Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys: A potential vaccine candidate

Jose Rojas-Caraballo; Alvaro Mongui; Manuel A. Giraldo; Gabriela Delgado; Diana Granados; Diana Millan-Cortes; Paola Martinez; Raul Rodriguez; Manuel A. Patarroyo

doi:10.1016/j.vaccine.2009.02.083

Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys: A potential vaccine candidate

Jose Rojas-Caraballo, Alvaro Mongui, Manuel A. Giraldo, Gabriela Delgado, Diana Granados, Diana Millan-Cortes, Paola Martinez, Raul Rodriguez, Manuel A. Patarroyo

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Abstract

Rhoptry proteins have been extensively shown to be important in invasion and parasitophorous vacuole (PV) formation. This work evaluates the immunogenicity and protective efficacy of Plasmodium vivax RAP2 in the non-human Aotus primate model, when expressed as a recombinant molecule in E. coli and formulated in Freund and Alum hydroxide adjuvants. Our results show that rPvRAP2 is immunogenic in both formulations, finding a trend of higher cytokine levels in immunized monkeys, specially in IL-4 levels (using Freund's adjuvant) and IL-5 (using Alum hydroxide). RAP2 is suggested as a P. vivax-vaccine candidate since immunized monkeys exhibited lower parasitemias than control groups after being experimentally challenged with the P. vivax VCG-I strain.

Original language	English (US)
Pages (from-to)	2870-2876
Number of pages	7
Journal	Vaccine
Volume	27
Issue number	21
DOIs	https://doi.org/10.1016/j.vaccine.2009.02.083
State	Published - May 11 2009

All Science Journal Classification (ASJC) codes

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2009.02.083

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Rojas-Caraballo, J., Mongui, A., Giraldo, M. A., Delgado, G., Granados, D., Millan-Cortes, D., Martinez, P., Rodriguez, R., & Patarroyo, M. A. (2009). Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys: A potential vaccine candidate. Vaccine, 27(21), 2870-2876. https://doi.org/10.1016/j.vaccine.2009.02.083

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title = "Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys: A potential vaccine candidate",

abstract = "Rhoptry proteins have been extensively shown to be important in invasion and parasitophorous vacuole (PV) formation. This work evaluates the immunogenicity and protective efficacy of Plasmodium vivax RAP2 in the non-human Aotus primate model, when expressed as a recombinant molecule in E. coli and formulated in Freund and Alum hydroxide adjuvants. Our results show that rPvRAP2 is immunogenic in both formulations, finding a trend of higher cytokine levels in immunized monkeys, specially in IL-4 levels (using Freund's adjuvant) and IL-5 (using Alum hydroxide). RAP2 is suggested as a P. vivax-vaccine candidate since immunized monkeys exhibited lower parasitemias than control groups after being experimentally challenged with the P. vivax VCG-I strain.",

author = "Jose Rojas-Caraballo and Alvaro Mongui and Giraldo, {Manuel A.} and Gabriela Delgado and Diana Granados and Diana Millan-Cortes and Paola Martinez and Raul Rodriguez and Patarroyo, {Manuel A.}",

note = "Funding Information: We thank Luis Carlos Esparragoza and Gabriela Ar{\'e}valo for their technical support. We would also like to thank Nora Martinez for reviewing this manuscript. Special gratitude goes to Professor Manuel Elkin Patarroyo for his invaluable comments and suggestions. This research was supported by the “Instituto Colombiano para el Desarrollo de la Ciencia {\textquoteleft}Francisco Jose de Caldas{\textquoteright} (COLCIENCIAS)” contract RC-2008. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

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doi = "10.1016/j.vaccine.2009.02.083",

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Rojas-Caraballo, J, Mongui, A, Giraldo, MA, Delgado, G, Granados, D, Millan-Cortes, D, Martinez, P, Rodriguez, R & Patarroyo, MA 2009, 'Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys: A potential vaccine candidate', Vaccine, vol. 27, no. 21, pp. 2870-2876. https://doi.org/10.1016/j.vaccine.2009.02.083

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T1 - Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys

T2 - A potential vaccine candidate

AU - Rojas-Caraballo, Jose

AU - Mongui, Alvaro

AU - Giraldo, Manuel A.

AU - Delgado, Gabriela

AU - Granados, Diana

AU - Millan-Cortes, Diana

AU - Martinez, Paola

AU - Rodriguez, Raul

AU - Patarroyo, Manuel A.

N1 - Funding Information: We thank Luis Carlos Esparragoza and Gabriela Arévalo for their technical support. We would also like to thank Nora Martinez for reviewing this manuscript. Special gratitude goes to Professor Manuel Elkin Patarroyo for his invaluable comments and suggestions. This research was supported by the “Instituto Colombiano para el Desarrollo de la Ciencia ‘Francisco Jose de Caldas’ (COLCIENCIAS)” contract RC-2008. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2009/5/11

Y1 - 2009/5/11

N2 - Rhoptry proteins have been extensively shown to be important in invasion and parasitophorous vacuole (PV) formation. This work evaluates the immunogenicity and protective efficacy of Plasmodium vivax RAP2 in the non-human Aotus primate model, when expressed as a recombinant molecule in E. coli and formulated in Freund and Alum hydroxide adjuvants. Our results show that rPvRAP2 is immunogenic in both formulations, finding a trend of higher cytokine levels in immunized monkeys, specially in IL-4 levels (using Freund's adjuvant) and IL-5 (using Alum hydroxide). RAP2 is suggested as a P. vivax-vaccine candidate since immunized monkeys exhibited lower parasitemias than control groups after being experimentally challenged with the P. vivax VCG-I strain.

AB - Rhoptry proteins have been extensively shown to be important in invasion and parasitophorous vacuole (PV) formation. This work evaluates the immunogenicity and protective efficacy of Plasmodium vivax RAP2 in the non-human Aotus primate model, when expressed as a recombinant molecule in E. coli and formulated in Freund and Alum hydroxide adjuvants. Our results show that rPvRAP2 is immunogenic in both formulations, finding a trend of higher cytokine levels in immunized monkeys, specially in IL-4 levels (using Freund's adjuvant) and IL-5 (using Alum hydroxide). RAP2 is suggested as a P. vivax-vaccine candidate since immunized monkeys exhibited lower parasitemias than control groups after being experimentally challenged with the P. vivax VCG-I strain.

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Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys: A potential vaccine candidate

Abstract

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