Identification of bat trypanosomes from Minas Gerais state, Brazil, based on 18S rDNA and Cathepsin-L-like targets

Elaine Cristina Bento, César Gómez-Hernández, Lara Rocha Batista, Laís Anversa, André Luiz Pedrosa, Eliane Lages-Silva, Juan David Ramírez, Luis Eduardo Ramirez

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Several bat species can be infected by trypanosomes, but there is not much information about which of these parasites infect bats from Triângulo Mineiro and Alto Paranaíba, Minas Gerais state, Brazil, a formerly endemic region for Trypanosoma cruzi, the causative agent of Chagas disease. The aim of this study was to describe, characterize, and identify the presence of trypanosomes in bats. The captured bats (448) belong to four families and to 19 different species. Of those, 37 bats were found to be positive for trypanosomes by microhematocrit, (infection rate 8.3%) and 27 were positive after hemoculture analysis. Initially, the isolates were identified by PCR (18S rDNA, 24Sα rDNA, spliced leader, COII RFLP-PCR) using primers originally designed for T. cruzi. PCRs (18S rDNA, 24Sα rDNA) showed compatible bands for TcI, whereas COII RFLP-PCR showed a similar pattern associated to TcII. However, there was no DNA amplification using spliced leader as a target, revealing a discrepancy between the results. Phylogenetic analysis of Cathepsin L-like and 18S rDNA sequences proved that 15 of the isolates corresponded to Trypanosoma cruzi marinkellei and one to Trypanosoma dionisii. These results revealed that the diversity of trypanosome species in a region considered endemic for Chagas disease is greater than previous descriptions. All this can confirm the necessity of using DNA sequencing approaches in order to determinate trypanosomes species isolated from bats.

Original languageEnglish (US)
Pages (from-to)737-746
Number of pages10
JournalParasitology Research
Volume117
Issue number3
DOIs
StatePublished - Mar 1 2018

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Bento, E. C., Gómez-Hernández, C., Batista, L. R., Anversa, L., Pedrosa, A. L., Lages-Silva, E., ... Ramirez, L. E. (2018). Identification of bat trypanosomes from Minas Gerais state, Brazil, based on 18S rDNA and Cathepsin-L-like targets. Parasitology Research, 117(3), 737-746. https://doi.org/10.1007/s00436-017-5744-z
Bento, Elaine Cristina ; Gómez-Hernández, César ; Batista, Lara Rocha ; Anversa, Laís ; Pedrosa, André Luiz ; Lages-Silva, Eliane ; Ramírez, Juan David ; Ramirez, Luis Eduardo. / Identification of bat trypanosomes from Minas Gerais state, Brazil, based on 18S rDNA and Cathepsin-L-like targets. In: Parasitology Research. 2018 ; Vol. 117, No. 3. pp. 737-746.
@article{6741cea192d44376aeedefb10f27db9b,
title = "Identification of bat trypanosomes from Minas Gerais state, Brazil, based on 18S rDNA and Cathepsin-L-like targets",
abstract = "Several bat species can be infected by trypanosomes, but there is not much information about which of these parasites infect bats from Tri{\^a}ngulo Mineiro and Alto Parana{\'i}ba, Minas Gerais state, Brazil, a formerly endemic region for Trypanosoma cruzi, the causative agent of Chagas disease. The aim of this study was to describe, characterize, and identify the presence of trypanosomes in bats. The captured bats (448) belong to four families and to 19 different species. Of those, 37 bats were found to be positive for trypanosomes by microhematocrit, (infection rate 8.3{\%}) and 27 were positive after hemoculture analysis. Initially, the isolates were identified by PCR (18S rDNA, 24Sα rDNA, spliced leader, COII RFLP-PCR) using primers originally designed for T. cruzi. PCRs (18S rDNA, 24Sα rDNA) showed compatible bands for TcI, whereas COII RFLP-PCR showed a similar pattern associated to TcII. However, there was no DNA amplification using spliced leader as a target, revealing a discrepancy between the results. Phylogenetic analysis of Cathepsin L-like and 18S rDNA sequences proved that 15 of the isolates corresponded to Trypanosoma cruzi marinkellei and one to Trypanosoma dionisii. These results revealed that the diversity of trypanosome species in a region considered endemic for Chagas disease is greater than previous descriptions. All this can confirm the necessity of using DNA sequencing approaches in order to determinate trypanosomes species isolated from bats.",
author = "Bento, {Elaine Cristina} and C{\'e}sar G{\'o}mez-Hern{\'a}ndez and Batista, {Lara Rocha} and La{\'i}s Anversa and Pedrosa, {Andr{\'e} Luiz} and Eliane Lages-Silva and Ram{\'i}rez, {Juan David} and Ramirez, {Luis Eduardo}",
year = "2018",
month = "3",
day = "1",
doi = "10.1007/s00436-017-5744-z",
language = "English (US)",
volume = "117",
pages = "737--746",
journal = "Parasitology Research",
issn = "0932-0113",
publisher = "Springer",
number = "3",

