Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein

Carolina Saravia; Paola Martinez; Diana S. Granados; Carolina Lopez; Claudia Reyes; Manuel A. Patarroyo

doi:10.1016/j.bbrc.2008.10.153

Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein

Carolina Saravia
, Paola Martinez
, Diana S. Granados
, Carolina Lopez
, Claudia Reyes
, Manuel A. Patarroyo

urosario

Research output: Contribution to Journal › Research Article › peer-review

8 Scopus citations

Abstract

Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA_306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria.

Original language	English (US)
Pages (from-to)	1279-1283
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	377
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2008.10.153
State	Published - Dec 26 2008

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.10.153

Cite this

@article{9d340e96cae64b9da30a7d6cd44c35e0,

title = "Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein",

abstract = "Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50\%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria.",

author = "Carolina Saravia and Paola Martinez and Granados, \{Diana S.\} and Carolina Lopez and Claudia Reyes and Patarroyo, \{Manuel A.\}",

note = "Funding Information: Special gratitude goes to Professor Manuel Elkin Patarroyo for all his valuable comments. This research was supported by the {\textquoteleft}{\textquoteleft}Instituto Colombiano para el Desarrollo de la Ciencia {\textquoteleft}Francisco Jose de Caldas{\textquoteright} (COLCIENCIAS)” contract RC-2008. Nora Martinez patiently helped translating the manuscript and Alvaro Mongui helped with the artwork. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2008",

month = dec,

day = "26",

doi = "10.1016/j.bbrc.2008.10.153",

language = "English (US)",

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AU - Saravia, Carolina

AU - Martinez, Paola

AU - Granados, Diana S.

AU - Lopez, Carolina

AU - Reyes, Claudia

AU - Patarroyo, Manuel A.

N1 - Funding Information: Special gratitude goes to Professor Manuel Elkin Patarroyo for all his valuable comments. This research was supported by the ‘‘Instituto Colombiano para el Desarrollo de la Ciencia ‘Francisco Jose de Caldas’ (COLCIENCIAS)” contract RC-2008. Nora Martinez patiently helped translating the manuscript and Alvaro Mongui helped with the artwork. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2008/12/26

Y1 - 2008/12/26

N2 - Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria.

AB - Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria.

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SN - 0006-291X

VL - 377

SP - 1279

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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ER -

Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein

Abstract

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