Long-term feeding of the cis-9,trans-11 isomer of conjugated linoleic acid reinforces the specific immune response in rats

Carolina Ramírez-Santana, Cristina Castellote, Margarida Castell, Montserrat Rivero, Maria Rodríguez-Palmero, Àngels Franch, Francisco J. Pérez-Cano

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24 Scopus citations


Several effects on the immune system have been ascribed to the c/s9,trans11 conjugated linoleic acid (CLA) isomer. We studied whether feeding a diet enriched with an 80:20 CLA isomer mix of c/s9,frans11 and trans10,c/S12 CLA from gestation to adulthood affects the capacity of adult rats to achieve a specific immune response. Pregnant Wistar rats were fed a 1% CLA diet or a control diet beginning on d 7 of gestation. Weaned pups received the same diet as dams until they were 15 wk old. Rats from both groups were immunized with ovalbumin (OVA) when they were 9 wk old. Dietary CLA enhanced splenocyte OVA-specific proliferation by ∼50% (P < 0.05) and decreased the mitogen-induced proliferative responses of these cells by ∼10-20% (P< 0.05). The diminished splenocyte proliferative response was accompanied by a lower interleukin-2 secretion (P < 0.05). Long-term CLA supplementation did not increase serum, spleen, or mesenteric lymph node production of OVA-specific antibodies (Ab) or the number of spleen anti-OVA Ab-secreting cells. Interestingly, dietary CLA increased intestinal anti-OVA IgA production by ∼75% (P < 0.05). In conclusion, a 1% CLA diet administered from gestation to adulthood enhanced specific systemic cell-mediated immunity as well as the mucosal IgA immune response, whereas it downregulated the polyclonal activation of the immune system. These data support the long-term effects of dietary c/S9,trans11 CLA isomer on the immune system.

Original languageEnglish (US)
Pages (from-to)76-81
Number of pages6
JournalJournal of Nutrition
Issue number1
StatePublished - Jan 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics


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