Palindromic Peptide LfcinB (21-25)Pal Exhibited Antifungal Activity against Multidrug-Resistant Candida

Yerly Vargas-Casanova, Jean Carlos Villamil Poveda, Zuly Jenny Rivera-Monroy, Andrés Ceballos Garzón, Ricardo Fierro-Medina, Patrice Le Pape, Javier Eduardo García-Castañeda, Claudia Marcela Parra Giraldo

Research output: Contribution to journalResearch Articlepeer-review

14 Scopus citations

Abstract

Palindromic peptide LfcinB (21–25)Pal: RWQWRWQWR was synthesized by Solid Phase Peptide Synthesis (SPPS-Fmoc/tBu), purified by Reverse Phase Solid Phase Extraction (RP-SPE) and characterized by Reverse Phase High Performance Liquid Chromatography (RP-HPLC) and Matrix-Assisted Laser Desorption/Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS). The antifungal activity of LfcinB (21–25)Pal against both ATCC strains and clinical isolates of C. albicans, C. glabrata, C. krusei, C. auris and C. tropicalis was evaluated. The palindromic peptide exhibited fungistatic and fungicidal activity against all yeast evaluated. The antifungal activity was dependent on peptide concentration in all cases. Additionally, LfcinB (21–25)Pal (25–50 μg/mL) combined with fluconazole exhibited a synergistic antifungal effect against C. tropicalis 883 and C. krusei 6258 (resistant to fluconazole). This study showed that the palindromic peptide derived from Lactoferricin B (LfcinB) exhibited significant antifungal activity against Candida spp, suggesting that this peptide could have a therapeutic application solely or in combination with fluconazole.

Original languageEnglish (US)
Pages (from-to)7236-7242
Number of pages7
JournalChemistrySelect
Volume5
Issue number24
DOIs
StatePublished - Jun 30 2020
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Chemistry

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