TY - JOUR
T1 - P. falciparum
T2 - Merezoite Surface Protein-8 peptides bind specifically to human erythrocytes.
AU - Vanegas Murcia, Magnolia
AU - Puentes, Alvaro
AU - García, Javier
AU - Ocampo Cifuentes, Marisol
AU - Rodriguez, Luis Eduardo
AU - Vera, Ricardo
AU - Curtidor Castellanos, Hernando
AU - Lopez, Ramses
AU - Suarez, Jorge
AU - Guzmán, Fanny
AU - Tovar, Diana R.
AU - Patarroyo, Manuel Elkin
PY - 2003
Y1 - 2003
N2 - This work determined Plasmodium falciparum merozoite surface protein-8 (MSP-8) regions specifically binding to membrane surface receptors on human erythrocytes. Five high activity binding peptides (HABPs), whose binding to erythrocytes became saturable and sensitive on being treated with neuraminidase and chymotrypsin were identified from the MSP-8 protein. Those amino acids directly involved in interaction with erythrocytes were also determined for each one of the HABPs. Some of them specifically recognized 28, 46, and 73 kDa erythrocyte membrane proteins. Some HABPs inhibited in vitro P. falciparum merozoite invasion of erythrocytes by up to 98%, suggesting the MSP-8 protein’s possible role in the invasion process.
AB - This work determined Plasmodium falciparum merozoite surface protein-8 (MSP-8) regions specifically binding to membrane surface receptors on human erythrocytes. Five high activity binding peptides (HABPs), whose binding to erythrocytes became saturable and sensitive on being treated with neuraminidase and chymotrypsin were identified from the MSP-8 protein. Those amino acids directly involved in interaction with erythrocytes were also determined for each one of the HABPs. Some of them specifically recognized 28, 46, and 73 kDa erythrocyte membrane proteins. Some HABPs inhibited in vitro P. falciparum merozoite invasion of erythrocytes by up to 98%, suggesting the MSP-8 protein’s possible role in the invasion process.
M3 - Article
SN - 0196-9781
VL - 24
SP - 1015
EP - 1023
JO - Peptides
JF - Peptides
IS - 7
ER -