Long-term storage and safe retrieval of human papillomavirus DNA using FTA elute cards

Heidi Barth, Adrien Morel, Christiane Mougin, Gerlinde Averous, Michèle Legrain, Muriel Fender, Simone Risch, Samira Fafi-Kremer, Michel Velten, Pierre Oudet, Jean Jacques Baldauf, Françoise Stoll-Keller

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Biobanking or collection and storage of specimens for future research purposes have become an essential tool in many fields of biomedical research and aims to provide a better understanding of disease mechanisms as well as the identification of disease-specific biomarkers that can navigate in complex diseases. In this study, we assessed the use of Flinders Technology Associates (FTA) cards as a long-term storage device for cervical specimens with suspected human papillomavirus (HPV) infections. HPV detection and genotyping results in liquid-based transport media were compared to HPV results from FTA cards. The overall agreement for the presence of any HPV infection between liquid-based medium and FTA cards stored for 1 year at ambient temperature was 100%. Reproducibility analysis of HPV detection and genotyping from FTA cards demonstrated that FTA cards are a reliable medium to store and preserve viral nucleic acids. Biobanking of cervical cells on FTA cards may provide a key resource for epidemiological and retrospective HPV studies.

Original languageEnglish (US)
Pages (from-to)60-65
Number of pages6
JournalJournal of Virological Methods
StatePublished - Mar 1 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology


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