IL-10, IFN-γ and IL-1β polymorphisms influence the development of OT in a Colombian population

C Naranjo-Galvis, Alejandra De-la-Torre, E Mantilla-Muriel, L Beltrán-Angarita, X Elcoroaristizabal-Martín, R McLeod, N Alliey-Rodriguez, I Begeman, C López de Mesa, JE Gómez-Marín

Research output: Contribution to journalResearch Articlepeer-review

Abstract

Background: Toxoplasmosis is caused by infection with the protozoan parasite Toxoplasma gondii which has the capacity to infect all warm-blooded animals worldwide. It is estimated that 30-70% of the human population is infected with this parasite, and essentially the entire human population is at risk of infection. A limited number of people develop symptoms, suggesting that host susceptibility and strain disparity can play a role in the variability of clinical symptoms. Toxoplasmosis is a major cause of visual defects in the Colombian population, however, the association between genetic polymorphisms in cytokine genes and susceptibility to ocular toxoplasmosis has not been studied in this population.

Methods & Materials: This work evaluates the associations between polymorphisms in genes coding for cytokines TNF-α (rs1799964, rs1800629, rs1799724, rs1800630, rs361525); IL-1β (rs16944, rs1143634, rs1143627), IL-1α (rs1800587); IFN-γ (rs2430561); IL-10 (rs1800896, rs1800871), and the presence of ocular toxoplasmosis (OT) in a sample of Colombian population (61 patients with OT and 116 healthy controls). Genotyping was performed with the “ddNTP primer extension” technique. Functional effect predictions of SNPs were done using FuncPred.

Results: Genotype distribution of all polymorphisms did not deviate significantly from the Hardy-Weinberg equilibrium. Significant higher frequencies in IL-1β, IFN-γ and IL-10 polymorphisms were observed in the OT group. A polymorphism in the IL-10 gene-promoter (-1082G/A) was significantly more prevalent in OT patients than in controls (P = 1.93e-08; OR = 5.27e + 03; 95%CI = 3.18-8.739; pBONF = 3.48e-07). In contrast, the haplotype “AG” of the IL-10 gene promoter polymorphism (rs1800896, rs1800871) was present with lower frequency in OT patients [P = 7e-04, OR (95%CI) 0.10 (0.03-0.35)]. The polymorphism (+ 874 A/T) of IFN-γ was associated with OT (P = 3.37e-05; OR = 4.2; 95%CI = 2.478-7.12; pBONF = 6.07e-04). The haplotype “GAG” of the IL-1β gene promoter polymorphisms (rs1143634, rs1143627, rs16944), appeared to be significantly associated with OT (P = 0.0494).

Conclusion: The IL-10, IFN-γ and IL-1β polymorphisms influence development of OT in the Colombian population. Our study provides evidence that common genetic variants in Th1 (IL-1, IFN-γ, TNF-α) and Th2 (IL10) genes are associated with risk to develop OT in patients from Colombia.
Original languageEnglish (US)
Pages (from-to)16-17
Number of pages2
JournalInternational Journal of Infectious Diseases
Volume73
DOIs
StatePublished - 2018

All Science Journal Classification (ASJC) codes

  • General Neuroscience

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