B.1 and B.1.1.269). Furthermore, we report two cases of possible viral persistence, highlighting the importance of deepening our understanding on the evolutionary intra-host traits of this virus throughout different timeframes of disease progression. These results emphasize the relevance of genomic surveillance as a tool for understanding SARS-CoV-2 infection dynamics, and how this may translate effectively to future control and mitigations efforts, such as the national vaccination program.
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U2 - 10.3390/vaccines9030282
DO - 10.3390/vaccines9030282
M3 - Article
C2 - 33808687
AN - SCOPUS:85103484211
SN - 2076-393X
VL - 9
JO - Vaccines
JF - Vaccines
IS - 3
M1 - 282
ER -