}

Bento, EC, Gómez-Hernández, C, Batista, LR, Anversa, L, Pedrosa, AL, Lages-Silva, E, Ramírez, JD & Ramirez, LE 2018, 'Identification of bat trypanosomes from Minas Gerais state, Brazil, based on 18S rDNA and Cathepsin-L-like targets', Parasitology Research, vol. 117, no. 3, pp. 737-746. https://doi.org/10.1007/s00436-017-5744-z

Identification of bat trypanosomes from Minas Gerais state, Brazil, based on 18S rDNA and Cathepsin-L-like targets. / Bento, Elaine Cristina; Gómez-Hernández, César; Batista, Lara Rocha; Anversa, Laís; Pedrosa, André Luiz; Lages-Silva, Eliane; Ramírez, Juan David; Ramirez, Luis Eduardo.

In: Parasitology Research, Vol. 117, No. 3, 01.03.2018, p. 737-746.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of bat trypanosomes from Minas Gerais state, Brazil, based on 18S rDNA and Cathepsin-L-like targets

AU - Bento, Elaine Cristina

AU - Gómez-Hernández, César

AU - Batista, Lara Rocha

AU - Anversa, Laís

AU - Pedrosa, André Luiz

AU - Lages-Silva, Eliane

AU - Ramírez, Juan David

AU - Ramirez, Luis Eduardo

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Several bat species can be infected by trypanosomes, but there is not much information about which of these parasites infect bats from Triângulo Mineiro and Alto Paranaíba, Minas Gerais state, Brazil, a formerly endemic region for Trypanosoma cruzi, the causative agent of Chagas disease. The aim of this study was to describe, characterize, and identify the presence of trypanosomes in bats. The captured bats (448) belong to four families and to 19 different species. Of those, 37 bats were found to be positive for trypanosomes by microhematocrit, (infection rate 8.3%) and 27 were positive after hemoculture analysis. Initially, the isolates were identified by PCR (18S rDNA, 24Sα rDNA, spliced leader, COII RFLP-PCR) using primers originally designed for T. cruzi. PCRs (18S rDNA, 24Sα rDNA) showed compatible bands for TcI, whereas COII RFLP-PCR showed a similar pattern associated to TcII. However, there was no DNA amplification using spliced leader as a target, revealing a discrepancy between the results. Phylogenetic analysis of Cathepsin L-like and 18S rDNA sequences proved that 15 of the isolates corresponded to Trypanosoma cruzi marinkellei and one to Trypanosoma dionisii. These results revealed that the diversity of trypanosome species in a region considered endemic for Chagas disease is greater than previous descriptions. All this can confirm the necessity of using DNA sequencing approaches in order to determinate trypanosomes species isolated from bats.

AB - Several bat species can be infected by trypanosomes, but there is not much information about which of these parasites infect bats from Triângulo Mineiro and Alto Paranaíba, Minas Gerais state, Brazil, a formerly endemic region for Trypanosoma cruzi, the causative agent of Chagas disease. The aim of this study was to describe, characterize, and identify the presence of trypanosomes in bats. The captured bats (448) belong to four families and to 19 different species. Of those, 37 bats were found to be positive for trypanosomes by microhematocrit, (infection rate 8.3%) and 27 were positive after hemoculture analysis. Initially, the isolates were identified by PCR (18S rDNA, 24Sα rDNA, spliced leader, COII RFLP-PCR) using primers originally designed for T. cruzi. PCRs (18S rDNA, 24Sα rDNA) showed compatible bands for TcI, whereas COII RFLP-PCR showed a similar pattern associated to TcII. However, there was no DNA amplification using spliced leader as a target, revealing a discrepancy between the results. Phylogenetic analysis of Cathepsin L-like and 18S rDNA sequences proved that 15 of the isolates corresponded to Trypanosoma cruzi marinkellei and one to Trypanosoma dionisii. These results revealed that the diversity of trypanosome species in a region considered endemic for Chagas disease is greater than previous descriptions. All this can confirm the necessity of using DNA sequencing approaches in order to determinate trypanosomes species isolated from bats.

UR - http://www.scopus.com/inward/record.url?scp=85040567122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85040567122&partnerID=8YFLogxK

U2 - 10.1007/s00436-017-5744-z

DO - 10.1007/s00436-017-5744-z

M3 - Article

C2 - 29340783

AN - SCOPUS:85040567122

VL - 117

SP - 737

EP - 746

JO - Parasitology Research

JF - Parasitology Research

SN - 0932-0113

IS - 3

ER -

Bento EC, Gómez-Hernández C, Batista LR, Anversa L, Pedrosa AL, Lages-Silva E et al. Identification of bat trypanosomes from Minas Gerais state, Brazil, based on 18S rDNA and Cathepsin-L-like targets. Parasitology Research. 2018 Mar 1;117(3):737-746. https://doi.org/10.1007/s00436-017-5744-z