TY - JOUR
T1 - Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry
T2 - Potential vaccine candidates?
AU - Patarroyo, Manuel A.
AU - Curtidor, Hernando
AU - Plaza, David F.
AU - Ocampo, Marisol
AU - Reyes, Claudia
AU - Saboya, Obeimar
AU - Barrera, Gloria
AU - Patarroyo, Manuel E.
N1 - Funding Information:
This study was started by Dr. Fabio Castillo and John Valbuena when working at FIDIC some years ago, their collaboration is whole-heartedly acknowledged. We would like to thank the San Juan de Dios and Santa Clara hospital for providing us with clinical samples. Special thanks go to the Secretary of Health's Mycobacteria Laboratory and Dr. Gloria Amparo Mejía for the mycobacterial strains being donated for this study. This research has been supported by COLCIENCIAS; contract RC041-2007. Jason Garry's and Nora Martinez’ help in translating this manuscript is appreciated.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/8/12
Y1 - 2008/8/12
N2 - This study reports the Rv1490 gene presence and transcription in members of the Mycobacterium tuberculosis complex, and characterises the encoded Rv1490 putative membrane protein in M. tuberculosis H37Rv. Rv1490 derived peptides were synthesised and their A549 and U937 cell binding ability was tested, finding five high activity binding peptides (HABPs) for A549 and five for U937. Only two HABPs (11060 and 11073) were shared by both cell lines, both of which affected M. tuberculosis' invading ability to target cells, thus indicating an important role for these sequences in M. tuberculosis entry to A549 alveolar epithelial cells and supporting their inclusion in further studies on the development of a subunit-based multi-epitopic, chemically synthesised anti-tuberculosis vaccine.
AB - This study reports the Rv1490 gene presence and transcription in members of the Mycobacterium tuberculosis complex, and characterises the encoded Rv1490 putative membrane protein in M. tuberculosis H37Rv. Rv1490 derived peptides were synthesised and their A549 and U937 cell binding ability was tested, finding five high activity binding peptides (HABPs) for A549 and five for U937. Only two HABPs (11060 and 11073) were shared by both cell lines, both of which affected M. tuberculosis' invading ability to target cells, thus indicating an important role for these sequences in M. tuberculosis entry to A549 alveolar epithelial cells and supporting their inclusion in further studies on the development of a subunit-based multi-epitopic, chemically synthesised anti-tuberculosis vaccine.
UR - http://www.scopus.com/inward/record.url?scp=47649132993&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=47649132993&partnerID=8YFLogxK
U2 - 10.1016/j.vaccine.2008.05.092
DO - 10.1016/j.vaccine.2008.05.092
M3 - Article
C2 - 18585422
AN - SCOPUS:47649132993
SN - 0264-410X
VL - 26
SP - 4387
EP - 4395
JO - Vaccine
JF - Vaccine
IS - 34
ER -
