Long-term storage and safe retrieval of human papillomavirus DNA using FTA elute cards

Heidi Barth, Adrien Morel, Christiane Mougin, Gerlinde Averous, Michèle Legrain, Muriel Fender, Simone Risch, Samira Fafi-Kremer, Michel Velten, Pierre Oudet, Jean Jacques Baldauf, Françoise Stoll-Keller

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Biobanking or collection and storage of specimens for future research purposes have become an essential tool in many fields of biomedical research and aims to provide a better understanding of disease mechanisms as well as the identification of disease-specific biomarkers that can navigate in complex diseases. In this study, we assessed the use of Flinders Technology Associates (FTA) cards as a long-term storage device for cervical specimens with suspected human papillomavirus (HPV) infections. HPV detection and genotyping results in liquid-based transport media were compared to HPV results from FTA cards. The overall agreement for the presence of any HPV infection between liquid-based medium and FTA cards stored for 1 year at ambient temperature was 100%. Reproducibility analysis of HPV detection and genotyping from FTA cards demonstrated that FTA cards are a reliable medium to store and preserve viral nucleic acids. Biobanking of cervical cells on FTA cards may provide a key resource for epidemiological and retrospective HPV studies.

Original languageEnglish (US)
Pages (from-to)60-65
Number of pages6
JournalJournal of Virological Methods
Volume229
DOIs
StatePublished - Mar 1 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology

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