Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion

Diana P. Díaz, Marisol Ocampo, Yahson Varela, Hernando Curtidor, Manuel A. Patarroyo, Manuel E. Patarroyo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

© 2017 Springer Science+Business Media New YorkThis study was aimed at characterising the PPE7 protein from the PE/PPE protein family. The presence and transcription of the rv0354c gene in the Mycobacterium tuberculosis complex was determined and the subcellular localisation of the PPE7 protein on mycobacterial membrane was confirmed by immunoelectron microscope. Two peptides were identified as having high binding activity (HABPs) and were tested in vitro regarding the invasion of Mycobacterium tuberculosis H37Rv. HABP 39224 inhibited invasion in A549 epithelial cells and U937 macrophages by more than 50%, whilst HABP 39225 inhibited invasion by 40% in U937 cells. HABP 39224, located in the protein’s C-terminal region, has a completely conserved amino acid sequence in M. tuberculosis complex species and could be selected as a base peptide when designing a subunit-based, anti-tuberculosis vaccine.
Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalMolecular and Cellular Biochemistry
DOIs
StatePublished - Feb 15 2017

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Mycobacterium tuberculosis
Peptides
Tuberculosis Vaccines
U937 Cells
Proteins
Protein C
Macrophages
Amino Acid Sequence
Transcription
Epithelial Cells
Microscopes
Membranes
Genes
Amino Acids
Industry

Cite this

Díaz, Diana P. ; Ocampo, Marisol ; Varela, Yahson ; Curtidor, Hernando ; Patarroyo, Manuel A. ; Patarroyo, Manuel E. / Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion. In: Molecular and Cellular Biochemistry. 2017 ; pp. 1-12.
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abstract = "{\circledC} 2017 Springer Science+Business Media New YorkThis study was aimed at characterising the PPE7 protein from the PE/PPE protein family. The presence and transcription of the rv0354c gene in the Mycobacterium tuberculosis complex was determined and the subcellular localisation of the PPE7 protein on mycobacterial membrane was confirmed by immunoelectron microscope. Two peptides were identified as having high binding activity (HABPs) and were tested in vitro regarding the invasion of Mycobacterium tuberculosis H37Rv. HABP 39224 inhibited invasion in A549 epithelial cells and U937 macrophages by more than 50{\%}, whilst HABP 39225 inhibited invasion by 40{\%} in U937 cells. HABP 39224, located in the protein’s C-terminal region, has a completely conserved amino acid sequence in M. tuberculosis complex species and could be selected as a base peptide when designing a subunit-based, anti-tuberculosis vaccine.",
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Identifying and characterising PPE7 (Rv0354c) high activity binding peptides and their role in inhibiting cell invasion. / Díaz, Diana P.; Ocampo, Marisol; Varela, Yahson; Curtidor, Hernando; Patarroyo, Manuel A.; Patarroyo, Manuel E.

In: Molecular and Cellular Biochemistry, 15.02.2017, p. 1-12.

Research output: Contribution to journalArticle

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AB - © 2017 Springer Science+Business Media New YorkThis study was aimed at characterising the PPE7 protein from the PE/PPE protein family. The presence and transcription of the rv0354c gene in the Mycobacterium tuberculosis complex was determined and the subcellular localisation of the PPE7 protein on mycobacterial membrane was confirmed by immunoelectron microscope. Two peptides were identified as having high binding activity (HABPs) and were tested in vitro regarding the invasion of Mycobacterium tuberculosis H37Rv. HABP 39224 inhibited invasion in A549 epithelial cells and U937 macrophages by more than 50%, whilst HABP 39225 inhibited invasion by 40% in U937 cells. HABP 39224, located in the protein’s C-terminal region, has a completely conserved amino acid sequence in M. tuberculosis complex species and could be selected as a base peptide when designing a subunit-based, anti-tuberculosis vaccine.

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